Experiment: m.b. Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2838 and Dshi_2839 are separated by 89 nucleotides Dshi_2839 and Dshi_2840 are separated by 4 nucleotides Dshi_2840 and Dshi_2841 overlap by 4 nucleotides Dshi_2841 and Dshi_2842 are separated by 181 nucleotides
Dshi_2838: Dshi_2838 - UspA domain protein (RefSeq), at 2,996,802 to 2,997,257
_2838
Dshi_2839: Dshi_2839 - Scaffold protein Nfu/NifU (RefSeq), at 2,997,347 to 2,997,910
_2839
Dshi_2840: Dshi_2840 - peptidase M22 glycoprotease (RefSeq), at 2,997,915 to 2,998,571
_2840
Dshi_2841: Dshi_2841 - ribosomal-protein-alanine acetyltransferase (RefSeq), at 2,998,568 to 2,998,987
_2841
Dshi_2842: Dshi_2842 - basic membrane lipoprotein (RefSeq), at 2,999,169 to 3,000,158
_2842
Position (kb)
2997
2998
2999 Strain fitness (log2 ratio)
-2
-1
0
1 at 2996.920 kb on - strand, within Dshi_2838 at 2996.952 kb on - strand, within Dshi_2838 at 2997.275 kb on - strand at 2998.621 kb on + strand, within Dshi_2841 at 2998.792 kb on - strand, within Dshi_2841 at 2998.821 kb on - strand, within Dshi_2841 at 2998.874 kb on + strand, within Dshi_2841 at 2998.874 kb on + strand, within Dshi_2841 at 2998.898 kb on - strand, within Dshi_2841 at 2999.083 kb on - strand at 2999.125 kb on + strand at 2999.242 kb on - strand at 2999.320 kb on - strand, within Dshi_2842 at 2999.493 kb on + strand, within Dshi_2842
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1 mM remove 2,996,920 - Dshi_2838 0.26 -0.1 2,996,952 - Dshi_2838 0.33 +0.4 2,997,275 - -0.7 2,998,621 + Dshi_2841 0.13 -0.7 2,998,792 - Dshi_2841 0.53 +1.2 2,998,821 - Dshi_2841 0.60 -1.8 2,998,874 + Dshi_2841 0.73 -1.9 2,998,874 + Dshi_2841 0.73 -0.9 2,998,898 - Dshi_2841 0.79 -0.7 2,999,083 - +0.6 2,999,125 + +0.2 2,999,242 - +1.1 2,999,320 - Dshi_2842 0.15 +0.7 2,999,493 + Dshi_2842 0.33 +1.0
Or see this region's nucleotide sequence