Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0441 and Dsui_0442 are separated by 159 nucleotides Dsui_0442 and Dsui_0443 are separated by 6 nucleotides Dsui_0443 and Dsui_0444 are separated by 176 nucleotides
Dsui_0441: Dsui_0441 - Mg2+ transporter MgtE, at 448,010 to 449,452
_0441
Dsui_0442: Dsui_0442 - monofunctional biosynthetic peptidoglycan transglycosylase, at 449,612 to 450,304
_0442
Dsui_0443: Dsui_0443 - shikimate 5-dehydrogenase, at 450,311 to 451,126
_0443
Dsui_0444: Dsui_0444 - hemerythrin-like metal-binding domain-containing protein, at 451,303 to 451,761
_0444
Position (kb) 449
450
451 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 448.973 kb on - strand, within Dsui_0441 at 449.514 kb on + strand at 449.522 kb on - strand at 449.565 kb on - strand at 449.565 kb on - strand at 449.737 kb on + strand, within Dsui_0442 at 450.036 kb on + strand, within Dsui_0442 at 450.037 kb on - strand, within Dsui_0442 at 450.037 kb on - strand, within Dsui_0442 at 450.101 kb on - strand, within Dsui_0442 at 450.217 kb on + strand, within Dsui_0442 at 450.220 kb on - strand, within Dsui_0442 at 450.220 kb on - strand, within Dsui_0442 at 450.288 kb on - strand at 451.165 kb on + strand at 451.174 kb on - strand at 451.199 kb on - strand at 451.209 kb on - strand at 451.266 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 448,973 - Dsui_0441 0.67 +2.0 449,514 + +0.9 449,522 - +0.2 449,565 - -0.1 449,565 - -0.1 449,737 + Dsui_0442 0.18 -0.2 450,036 + Dsui_0442 0.61 -1.2 450,037 - Dsui_0442 0.61 -1.0 450,037 - Dsui_0442 0.61 -2.9 450,101 - Dsui_0442 0.71 -0.1 450,217 + Dsui_0442 0.87 -0.6 450,220 - Dsui_0442 0.88 +0.2 450,220 - Dsui_0442 0.88 -0.6 450,288 - -1.2 451,165 + +0.2 451,174 - -1.2 451,199 - -0.1 451,209 - -0.0 451,266 + -0.6
Or see this region's nucleotide sequence