Experiment: Chlorate 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1517 and Psest_1518 are separated by 132 nucleotides Psest_1518 and Psest_1519 are separated by 166 nucleotides Psest_1519 and Psest_1520 are separated by 70 nucleotides Psest_1520 and Psest_1521 are separated by 56 nucleotides Psest_1521 and Psest_1522 are separated by 8 nucleotides
Psest_1517: Psest_1517 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,616,132 to 1,617,001
_1517
Psest_1518: Psest_1518 - tRNA_Ser_CGA, at 1,617,134 to 1,617,223
_1518
Psest_1519: Psest_1519 - DNA polymerase elongation subunit (family B), at 1,617,390 to 1,619,765
_1519
Psest_1520: Psest_1520 - hypothetical protein, at 1,619,836 to 1,620,144
_1520
Psest_1521: Psest_1521 - Uncharacterized protein conserved in bacteria, at 1,620,201 to 1,620,545
_1521
Psest_1522: Psest_1522 - hypothetical protein, at 1,620,554 to 1,620,745
_1522
Position (kb)
1617
1618
1619
1620 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1616.983 kb on + strand at 1616.983 kb on + strand at 1617.118 kb on + strand at 1617.181 kb on - strand, within Psest_1518 at 1617.196 kb on + strand, within Psest_1518 at 1617.196 kb on + strand, within Psest_1518 at 1617.197 kb on - strand, within Psest_1518 at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.225 kb on + strand at 1617.226 kb on - strand at 1617.226 kb on - strand at 1617.226 kb on - strand at 1617.226 kb on - strand at 1617.226 kb on - strand at 1617.226 kb on - strand at 1617.226 kb on - strand at 1617.226 kb on - strand at 1617.231 kb on + strand at 1617.232 kb on - strand at 1617.232 kb on - strand at 1617.232 kb on - strand at 1617.427 kb on + strand at 1617.428 kb on - strand at 1617.449 kb on + strand at 1617.652 kb on - strand, within Psest_1519 at 1617.652 kb on - strand, within Psest_1519 at 1617.903 kb on + strand, within Psest_1519 at 1617.903 kb on + strand, within Psest_1519 at 1617.903 kb on + strand, within Psest_1519 at 1617.904 kb on - strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.942 kb on + strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.943 kb on - strand, within Psest_1519 at 1617.944 kb on + strand, within Psest_1519 at 1617.944 kb on + strand, within Psest_1519 at 1617.944 kb on + strand, within Psest_1519 at 1617.944 kb on + strand, within Psest_1519 at 1617.944 kb on + strand, within Psest_1519 at 1617.944 kb on + strand, within Psest_1519 at 1617.996 kb on + strand, within Psest_1519 at 1617.996 kb on + strand, within Psest_1519 at 1618.398 kb on + strand, within Psest_1519 at 1618.399 kb on - strand, within Psest_1519 at 1618.536 kb on + strand, within Psest_1519 at 1618.536 kb on + strand, within Psest_1519 at 1618.536 kb on + strand, within Psest_1519 at 1618.536 kb on + strand, within Psest_1519 at 1618.536 kb on + strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.537 kb on - strand, within Psest_1519 at 1618.641 kb on + strand, within Psest_1519 at 1618.641 kb on + strand, within Psest_1519 at 1618.642 kb on - strand, within Psest_1519 at 1618.642 kb on - strand, within Psest_1519 at 1618.905 kb on + strand, within Psest_1519 at 1618.906 kb on - strand, within Psest_1519 at 1618.906 kb on - strand, within Psest_1519 at 1618.906 kb on - strand, within Psest_1519 at 1618.906 kb on - strand, within Psest_1519 