Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0459 and Dsui_0460 are separated by 25 nucleotides Dsui_0460 and Dsui_0461 overlap by 4 nucleotides
Dsui_0459: Dsui_0459 - DNA polymerase I, at 462,976 to 465,708
_0459
Dsui_0460: Dsui_0460 - ADP-heptose:LPS heptosyltransferase, at 465,734 to 466,699
_0460
Dsui_0461: Dsui_0461 - glycosyltransferase, at 466,696 to 467,832
_0461
Position (kb) 465
466
467 Strain fitness (log2 ratio) -1
0
1
2 at 465.115 kb on + strand, within Dsui_0459 at 465.178 kb on - strand at 465.213 kb on + strand, within Dsui_0459 at 465.545 kb on + strand at 465.812 kb on + strand at 465.820 kb on - strand at 465.888 kb on - strand, within Dsui_0460 at 465.940 kb on - strand, within Dsui_0460 at 466.153 kb on + strand at 466.448 kb on + strand, within Dsui_0460 at 466.642 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.652 kb on + strand at 466.658 kb on - strand at 466.658 kb on - strand at 466.758 kb on - strand at 466.795 kb on + strand at 466.810 kb on + strand, within Dsui_0461 at 466.880 kb on + strand, within Dsui_0461 at 466.880 kb on + strand, within Dsui_0461 at 466.880 kb on + strand, within Dsui_0461 at 466.903 kb on + strand, within Dsui_0461 at 466.947 kb on + strand, within Dsui_0461 at 467.042 kb on - strand, within Dsui_0461 at 467.107 kb on - strand, within Dsui_0461 at 467.130 kb on - strand, within Dsui_0461 at 467.258 kb on - strand, within Dsui_0461 at 467.447 kb on + strand, within Dsui_0461 at 467.449 kb on + strand, within Dsui_0461 at 467.508 kb on + strand, within Dsui_0461 at 467.532 kb on - strand, within Dsui_0461 at 467.605 kb on + strand, within Dsui_0461 at 467.699 kb on - strand, within Dsui_0461
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 465,115 + Dsui_0459 0.78 +1.0 465,178 - +2.0 465,213 + Dsui_0459 0.82 +2.0 465,545 + +1.0 465,812 + -0.0 465,820 - -0.1 465,888 - Dsui_0460 0.16 -1.1 465,940 - Dsui_0460 0.21 -0.7 466,153 + +0.7 466,448 + Dsui_0460 0.74 +0.2 466,642 + -1.1 466,650 + -0.5 466,650 + +0.8 466,650 + -1.4 466,650 + -0.3 466,652 + +0.4 466,658 - -0.2 466,658 - -0.7 466,758 - -0.8 466,795 + -0.4 466,810 + Dsui_0461 0.10 -0.2 466,880 + Dsui_0461 0.16 -1.2 466,880 + Dsui_0461 0.16 -0.7 466,880 + Dsui_0461 0.16 -0.3 466,903 + Dsui_0461 0.18 -0.3 466,947 + Dsui_0461 0.22 -0.7 467,042 - Dsui_0461 0.30 +0.1 467,107 - Dsui_0461 0.36 -0.5 467,130 - Dsui_0461 0.38 -0.5 467,258 - Dsui_0461 0.49 +0.4 467,447 + Dsui_0461 0.66 -0.6 467,449 + Dsui_0461 0.66 +0.1 467,508 + Dsui_0461 0.71 +0.0 467,532 - Dsui_0461 0.74 +0.2 467,605 + Dsui_0461 0.80 -0.7 467,699 - Dsui_0461 0.88 +0.1
Or see this region's nucleotide sequence