Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1535 and Dshi_1536 are separated by 16 nucleotides Dshi_1536 and Dshi_1537 overlap by 1 nucleotides Dshi_1537 and Dshi_1538 are separated by 4 nucleotides Dshi_1538 and Dshi_1539 are separated by 157 nucleotides
Dshi_1535: Dshi_1535 - phosphate ABC transporter, inner membrane subunit PstA (RefSeq), at 1,596,265 to 1,597,617
_1535
Dshi_1536: Dshi_1536 - phosphate ABC transporter, ATPase subunit (RefSeq), at 1,597,634 to 1,598,428
_1536
Dshi_1537: Dshi_1537 - phosphate uptake regulator, PhoU (RefSeq), at 1,598,428 to 1,599,141
_1537
Dshi_1538: Dshi_1538 - two component transcriptional regulator, winged helix family (RefSeq), at 1,599,146 to 1,599,832
_1538
Dshi_1539: Dshi_1539 - Na+/Ca+ antiporter, CaCA family (RefSeq), at 1,599,990 to 1,600,946
_1539
Position (kb) 1598
1599
1600 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1598.883 kb on + strand, within Dshi_1537 at 1598.922 kb on + strand, within Dshi_1537 at 1598.947 kb on + strand, within Dshi_1537 at 1599.136 kb on + strand at 1599.887 kb on + strand at 1599.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,598,883 + Dshi_1537 0.64 -2.7 1,598,922 + Dshi_1537 0.69 -1.8 1,598,947 + Dshi_1537 0.73 -0.6 1,599,136 + +2.9 1,599,887 + +0.9 1,599,965 - -1.5
Or see this region's nucleotide sequence