Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0253 and Dshi_0254 are separated by 30 nucleotides Dshi_0254 and Dshi_0255 are separated by 156 nucleotides Dshi_0255 and Dshi_0256 are separated by 78 nucleotides Dshi_0256 and Dshi_0257 are separated by 59 nucleotides
Dshi_0253: Dshi_0253 - AMP-dependent synthetase and ligase (RefSeq), at 251,200 to 252,759
_0253
Dshi_0254: Dshi_0254 - ribonuclease T2 (RefSeq), at 252,790 to 253,431
_0254
Dshi_0255: Dshi_0255 - protein of unknown function DUF1013 (RefSeq), at 253,588 to 254,340
_0255
Dshi_0256: Dshi_0256 - hypothetical protein (RefSeq), at 254,419 to 254,667
_0256
Dshi_0257: Dshi_0257 - peptidase S8 and S53 subtilisin kexin sedolisin (RefSeq), at 254,727 to 256,076
_0257
Position (kb) 253
254
255 Strain fitness (log2 ratio) -1
0
1
2 at 252.617 kb on + strand at 252.668 kb on + strand at 252.737 kb on - strand at 252.748 kb on + strand at 252.793 kb on + strand at 252.796 kb on - strand at 252.869 kb on - strand, within Dshi_0254 at 252.878 kb on + strand, within Dshi_0254 at 252.892 kb on + strand, within Dshi_0254 at 252.939 kb on - strand, within Dshi_0254 at 253.305 kb on + strand, within Dshi_0254 at 253.370 kb on - strand at 253.388 kb on - strand at 253.416 kb on - strand at 253.419 kb on - strand at 254.171 kb on + strand, within Dshi_0255 at 254.320 kb on - strand at 254.413 kb on - strand at 254.687 kb on - strand at 254.799 kb on - strand at 254.873 kb on - strand, within Dshi_0257 at 254.955 kb on + strand, within Dshi_0257 at 255.010 kb on - strand, within Dshi_0257 at 255.017 kb on - strand, within Dshi_0257 at 255.088 kb on + strand, within Dshi_0257 at 255.231 kb on - strand, within Dshi_0257 at 255.244 kb on + strand, within Dshi_0257 at 255.306 kb on + strand, within Dshi_0257
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 252,617 + +0.5 252,668 + -1.0 252,737 - -0.7 252,748 + -0.2 252,793 + +1.0 252,796 - +0.8 252,869 - Dshi_0254 0.12 +0.7 252,878 + Dshi_0254 0.14 -1.6 252,892 + Dshi_0254 0.16 +0.3 252,939 - Dshi_0254 0.23 -0.8 253,305 + Dshi_0254 0.80 +0.5 253,370 - +0.3 253,388 - +0.1 253,416 - -0.5 253,419 - +2.0 254,171 + Dshi_0255 0.77 -0.9 254,320 - +0.4 254,413 - -0.3 254,687 - -0.6 254,799 - +0.9 254,873 - Dshi_0257 0.11 +1.4 254,955 + Dshi_0257 0.17 +0.4 255,010 - Dshi_0257 0.21 -0.6 255,017 - Dshi_0257 0.21 +0.3 255,088 + Dshi_0257 0.27 -0.2 255,231 - Dshi_0257 0.37 -1.0 255,244 + Dshi_0257 0.38 +0.1 255,306 + Dshi_0257 0.43 +0.0
Or see this region's nucleotide sequence