Experiment: m.b. Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0981 and Dshi_0982 are separated by 149 nucleotides Dshi_0982 and Dshi_0983 are separated by 2 nucleotides Dshi_0983 and Dshi_0984 overlap by 4 nucleotides Dshi_0984 and Dshi_R0023 are separated by 292 nucleotides Dshi_R0023 and Dshi_0985 are separated by 423 nucleotides
Dshi_0981: Dshi_0981 - protein tyrosine phosphatase (RefSeq), at 1,002,333 to 1,002,788
_0981
Dshi_0982: Dshi_0982 - translation initiation factor IF-1 (RefSeq), at 1,002,938 to 1,003,156
_0982
Dshi_0983: Dshi_0983 - maf protein (RefSeq), at 1,003,159 to 1,003,743
_0983
Dshi_0984: Dshi_0984 - ribonuclease (RefSeq), at 1,003,740 to 1,004,783
_0984
Dshi_R0023: Dshi_R0023 - tRNA-Phe (RefSeq), at 1,005,076 to 1,005,150
_R0023
Dshi_0985: Dshi_0985 - hypothetical protein (RefSeq), at 1,005,574 to 1,005,831
_0985
Position (kb)
1003
1004
1005 Strain fitness (log2 ratio)
-2
-1
0
1 at 1003.232 kb on - strand, within Dshi_0983 at 1003.381 kb on + strand, within Dshi_0983 at 1003.496 kb on + strand, within Dshi_0983 at 1003.531 kb on + strand, within Dshi_0983 at 1003.602 kb on + strand, within Dshi_0983 at 1003.658 kb on - strand, within Dshi_0983 at 1003.680 kb on - strand, within Dshi_0983 at 1003.715 kb on + strand at 1003.715 kb on + strand at 1003.991 kb on + strand, within Dshi_0984 at 1004.045 kb on + strand, within Dshi_0984 at 1004.191 kb on + strand, within Dshi_0984 at 1004.260 kb on - strand, within Dshi_0984 at 1004.260 kb on - strand, within Dshi_0984 at 1004.260 kb on - strand, within Dshi_0984 at 1004.297 kb on + strand, within Dshi_0984 at 1004.431 kb on + strand, within Dshi_0984 at 1004.444 kb on + strand, within Dshi_0984 at 1004.444 kb on + strand, within Dshi_0984 at 1004.484 kb on - strand, within Dshi_0984 at 1004.598 kb on + strand, within Dshi_0984 at 1004.598 kb on - strand, within Dshi_0984 at 1004.693 kb on + strand at 1004.715 kb on + strand at 1004.716 kb on + strand at 1004.725 kb on + strand at 1004.785 kb on - strand at 1004.787 kb on + strand at 1004.818 kb on - strand at 1004.863 kb on - strand at 1004.928 kb on - strand at 1004.980 kb on + strand at 1005.318 kb on - strand at 1005.322 kb on + strand at 1005.358 kb on - strand at 1005.574 kb on - strand at 1005.593 kb on - strand at 1005.596 kb on + strand at 1005.695 kb on + strand, within Dshi_0985 at 1005.731 kb on - strand, within Dshi_0985 at 1005.731 kb on - strand, within Dshi_0985 at 1005.743 kb on + strand, within Dshi_0985 at 1005.748 kb on + strand, within Dshi_0985 at 1005.748 kb on + strand, within Dshi_0985
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nickel (II) chloride 1 mM remove 1,003,232 - Dshi_0983 0.12 +0.3 1,003,381 + Dshi_0983 0.38 -0.5 1,003,496 + Dshi_0983 0.58 +0.1 1,003,531 + Dshi_0983 0.64 +0.4 1,003,602 + Dshi_0983 0.76 +0.8 1,003,658 - Dshi_0983 0.85 +0.1 1,003,680 - Dshi_0983 0.89 -0.8 1,003,715 + +0.1 1,003,715 + -0.4 1,003,991 + Dshi_0984 0.24 -0.3 1,004,045 + Dshi_0984 0.29 -0.1 1,004,191 + Dshi_0984 0.43 -0.1 1,004,260 - Dshi_0984 0.50 +0.1 1,004,260 - Dshi_0984 0.50 -0.0 1,004,260 - Dshi_0984 0.50 -0.8 1,004,297 + Dshi_0984 0.53 -1.4 1,004,431 + Dshi_0984 0.66 +0.3 1,004,444 + Dshi_0984 0.67 -0.7 1,004,444 + Dshi_0984 0.67 +0.1 1,004,484 - Dshi_0984 0.71 +0.9 1,004,598 + Dshi_0984 0.82 +0.6 1,004,598 - Dshi_0984 0.82 -0.1 1,004,693 + -0.8 1,004,715 + +0.7 1,004,716 + -0.0 1,004,725 + +0.9 1,004,785 - -0.5 1,004,787 + +0.7 1,004,818 - -0.7 1,004,863 - -2.1 1,004,928 - +0.4 1,004,980 + +0.1 1,005,318 - -2.1 1,005,322 + -0.4 1,005,358 - -0.6 1,005,574 - +0.2 1,005,593 - -0.5 1,005,596 + -0.3 1,005,695 + Dshi_0985 0.47 -0.1 1,005,731 - Dshi_0985 0.61 -0.4 1,005,731 - Dshi_0985 0.61 -1.1 1,005,743 + Dshi_0985 0.66 +1.2 1,005,748 + Dshi_0985 0.67 +1.0 1,005,748 + Dshi_0985 0.67 +1.0
Or see this region's nucleotide sequence