Experiment: m.b. Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0331 and Dshi_0332 overlap by 4 nucleotides Dshi_0332 and Dshi_0333 are separated by 62 nucleotides Dshi_0333 and Dshi_R0004 are separated by 155 nucleotides Dshi_R0004 and Dshi_0334 are separated by 51 nucleotides Dshi_0334 and Dshi_0335 are separated by 36 nucleotides
Dshi_0331: Dshi_0331 - protein of unknown function DUF37 (RefSeq), at 319,810 to 320,031
_0331
Dshi_0332: Dshi_0332 - ribonuclease P protein component (RefSeq), at 320,028 to 320,417
_0332
Dshi_0333: Dshi_0333 - ribosomal protein L34 (RefSeq), at 320,480 to 320,614
_0333
Dshi_R0004: Dshi_R0004 - tRNA-Arg (RefSeq), at 320,770 to 320,846
_R0004
Dshi_0334: Dshi_0334 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 320,898 to 322,301
_0334
Dshi_0335: Dshi_0335 - pyridine nucleotide-disulphide oxidoreductase dimerisation region (RefSeq), at 322,338 to 323,750
_0335
Position (kb) 320
321
322
323 Strain fitness (log2 ratio) -2
-1
0
1 at 319.953 kb on + strand, within Dshi_0331 at 319.983 kb on + strand, within Dshi_0331 at 321.235 kb on + strand, within Dshi_0334 at 321.243 kb on - strand, within Dshi_0334 at 321.379 kb on - strand, within Dshi_0334 at 321.490 kb on - strand, within Dshi_0334 at 321.563 kb on + strand, within Dshi_0334 at 321.563 kb on + strand, within Dshi_0334 at 321.578 kb on + strand, within Dshi_0334 at 321.748 kb on + strand, within Dshi_0334 at 321.962 kb on - strand, within Dshi_0334 at 321.985 kb on - strand, within Dshi_0334 at 322.001 kb on + strand, within Dshi_0334 at 322.001 kb on + strand, within Dshi_0334 at 322.001 kb on + strand, within Dshi_0334 at 322.009 kb on - strand, within Dshi_0334 at 322.029 kb on + strand, within Dshi_0334 at 322.078 kb on + strand, within Dshi_0334 at 322.080 kb on + strand, within Dshi_0334 at 322.162 kb on - strand at 322.334 kb on - strand at 322.381 kb on + strand at 322.439 kb on + strand at 322.439 kb on + strand at 322.439 kb on + strand at 322.447 kb on - strand at 322.447 kb on - strand at 322.447 kb on - strand at 322.512 kb on - strand, within Dshi_0335 at 322.589 kb on + strand, within Dshi_0335 at 322.708 kb on + strand, within Dshi_0335 at 322.716 kb on - strand, within Dshi_0335 at 322.742 kb on - strand, within Dshi_0335 at 322.807 kb on + strand, within Dshi_0335 at 323.157 kb on - strand, within Dshi_0335 at 323.165 kb on - strand, within Dshi_0335 at 323.173 kb on - strand, within Dshi_0335 at 323.187 kb on + strand, within Dshi_0335 at 323.263 kb on - strand, within Dshi_0335 at 323.263 kb on - strand, within Dshi_0335 at 323.263 kb on - strand, within Dshi_0335 at 323.263 kb on - strand, within Dshi_0335
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1 mM remove 319,953 + Dshi_0331 0.64 -0.6 319,983 + Dshi_0331 0.78 -0.9 321,235 + Dshi_0334 0.24 +0.9 321,243 - Dshi_0334 0.25 -0.1 321,379 - Dshi_0334 0.34 +0.9 321,490 - Dshi_0334 0.42 +1.0 321,563 + Dshi_0334 0.47 +0.7 321,563 + Dshi_0334 0.47 +1.7 321,578 + Dshi_0334 0.48 +1.3 321,748 + Dshi_0334 0.61 -0.2 321,962 - Dshi_0334 0.76 +0.4 321,985 - Dshi_0334 0.77 +0.2 322,001 + Dshi_0334 0.79 -0.5 322,001 + Dshi_0334 0.79 -0.3 322,001 + Dshi_0334 0.79 +1.0 322,009 - Dshi_0334 0.79 +0.1 322,029 + Dshi_0334 0.81 -0.2 322,078 + Dshi_0334 0.84 +0.0 322,080 + Dshi_0334 0.84 +1.1 322,162 - -1.3 322,334 - +1.0 322,381 + -0.2 322,439 + -0.5 322,439 + +1.1 322,439 + +0.1 322,447 - +0.7 322,447 - -1.4 322,447 - -0.8 322,512 - Dshi_0335 0.12 +0.6 322,589 + Dshi_0335 0.18 -0.4 322,708 + Dshi_0335 0.26 -0.3 322,716 - Dshi_0335 0.27 +0.6 322,742 - Dshi_0335 0.29 -1.6 322,807 + Dshi_0335 0.33 -1.9 323,157 - Dshi_0335 0.58 -0.0 323,165 - Dshi_0335 0.59 -0.5 323,173 - Dshi_0335 0.59 -0.7 323,187 + Dshi_0335 0.60 -0.0 323,263 - Dshi_0335 0.65 -0.9 323,263 - Dshi_0335 0.65 +0.0 323,263 - Dshi_0335 0.65 +0.8 323,263 - Dshi_0335 0.65 +0.2
Or see this region's nucleotide sequence