Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1449 and Dsui_1450 are separated by 16 nucleotides Dsui_1450 and Dsui_1451 are separated by 22 nucleotides Dsui_1451 and Dsui_1452 are separated by 43 nucleotides
Dsui_1449: Dsui_1449 - protein kinase family protein, at 1,504,482 to 1,506,998
_1449
Dsui_1450: Dsui_1450 - serine/threonine protein phosphatase, at 1,507,015 to 1,507,842
_1450
Dsui_1451: Dsui_1451 - FHA domain-containing protein, at 1,507,865 to 1,508,593
_1451
Dsui_1452: Dsui_1452 - putative transmembrane sensor domain protein, at 1,508,637 to 1,510,850
_1452
Position (kb)
1507
1508
1509 Strain fitness (log2 ratio)
-2
-1
0
1 at 1506.904 kb on - strand at 1506.904 kb on - strand at 1507.025 kb on + strand at 1507.547 kb on + strand, within Dsui_1450 at 1507.631 kb on + strand, within Dsui_1450 at 1507.855 kb on + strand at 1507.863 kb on - strand at 1507.969 kb on - strand, within Dsui_1451 at 1508.005 kb on + strand, within Dsui_1451 at 1508.036 kb on + strand, within Dsui_1451 at 1508.036 kb on + strand, within Dsui_1451 at 1508.041 kb on + strand, within Dsui_1451 at 1508.058 kb on - strand at 1508.410 kb on - strand, within Dsui_1451 at 1508.587 kb on + strand at 1508.682 kb on - strand at 1508.689 kb on + strand at 1508.697 kb on - strand at 1509.164 kb on - strand, within Dsui_1452 at 1509.493 kb on + strand, within Dsui_1452
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 1,506,904 - -1.0 1,506,904 - +0.6 1,507,025 + +0.3 1,507,547 + Dsui_1450 0.64 +1.6 1,507,631 + Dsui_1450 0.74 -0.4 1,507,855 + -0.1 1,507,863 - -2.2 1,507,969 - Dsui_1451 0.14 -1.0 1,508,005 + Dsui_1451 0.19 -0.2 1,508,036 + Dsui_1451 0.23 -0.3 1,508,036 + Dsui_1451 0.23 -0.3 1,508,041 + Dsui_1451 0.24 -1.3 1,508,058 - +0.4 1,508,410 - Dsui_1451 0.75 -0.4 1,508,587 + -0.4 1,508,682 - -0.1 1,508,689 + -0.0 1,508,697 - -0.7 1,509,164 - Dsui_1452 0.24 -0.4 1,509,493 + Dsui_1452 0.39 -0.2
Or see this region's nucleotide sequence