Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0195 and Dsui_0196 are separated by 73 nucleotides Dsui_0196 and Dsui_0197 are separated by 69 nucleotides Dsui_0197 and Dsui_0198 are separated by 6 nucleotides Dsui_0198 and Dsui_0199 overlap by 4 nucleotides
Dsui_0195: Dsui_0195 - Caudovirus prohead protease, at 196,123 to 197,463
_0195
Dsui_0196: Dsui_0196 - hypothetical protein, at 197,537 to 199,060
_0196
Dsui_0197: Dsui_0197 - hypothetical protein, at 199,130 to 199,480
_0197
Dsui_0198: Dsui_0198 - phage conserved hypothetical protein, phiE125 gp8 family, at 199,487 to 200,035
_0198
Dsui_0199: Dsui_0199 - Bacteriophage protein of unknown function (DUF646), at 200,032 to 200,517
_0199
Position (kb) 197
198
199
200 Strain fitness (log2 ratio) -2
-1
0
1
2 at 196.698 kb on - strand, within Dsui_0195 at 196.712 kb on - strand, within Dsui_0195 at 196.757 kb on + strand, within Dsui_0195 at 196.775 kb on - strand, within Dsui_0195 at 196.933 kb on - strand, within Dsui_0195 at 197.133 kb on + strand, within Dsui_0195 at 197.178 kb on - strand, within Dsui_0195 at 197.189 kb on - strand, within Dsui_0195 at 197.276 kb on - strand, within Dsui_0195 at 197.302 kb on + strand, within Dsui_0195 at 197.302 kb on + strand, within Dsui_0195 at 197.315 kb on - strand, within Dsui_0195 at 197.327 kb on - strand, within Dsui_0195 at 197.380 kb on + strand at 197.405 kb on - strand at 197.541 kb on - strand at 197.590 kb on - strand at 197.660 kb on + strand at 197.685 kb on + strand at 197.688 kb on - strand at 197.759 kb on + strand, within Dsui_0196 at 197.881 kb on - strand, within Dsui_0196 at 197.889 kb on + strand, within Dsui_0196 at 197.889 kb on + strand, within Dsui_0196 at 197.926 kb on + strand, within Dsui_0196 at 197.932 kb on + strand, within Dsui_0196 at 197.935 kb on - strand, within Dsui_0196 at 197.990 kb on - strand, within Dsui_0196 at 198.032 kb on - strand, within Dsui_0196 at 198.032 kb on - strand, within Dsui_0196 at 198.071 kb on + strand, within Dsui_0196 at 198.126 kb on + strand, within Dsui_0196 at 198.160 kb on - strand, within Dsui_0196 at 198.245 kb on + strand, within Dsui_0196 at 198.273 kb on - strand, within Dsui_0196 at 198.293 kb on - strand, within Dsui_0196 at 198.333 kb on + strand, within Dsui_0196 at 198.423 kb on - strand, within Dsui_0196 at 198.680 kb on - strand, within Dsui_0196 at 198.891 kb on - strand, within Dsui_0196 at 198.891 kb on - strand, within Dsui_0196 at 199.089 kb on + strand at 199.089 kb on + strand at 199.153 kb on - strand at 199.265 kb on - strand, within Dsui_0197 at 199.274 kb on - strand, within Dsui_0197 at 199.537 kb on - strand at 199.556 kb on + strand, within Dsui_0198 at 199.645 kb on + strand, within Dsui_0198 at 199.691 kb on - strand, within Dsui_0198 at 199.884 kb on - strand, within Dsui_0198 at 199.884 kb on - strand, within Dsui_0198 at 199.933 kb on - strand, within Dsui_0198
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 196,698 - Dsui_0195 0.43 +0.2 196,712 - Dsui_0195 0.44 -0.3 196,757 + Dsui_0195 0.47 -0.4 196,775 - Dsui_0195 0.49 +1.3 196,933 - Dsui_0195 0.60 +0.4 197,133 + Dsui_0195 0.75 +2.7 197,178 - Dsui_0195 0.79 +0.0 197,189 - Dsui_0195 0.79 -0.6 197,276 - Dsui_0195 0.86 -0.3 197,302 + Dsui_0195 0.88 -0.5 197,302 + Dsui_0195 0.88 -1.8 197,315 - Dsui_0195 0.89 -0.4 197,327 - Dsui_0195 0.90 +0.6 197,380 + +0.1 197,405 - -0.3 197,541 - -0.0 197,590 - -0.4 197,660 + +0.6 197,685 + +0.7 197,688 - +0.1 197,759 + Dsui_0196 0.15 -0.7 197,881 - Dsui_0196 0.23 -0.3 197,889 + Dsui_0196 0.23 +0.1 197,889 + Dsui_0196 0.23 -1.3 197,926 + Dsui_0196 0.26 -0.7 197,932 + Dsui_0196 0.26 -0.0 197,935 - Dsui_0196 0.26 -0.7 197,990 - Dsui_0196 0.30 -1.4 198,032 - Dsui_0196 0.32 -0.4 198,032 - Dsui_0196 0.32 -0.4 198,071 + Dsui_0196 0.35 -1.3 198,126 + Dsui_0196 0.39 -0.3 198,160 - Dsui_0196 0.41 -0.6 198,245 + Dsui_0196 0.46 -0.8 198,273 - Dsui_0196 0.48 +0.8 198,293 - Dsui_0196 0.50 -0.2 198,333 + Dsui_0196 0.52 +0.3 198,423 - Dsui_0196 0.58 -0.1 198,680 - Dsui_0196 0.75 -0.7 198,891 - Dsui_0196 0.89 -0.0 198,891 - Dsui_0196 0.89 -1.2 199,089 + -1.5 199,089 + -0.9 199,153 - -0.3 199,265 - Dsui_0197 0.38 +0.2 199,274 - Dsui_0197 0.41 -0.1 199,537 - +0.3 199,556 + Dsui_0198 0.13 +1.1 199,645 + Dsui_0198 0.29 +0.2 199,691 - Dsui_0198 0.37 +0.7 199,884 - Dsui_0198 0.72 +0.5 199,884 - Dsui_0198 0.72 -0.3 199,933 - Dsui_0198 0.81 +0.6
Or see this region's nucleotide sequence