Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1092 and Dsui_1093 are separated by 115 nucleotides Dsui_1093 and Dsui_1094 overlap by 4 nucleotides Dsui_1094 and Dsui_1095 overlap by 4 nucleotides
Dsui_1092: Dsui_1092 - Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family, at 1,131,720 to 1,132,877
_1092
Dsui_1093: Dsui_1093 - hypothetical protein, at 1,132,993 to 1,133,337
_1093
Dsui_1094: Dsui_1094 - shikimate kinase, at 1,133,334 to 1,133,906
_1094
Dsui_1095: Dsui_1095 - 3-dehydroquinate synthase, at 1,133,903 to 1,134,997
_1095
Position (kb)
1133
1134 Strain fitness (log2 ratio)
-1
0
1
2 at 1132.909 kb on - strand at 1132.915 kb on + strand at 1132.978 kb on + strand at 1133.033 kb on - strand, within Dsui_1093 at 1133.066 kb on + strand, within Dsui_1093 at 1133.066 kb on + strand, within Dsui_1093 at 1133.110 kb on + strand, within Dsui_1093 at 1133.134 kb on + strand, within Dsui_1093 at 1133.134 kb on + strand, within Dsui_1093 at 1133.134 kb on + strand, within Dsui_1093 at 1133.134 kb on + strand, within Dsui_1093 at 1133.144 kb on + strand at 1133.190 kb on + strand, within Dsui_1093 at 1133.190 kb on + strand, within Dsui_1093 at 1133.223 kb on + strand, within Dsui_1093 at 1133.784 kb on + strand, within Dsui_1094 at 1133.858 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 1,132,909 - -0.2 1,132,915 + -0.9 1,132,978 + +0.4 1,133,033 - Dsui_1093 0.12 -0.5 1,133,066 + Dsui_1093 0.21 -0.4 1,133,066 + Dsui_1093 0.21 +0.4 1,133,110 + Dsui_1093 0.34 +0.4 1,133,134 + Dsui_1093 0.41 +1.8 1,133,134 + Dsui_1093 0.41 -1.1 1,133,134 + Dsui_1093 0.41 -0.2 1,133,134 + Dsui_1093 0.41 +0.2 1,133,144 + -1.4 1,133,190 + Dsui_1093 0.57 -0.1 1,133,190 + Dsui_1093 0.57 +0.2 1,133,223 + Dsui_1093 0.67 -0.2 1,133,784 + Dsui_1094 0.79 -0.8 1,133,858 + -0.0
Or see this region's nucleotide sequence