at 1618.961 kb on + strand, within Psest_1519 at 1619.034 kb on + strand, within Psest_1519 at 1619.034 kb on + strand, within Psest_1519 at 1619.034 kb on + strand, within Psest_1519 at 1619.035 kb on - strand, within Psest_1519 at 1619.035 kb on - strand, within Psest_1519 at 1619.035 kb on - strand, within Psest_1519 at 1619.051 kb on + strand, within Psest_1519 at 1619.051 kb on + strand, within Psest_1519 at 1619.051 kb on + strand, within Psest_1519 at 1619.052 kb on - strand, within Psest_1519 at 1619.052 kb on - strand, within Psest_1519 at 1619.370 kb on + strand, within Psest_1519 at 1619.371 kb on - strand, within Psest_1519 at 1619.406 kb on + strand, within Psest_1519 at 1619.407 kb on - strand, within Psest_1519 at 1619.407 kb on - strand, within Psest_1519 at 1619.463 kb on + strand, within Psest_1519 at 1619.463 kb on + strand, within Psest_1519 at 1619.464 kb on - strand, within Psest_1519 at 1619.469 kb on - strand, within Psest_1519 at 1619.578 kb on - strand at 1619.601 kb on + strand at 1619.601 kb on + strand at 1619.601 kb on + strand at 1619.667 kb on + strand at 1619.667 kb on + strand at 1619.668 kb on - strand at 1619.668 kb on - strand at 1619.668 kb on - strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.835 kb on + strand at 1619.836 kb on - strand at 1619.836 kb on - strand at 1619.836 kb on - strand at 1619.866 kb on + strand at 1619.867 kb on - strand, within Psest_1520 at 1619.908 kb on - strand, within Psest_1520 at 1619.953 kb on + strand, within Psest_1520 at 1619.953 kb on + strand, within Psest_1520 at 1620.025 kb on + strand, within Psest_1520 at 1620.025 kb on + strand, within Psest_1520 at 1620.025 kb on + strand, within Psest_1520 at 1620.026 kb on - strand, within Psest_1520 at 1620.070 kb on + strand, within Psest_1520 at 1620.142 kb on + strand at 1620.142 kb on + strand at 1620.142 kb on + strand at 1620.142 kb on + strand at 1620.143 kb on - strand at 1620.143 kb on - strand at 1620.378 kb on - strand, within Psest_1521 at 1620.535 kb on + strand at 1620.536 kb on - strand at 1620.536 kb on - strand at 1620.612 kb on + strand, within Psest_1522 at 1620.612 kb on + strand, within Psest_1522 at 1620.612 kb on + strand, within Psest_1522 at 1620.612 kb on + strand, within Psest_1522 at 1620.689 kb on - strand, within Psest_1522
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorate 75 mM remove 1,616,983 + -1.1 1,616,983 + -2.2 1,617,118 + -1.1 1,617,181 - Psest_1518 0.52 -1.8 1,617,196 + Psest_1518 0.69 -0.7 1,617,196 + Psest_1518 0.69 -0.1 1,617,197 - Psest_1518 0.70 -1.7 1,617,225 + -1.9 1,617,225 + -0.7 1,617,225 + +1.6 1,617,225 + -1.4 1,617,225 + -2.1 1,617,225 + -3.3 1,617,225 + +0.2 1,617,225 + -0.2 1,617,225 + +1.6 1,617,226 - +0.5 1,617,226 - +1.6 1,617,226 - -0.5 1,617,226 - +0.1 1,617,226 - +0.4 1,617,226 - -1.8 1,617,226 - -0.1 1,617,226 - -1.9 1,617,231 + +1.2 1,617,232 - -1.9 1,617,232 - -0.3 1,617,232 - -0.1 1,617,427 + -1.7 1,617,428 - +0.3 1,617,449 + -1.9 1,617,652 - Psest_1519 0.11 +0.6 1,617,652 - Psest_1519 0.11 -4.0 1,617,903 + Psest_1519 0.22 +1.2 1,617,903 + Psest_1519 0.22 -3.9 1,617,903 + Psest_1519 0.22 -1.2 1,617,904 - Psest_1519 0.22 +0.4 1,617,942 + Psest_1519 0.23 -0.1 1,617,942 + Psest_1519 0.23 -1.2 1,617,942 + Psest_1519 0.23 +0.7 1,617,942 + Psest_1519 0.23 -0.0 1,617,942 + Psest_1519 0.23 -0.6 1,617,942 + Psest_1519 0.23 -1.1 1,617,942 + Psest_1519 0.23 -2.5 1,617,942 + Psest_1519 0.23 +0.3 1,617,942 + Psest_1519 0.23 -1.5 1,617,942 + Psest_1519 0.23 -1.4 1,617,943 - Psest_1519 0.23 -1.6 1,617,943 - Psest_1519 0.23 -1.0 1,617,943 - Psest_1519 0.23 +0.1 1,617,943 - Psest_1519 0.23 -1.1 1,617,943 - Psest_1519 0.23 -0.9 1,617,943 - Psest_1519 0.23 -0.1 1,617,943 - Psest_1519 0.23 -2.1 1,617,943 - Psest_1519 0.23 -0.3 1,617,943 - Psest_1519 0.23 -1.6 1,617,944 + Psest_1519 0.23 +0.6 1,617,944 + Psest_1519 0.23 -1.2 1,617,944 + Psest_1519 0.23 +0.5 1,617,944 + Psest_1519 0.23 +0.1 1,617,944 + Psest_1519 0.23 -0.4 1,617,944 + Psest_1519 0.23 -1.8 1,617,996 + Psest_1519 0.26 +0.1 1,617,996 + Psest_1519 0.26 -1.0 1,618,398 + Psest_1519 0.42 -2.6 1,618,399 - Psest_1519 0.42 -0.7 1,618,536 + Psest_1519 0.48 +1.3 1,618,536 + Psest_1519 0.48 +0.5 1,618,536 + Psest_1519 0.48 +0.1 1,618,536 + Psest_1519 0.48 +0.7 1,618,536 + Psest_1519 0.48 +2.1 1,618,537 - Psest_1519 0.48 +3.2 1,618,537 - Psest_1519 0.48 -0.1 1,618,537 - Psest_1519 0.48 -0.2 1,618,537 - Psest_1519 0.48 -1.8 1,618,537 - Psest_1519 0.48 -2.7 1,618,537 - Psest_1519 0.48 -2.1 1,618,537 - Psest_1519 0.48 +0.8 1,618,537 - Psest_1519 0.48 -0.2 1,618,537 - Psest_1519 0.48 -1.7 1,618,641 + Psest_1519 0.53 -0.5 1,618,641 + Psest_1519 0.53 -1.3 1,618,642 - Psest_1519 0.53 +2.3 1,618,642 - Psest_1519 0.53 -3.0 1,618,905 + Psest_1519 0.64 -0.2 1,618,906 - Psest_1519 0.64 -2.5 1,618,906 - Psest_1519 0.64 +1.5 1,618,906 - Psest_1519 0.64 -2.6 1,618,906 - Psest_1519 0.64 -0.6 1,618,961 + Psest_1519 0.66 +2.5 1,619,034 + Psest_1519 0.69 -1.0 1,619,034 + Psest_1519 0.69 -1.8 1,619,034 + Psest_1519 0.69 +3.0 1,619,035 - Psest_1519 0.69 -1.7 1,619,035 - Psest_1519 0.69 -2.5 1,619,035 - Psest_1519 0.69 -1.3 1,619,051 + Psest_1519 0.70 -0.6 1,619,051 + Psest_1519 0.70 -1.3 1,619,051 + Psest_1519 0.70 -0.0 1,619,052 - Psest_1519 0.70 -0.5 1,619,052 - Psest_1519 0.70 -2.4 1,619,370 + Psest_1519 0.83 -2.2 1,619,371 - Psest_1519 0.83 -1.5 1,619,406 + Psest_1519 0.85 -1.5 1,619,407 - Psest_1519 0.85 -1.1 1,619,407 - Psest_1519 0.85 -1.5 1,619,463 + Psest_1519 0.87 +0.8 1,619,463 + Psest_1519 0.87 -1.8 1,619,464 - Psest_1519 0.87 -0.0 1,619,469 - Psest_1519 0.88 -1.1 1,619,578 - -0.1 1,619,601 + -2.2 1,619,601 + +1.3 1,619,601 + -0.2 1,619,667 + -1.4 1,619,667 + +2.3 1,619,668 - -0.5 1,619,668 - -2.1 1,619,668 - -2.5 1,619,835 + -4.5 1,619,835 + -0.9 1,619,835 + -1.9 1,619,835 + +2.3 1,619,835 + -1.9 1,619,835 + +0.5 1,619,835 + -1.5 1,619,836 - +3.0 1,619,836 - +0.3 1,619,836 - -2.0 1,619,866 + -1.6 1,619,867 - Psest_1520 0.10 +0.5 1,619,908 - Psest_1520 0.23 -1.7 1,619,953 + Psest_1520 0.38 -0.5 1,619,953 + Psest_1520 0.38 +1.3 1,620,025 + Psest_1520 0.61 +1.2 1,620,025 + Psest_1520 0.61 -0.7 1,620,025 + Psest_1520 0.61 -1.7 1,620,026 - Psest_1520 0.61 -0.0 1,620,070 + Psest_1520 0.76 -1.8 1,620,142 + -1.4 1,620,142 + -1.8 1,620,142 + -0.3 1,620,142 + -0.2 1,620,143 - -1.8 1,620,143 - -1.8 1,620,378 - Psest_1521 0.51 +0.8 1,620,535 + -0.0 1,620,536 - -0.6 1,620,536 - +0.4 1,620,612 + Psest_1522 0.30 +1.5 1,620,612 + Psest_1522 0.30 -1.5 1,620,612 + Psest_1522 0.30 -0.4 1,620,612 + Psest_1522 0.30 -0.4 1,620,689 - Psest_1522 0.70 -2.7
Or see this region's nucleotide sequence