Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2132 and Dshi_2133 overlap by 4 nucleotides Dshi_2133 and Dshi_2134 are separated by 209 nucleotides Dshi_2134 and Dshi_2135 are separated by 122 nucleotides Dshi_2135 and Dshi_2136 are separated by 99 nucleotides
Dshi_2132: Dshi_2132 - amidophosphoribosyltransferase (RefSeq), at 2,262,378 to 2,263,904
_2132
Dshi_2133: Dshi_2133 - protein of unknown function DUF1284 (RefSeq), at 2,263,901 to 2,264,419
_2133
Dshi_2134: Dshi_2134 - BioY protein (RefSeq), at 2,264,629 to 2,265,222
_2134
Dshi_2135: Dshi_2135 - short-chain dehydrogenase/reductase SDR (RefSeq), at 2,265,345 to 2,266,010
_2135
Dshi_2136: Dshi_2136 - stationary-phase survival protein SurE (RefSeq), at 2,266,110 to 2,266,895
_2136
Position (kb)
2264
2265
2266 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2263.637 kb on - strand, within Dshi_2132 at 2263.657 kb on + strand, within Dshi_2132 at 2263.722 kb on + strand, within Dshi_2132 at 2263.765 kb on - strand at 2263.863 kb on - strand at 2263.876 kb on + strand at 2263.942 kb on - strand at 2263.962 kb on - strand, within Dshi_2133 at 2264.056 kb on - strand, within Dshi_2133 at 2264.076 kb on + strand, within Dshi_2133 at 2264.178 kb on + strand, within Dshi_2133 at 2264.317 kb on - strand, within Dshi_2133 at 2264.449 kb on + strand at 2264.449 kb on + strand at 2264.569 kb on + strand at 2264.813 kb on + strand, within Dshi_2134 at 2265.205 kb on - strand at 2265.323 kb on - strand at 2265.329 kb on + strand at 2265.455 kb on + strand, within Dshi_2135 at 2266.051 kb on + strand at 2266.089 kb on + strand at 2266.101 kb on + strand at 2266.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,263,637 - Dshi_2132 0.82 -4.1 2,263,657 + Dshi_2132 0.84 -3.8 2,263,722 + Dshi_2132 0.88 -1.8 2,263,765 - -4.2 2,263,863 - -0.2 2,263,876 + -0.1 2,263,942 - -1.0 2,263,962 - Dshi_2133 0.12 +1.0 2,264,056 - Dshi_2133 0.30 -0.4 2,264,076 + Dshi_2133 0.34 -0.3 2,264,178 + Dshi_2133 0.53 -0.4 2,264,317 - Dshi_2133 0.80 -1.4 2,264,449 + +0.3 2,264,449 + -0.2 2,264,569 + +0.5 2,264,813 + Dshi_2134 0.31 -0.0 2,265,205 - +0.7 2,265,323 - -0.8 2,265,329 + -2.0 2,265,455 + Dshi_2135 0.17 -0.6 2,266,051 + +0.4 2,266,089 + -0.1 2,266,101 + -1.2 2,266,106 + +1.2
Or see this region's nucleotide sequence