Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2048 and Dshi_2049 are separated by 21 nucleotides Dshi_2049 and Dshi_2050 overlap by 1 nucleotides Dshi_2050 and Dshi_2051 are separated by 105 nucleotides Dshi_2051 and Dshi_2052 overlap by 4 nucleotides
Dshi_2048: Dshi_2048 - Mammalian cell entry related domain protein (RefSeq), at 2,167,769 to 2,169,508
_2048
Dshi_2049: Dshi_2049 - ABC transporter related (RefSeq), at 2,169,530 to 2,170,309
_2049
Dshi_2050: Dshi_2050 - protein of unknown function DUF140 (RefSeq), at 2,170,309 to 2,171,463
_2050
Dshi_2051: Dshi_2051 - hypothetical protein (RefSeq), at 2,171,569 to 2,172,045
_2051
Dshi_2052: Dshi_2052 - hypothetical protein (RefSeq), at 2,172,042 to 2,172,206
_2052
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2169.356 kb on + strand at 2169.370 kb on - strand at 2169.409 kb on - strand at 2169.411 kb on + strand at 2169.419 kb on - strand at 2169.419 kb on - strand at 2169.563 kb on - strand at 2169.596 kb on - strand at 2169.624 kb on - strand, within Dshi_2049 at 2169.687 kb on + strand, within Dshi_2049 at 2169.717 kb on - strand, within Dshi_2049 at 2169.749 kb on - strand, within Dshi_2049 at 2169.870 kb on - strand, within Dshi_2049 at 2169.958 kb on + strand, within Dshi_2049 at 2169.980 kb on + strand, within Dshi_2049 at 2169.980 kb on + strand, within Dshi_2049 at 2169.988 kb on - strand, within Dshi_2049 at 2170.014 kb on - strand, within Dshi_2049 at 2170.093 kb on + strand, within Dshi_2049 at 2170.204 kb on - strand, within Dshi_2049 at 2170.386 kb on - strand at 2170.433 kb on - strand, within Dshi_2050 at 2170.457 kb on + strand, within Dshi_2050 at 2170.490 kb on + strand, within Dshi_2050 at 2170.490 kb on + strand, within Dshi_2050 at 2170.591 kb on - strand, within Dshi_2050 at 2170.636 kb on + strand, within Dshi_2050 at 2170.758 kb on - strand, within Dshi_2050 at 2170.793 kb on + strand, within Dshi_2050 at 2170.924 kb on + strand, within Dshi_2050 at 2170.931 kb on + strand, within Dshi_2050 at 2170.931 kb on + strand, within Dshi_2050 at 2171.007 kb on - strand, within Dshi_2050 at 2171.017 kb on - strand, within Dshi_2050 at 2171.017 kb on - strand, within Dshi_2050 at 2171.073 kb on - strand, within Dshi_2050 at 2171.119 kb on - strand, within Dshi_2050 at 2171.240 kb on + strand, within Dshi_2050 at 2171.272 kb on - strand, within Dshi_2050 at 2171.274 kb on + strand, within Dshi_2050 at 2171.479 kb on - strand at 2171.479 kb on - strand at 2171.503 kb on - strand at 2171.508 kb on - strand at 2171.532 kb on - strand at 2171.541 kb on + strand at 2171.549 kb on - strand at 2171.739 kb on - strand, within Dshi_2051 at 2171.762 kb on + strand, within Dshi_2051 at 2171.775 kb on - strand, within Dshi_2051 at 2171.798 kb on + strand, within Dshi_2051 at 2171.802 kb on - strand, within Dshi_2051 at 2171.843 kb on - strand, within Dshi_2051 at 2171.863 kb on + strand, within Dshi_2051 at 2171.889 kb on + strand, within Dshi_2051 at 2171.891 kb on - strand, within Dshi_2051 at 2171.912 kb on + strand, within Dshi_2051 at 2172.085 kb on + strand, within Dshi_2052 at 2172.103 kb on + strand, within Dshi_2052 at 2172.204 kb on - strand at 2172.211 kb on - strand at 2172.262 kb on + strand at 2172.317 kb on + strand at 2172.335 kb on - strand at 2172.368 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 2,169,356 + +1.3 2,169,370 - -0.3 2,169,409 - +0.9 2,169,411 + +0.6 2,169,419 - -0.1 2,169,419 - -0.6 2,169,563 - -0.9 2,169,596 - -0.3 2,169,624 - Dshi_2049 0.12 +0.4 2,169,687 + Dshi_2049 0.20 +0.2 2,169,717 - Dshi_2049 0.24 +0.7 2,169,749 - Dshi_2049 0.28 +0.1 2,169,870 - Dshi_2049 0.44 -1.1 2,169,958 + Dshi_2049 0.55 -0.8 2,169,980 + Dshi_2049 0.58 -0.2 2,169,980 + Dshi_2049 0.58 +0.3 2,169,988 - Dshi_2049 0.59 -0.1 2,170,014 - Dshi_2049 0.62 -1.2 2,170,093 + Dshi_2049 0.72 -1.1 2,170,204 - Dshi_2049 0.86 -1.0 2,170,386 - -0.4 2,170,433 - Dshi_2050 0.11 -1.1 2,170,457 + Dshi_2050 0.13 +0.4 2,170,490 + Dshi_2050 0.16 -0.1 2,170,490 + Dshi_2050 0.16 -0.9 2,170,591 - Dshi_2050 0.24 -1.5 2,170,636 + Dshi_2050 0.28 +0.3 2,170,758 - Dshi_2050 0.39 +0.4 2,170,793 + Dshi_2050 0.42 +0.6 2,170,924 + Dshi_2050 0.53 +0.3 2,170,931 + Dshi_2050 0.54 -0.2 2,170,931 + Dshi_2050 0.54 -0.1 2,171,007 - Dshi_2050 0.60 -0.4 2,171,017 - Dshi_2050 0.61 -1.2 2,171,017 - Dshi_2050 0.61 +0.2 2,171,073 - Dshi_2050 0.66 +0.0 2,171,119 - Dshi_2050 0.70 -0.4 2,171,240 + Dshi_2050 0.81 -0.8 2,171,272 - Dshi_2050 0.83 -0.8 2,171,274 + Dshi_2050 0.84 +0.1 2,171,479 - -1.1 2,171,479 - +0.6 2,171,503 - +0.6 2,171,508 - +0.0 2,171,532 - +0.2 2,171,541 + -1.1 2,171,549 - -0.9 2,171,739 - Dshi_2051 0.36 -0.6 2,171,762 + Dshi_2051 0.40 -0.5 2,171,775 - Dshi_2051 0.43 +1.3 2,171,798 + Dshi_2051 0.48 -0.9 2,171,802 - Dshi_2051 0.49 -2.0 2,171,843 - Dshi_2051 0.57 -1.0 2,171,863 + Dshi_2051 0.62 -3.0 2,171,889 + Dshi_2051 0.67 -0.6 2,171,891 - Dshi_2051 0.68 +0.4 2,171,912 + Dshi_2051 0.72 -2.0 2,172,085 + Dshi_2052 0.26 -0.6 2,172,103 + Dshi_2052 0.37 -2.6 2,172,204 - +0.5 2,172,211 - -0.9 2,172,262 + -1.9 2,172,317 + -1.1 2,172,335 - -0.2 2,172,368 - -1.9
Or see this region's nucleotide sequence