Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1230 and Sama_1231 are separated by 96 nucleotides Sama_1231 and Sama_1232 overlap by 20 nucleotides Sama_1232 and Sama_1233 are separated by 2 nucleotides
Sama_1230: Sama_1230 - trans-2-enoyl-CoA reductase (RefSeq), at 1,514,278 to 1,515,480
_1230
Sama_1231: Sama_1231 - peptide ABC transporter, ATP-binding protein (RefSeq), at 1,515,577 to 1,516,362
_1231
Sama_1232: Sama_1232 - peptide ABC transporter, ATP-binding protein (RefSeq), at 1,516,343 to 1,517,350
_1232
Sama_1233: Sama_1233 - peptide ABC transporter, permease protein (RefSeq), at 1,517,353 to 1,518,243
_1233
Position (kb)
1515
1516
1517 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1515.550 kb on - strand at 1515.626 kb on + strand at 1515.626 kb on + strand at 1515.626 kb on + strand at 1515.626 kb on + strand at 1515.753 kb on - strand, within Sama_1231 at 1515.753 kb on - strand, within Sama_1231 at 1515.781 kb on + strand, within Sama_1231 at 1516.045 kb on - strand, within Sama_1231 at 1516.084 kb on + strand, within Sama_1231 at 1516.085 kb on - strand, within Sama_1231 at 1516.200 kb on + strand, within Sama_1231 at 1516.201 kb on - strand, within Sama_1231 at 1516.225 kb on + strand, within Sama_1231 at 1516.225 kb on + strand, within Sama_1231 at 1516.226 kb on - strand, within Sama_1231 at 1516.226 kb on - strand, within Sama_1231 at 1516.286 kb on + strand at 1516.287 kb on - strand at 1516.363 kb on + strand at 1516.399 kb on - strand at 1516.401 kb on + strand at 1516.402 kb on - strand at 1516.402 kb on - strand at 1516.673 kb on - strand, within Sama_1232 at 1517.027 kb on + strand, within Sama_1232 at 1517.027 kb on + strand, within Sama_1232 at 1517.028 kb on - strand, within Sama_1232 at 1517.069 kb on + strand, within Sama_1232 at 1517.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 1,515,550 - -1.8 1,515,626 + -2.5 1,515,626 + -2.3 1,515,626 + -2.1 1,515,626 + -1.1 1,515,753 - Sama_1231 0.22 -1.6 1,515,753 - Sama_1231 0.22 -4.7 1,515,781 + Sama_1231 0.26 -0.7 1,516,045 - Sama_1231 0.60 -2.5 1,516,084 + Sama_1231 0.65 -0.7 1,516,085 - Sama_1231 0.65 -2.0 1,516,200 + Sama_1231 0.79 -0.7 1,516,201 - Sama_1231 0.79 -1.7 1,516,225 + Sama_1231 0.82 -0.2 1,516,225 + Sama_1231 0.82 -0.1 1,516,226 - Sama_1231 0.83 -2.8 1,516,226 - Sama_1231 0.83 -0.8 1,516,286 + -0.2 1,516,287 - -1.1 1,516,363 + -1.4 1,516,399 - -0.6 1,516,401 + -0.8 1,516,402 - -1.9 1,516,402 - -0.8 1,516,673 - Sama_1232 0.33 -2.1 1,517,027 + Sama_1232 0.68 -0.4 1,517,027 + Sama_1232 0.68 -0.6 1,517,028 - Sama_1232 0.68 -0.4 1,517,069 + Sama_1232 0.72 -0.7 1,517,351 - -1.3
Or see this region's nucleotide sequence