Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0940 and Dsui_0941 are separated by 152 nucleotides Dsui_0941 and Dsui_0942 are separated by 153 nucleotides Dsui_0942 and Dsui_0943 are separated by 333 nucleotides
Dsui_0940: Dsui_0940 - Nif-specific regulatory protein, at 968,329 to 970,062
_0940
Dsui_0941: Dsui_0941 - nitrogenase-associated protein, at 970,215 to 970,643
_0941
Dsui_0942: Dsui_0942 - hypothetical protein, at 970,797 to 971,366
_0942
Dsui_0943: Dsui_0943 - nitrogenase cofactor biosynthesis protein NifB, at 971,700 to 973,223
_0943
Position (kb)
970
971
972 Strain fitness (log2 ratio)
-2
-1
0
1 at 969.978 kb on - strand at 969.983 kb on - strand at 969.998 kb on + strand at 970.121 kb on - strand at 970.337 kb on + strand at 970.414 kb on + strand, within Dsui_0941 at 970.434 kb on - strand at 970.469 kb on + strand, within Dsui_0941 at 970.581 kb on - strand, within Dsui_0941 at 970.853 kb on - strand at 970.954 kb on + strand, within Dsui_0942 at 970.962 kb on - strand, within Dsui_0942 at 970.977 kb on + strand, within Dsui_0942 at 971.009 kb on + strand, within Dsui_0942 at 971.038 kb on + strand at 971.104 kb on - strand, within Dsui_0942 at 971.214 kb on - strand, within Dsui_0942 at 971.284 kb on + strand, within Dsui_0942 at 971.635 kb on - strand at 972.132 kb on + strand, within Dsui_0943 at 972.137 kb on + strand, within Dsui_0943 at 972.235 kb on + strand, within Dsui_0943 at 972.239 kb on + strand, within Dsui_0943 at 972.317 kb on - strand, within Dsui_0943
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 969,978 - -0.2 969,983 - -0.3 969,998 + -0.2 970,121 - -0.8 970,337 + +0.4 970,414 + Dsui_0941 0.46 -0.4 970,434 - +0.4 970,469 + Dsui_0941 0.59 -0.7 970,581 - Dsui_0941 0.85 +0.8 970,853 - -0.5 970,954 + Dsui_0942 0.28 +1.1 970,962 - Dsui_0942 0.29 -0.8 970,977 + Dsui_0942 0.32 -0.2 971,009 + Dsui_0942 0.37 -0.2 971,038 + -0.3 971,104 - Dsui_0942 0.54 -1.0 971,214 - Dsui_0942 0.73 -1.9 971,284 + Dsui_0942 0.85 -1.9 971,635 - -0.9 972,132 + Dsui_0943 0.28 +0.2 972,137 + Dsui_0943 0.29 +0.0 972,235 + Dsui_0943 0.35 +0.1 972,239 + Dsui_0943 0.35 -2.6 972,317 - Dsui_0943 0.40 -0.3
Or see this region's nucleotide sequence