Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0034 and Dsui_0035 are separated by 267 nucleotides Dsui_0035 and Dsui_0036 are separated by 115 nucleotides Dsui_0036 and Dsui_0037 are separated by 86 nucleotides
Dsui_0034: Dsui_0034 - flagellar capping protein, at 30,101 to 31,537
_0034
Dsui_0035: Dsui_0035 - ankyrin repeat-containing protein, at 31,805 to 32,854
_0035
Dsui_0036: Dsui_0036 - TonB family protein, at 32,970 to 33,542
_0036
Dsui_0037: Dsui_0037 - putative acyl-CoA transferase/carnitine dehydratase, at 33,629 to 34,840
_0037
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1 at 30.808 kb on - strand, within Dsui_0034 at 30.947 kb on + strand, within Dsui_0034 at 30.968 kb on + strand, within Dsui_0034 at 31.022 kb on - strand, within Dsui_0034 at 31.104 kb on - strand, within Dsui_0034 at 31.157 kb on + strand, within Dsui_0034 at 31.215 kb on - strand, within Dsui_0034 at 31.328 kb on + strand at 31.391 kb on + strand at 31.399 kb on - strand at 31.417 kb on + strand at 31.484 kb on - strand at 31.549 kb on + strand at 31.569 kb on + strand at 31.604 kb on - strand at 31.622 kb on + strand at 31.630 kb on - strand at 31.827 kb on + strand at 31.835 kb on - strand at 31.883 kb on - strand at 32.118 kb on - strand, within Dsui_0035 at 32.129 kb on - strand, within Dsui_0035 at 32.187 kb on - strand, within Dsui_0035 at 32.206 kb on + strand, within Dsui_0035 at 32.207 kb on + strand at 32.210 kb on - strand, within Dsui_0035 at 32.210 kb on - strand, within Dsui_0035 at 32.215 kb on - strand at 32.215 kb on - strand, within Dsui_0035 at 32.215 kb on - strand, within Dsui_0035 at 32.230 kb on - strand, within Dsui_0035 at 32.280 kb on - strand, within Dsui_0035 at 32.282 kb on + strand, within Dsui_0035 at 32.332 kb on - strand, within Dsui_0035 at 32.433 kb on + strand at 32.477 kb on + strand at 32.486 kb on + strand, within Dsui_0035 at 32.618 kb on - strand, within Dsui_0035 at 32.660 kb on + strand at 32.671 kb on + strand at 32.671 kb on + strand at 32.758 kb on - strand at 32.834 kb on - strand at 33.188 kb on + strand, within Dsui_0036 at 33.188 kb on + strand, within Dsui_0036 at 33.261 kb on - strand, within Dsui_0036 at 33.273 kb on - strand, within Dsui_0036 at 33.332 kb on + strand, within Dsui_0036 at 33.353 kb on - strand, within Dsui_0036 at 33.551 kb on + strand at 33.559 kb on - strand at 33.671 kb on + strand at 33.715 kb on + strand at 33.786 kb on - strand, within Dsui_0037 at 33.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 30,808 - Dsui_0034 0.49 +0.0 30,947 + Dsui_0034 0.59 -0.5 30,968 + Dsui_0034 0.60 -1.0 31,022 - Dsui_0034 0.64 -0.1 31,104 - Dsui_0034 0.70 -0.8 31,157 + Dsui_0034 0.73 -0.1 31,215 - Dsui_0034 0.78 -0.3 31,328 + -0.2 31,391 + -0.8 31,399 - -0.4 31,417 + -1.0 31,484 - -0.5 31,549 + -0.1 31,569 + -0.5 31,604 - +0.2 31,622 + -0.3 31,630 - +0.7 31,827 + -0.3 31,835 - +0.1 31,883 - +0.2 32,118 - Dsui_0035 0.30 -1.3 32,129 - Dsui_0035 0.31 -0.4 32,187 - Dsui_0035 0.36 -0.6 32,206 + Dsui_0035 0.38 +0.0 32,207 + -2.3 32,210 - Dsui_0035 0.39 -0.0 32,210 - Dsui_0035 0.39 -0.7 32,215 - -1.3 32,215 - Dsui_0035 0.39 -0.2 32,215 - Dsui_0035 0.39 -2.0 32,230 - Dsui_0035 0.40 -0.4 32,280 - Dsui_0035 0.45 +0.4 32,282 + Dsui_0035 0.45 -0.5 32,332 - Dsui_0035 0.50 -0.2 32,433 + -1.3 32,477 + -2.2 32,486 + Dsui_0035 0.65 -0.4 32,618 - Dsui_0035 0.77 -0.1 32,660 + -1.2 32,671 + -2.2 32,671 + -1.0 32,758 - -0.4 32,834 - -1.8 33,188 + Dsui_0036 0.38 -0.3 33,188 + Dsui_0036 0.38 -0.5 33,261 - Dsui_0036 0.51 -0.3 33,273 - Dsui_0036 0.53 -0.8 33,332 + Dsui_0036 0.63 +0.5 33,353 - Dsui_0036 0.67 -0.2 33,551 + -0.4 33,559 - -0.2 33,671 + -0.3 33,715 + -0.2 33,786 - Dsui_0037 0.13 -0.5 33,851 + +0.0
Or see this region's nucleotide sequence