Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsA and ihfB are separated by 76 nucleotides ihfB and Sama_1738 are separated by 82 nucleotides Sama_1738 and Sama_1739 are separated by 1 nucleotides Sama_1739 and Sama_1740 are separated by 27 nucleotides Sama_1740 and Sama_1741 are separated by 64 nucleotides
Sama_1736: rpsA - 30S ribosomal protein S1 (RefSeq), at 2,126,185 to 2,127,852
rpsA
Sama_1737: ihfB - integration host factor subunit beta (RefSeq), at 2,127,929 to 2,128,216
ihfB
Sama_1738: Sama_1738 - membrane protein (RefSeq), at 2,128,299 to 2,128,640
_1738
Sama_1739: Sama_1739 - tetratricopeptide repeat protein (RefSeq), at 2,128,642 to 2,129,802
_1739
Sama_1740: Sama_1740 - orotidine 5'-phosphate decarboxylase (RefSeq), at 2,129,830 to 2,130,531
_1740
Sama_1741: Sama_1741 - short chain dehydrogenase (RefSeq), at 2,130,596 to 2,131,414
_1741
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2127.912 kb on + strand at 2127.915 kb on + strand at 2127.921 kb on + strand at 2127.921 kb on + strand at 2127.925 kb on + strand at 2127.993 kb on + strand, within ihfB at 2128.033 kb on + strand, within ihfB at 2128.091 kb on + strand, within ihfB at 2128.154 kb on + strand, within ihfB at 2128.521 kb on + strand, within Sama_1738 at 2129.806 kb on + strand at 2129.806 kb on + strand at 2129.876 kb on - strand at 2129.970 kb on - strand, within Sama_1740 at 2130.012 kb on + strand, within Sama_1740 at 2130.186 kb on + strand, within Sama_1740 at 2130.266 kb on + strand, within Sama_1740 at 2130.318 kb on + strand, within Sama_1740 at 2130.319 kb on - strand, within Sama_1740 at 2130.324 kb on + strand, within Sama_1740 at 2130.368 kb on + strand, within Sama_1740 at 2130.592 kb on + strand at 2130.592 kb on + strand at 2130.592 kb on + strand at 2130.736 kb on + strand, within Sama_1741 at 2130.765 kb on + strand, within Sama_1741
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 2,127,912 + -0.5 2,127,915 + +0.8 2,127,921 + +1.1 2,127,921 + -0.6 2,127,925 + +0.2 2,127,993 + ihfB Sama_1737 0.22 +0.7 2,128,033 + ihfB Sama_1737 0.36 +1.0 2,128,091 + ihfB Sama_1737 0.56 +2.9 2,128,154 + ihfB Sama_1737 0.78 +0.5 2,128,521 + Sama_1738 0.65 -1.9 2,129,806 + -2.2 2,129,806 + -0.3 2,129,876 - -0.8 2,129,970 - Sama_1740 0.20 -0.8 2,130,012 + Sama_1740 0.26 -0.3 2,130,186 + Sama_1740 0.51 -1.5 2,130,266 + Sama_1740 0.62 -0.9 2,130,318 + Sama_1740 0.70 -1.7 2,130,319 - Sama_1740 0.70 -2.6 2,130,324 + Sama_1740 0.70 -2.2 2,130,368 + Sama_1740 0.77 -2.7 2,130,592 + -0.5 2,130,592 + -1.4 2,130,592 + +0.4 2,130,736 + Sama_1741 0.17 -1.3 2,130,765 + Sama_1741 0.21 -1.3
Or see this region's nucleotide sequence