Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3959 and SO3960 overlap by 4 nucleotides SO3960 and rpoN are separated by 106 nucleotides rpoN and yfiA-3 are separated by 28 nucleotides yfiA-3 and ptsN are separated by 2 nucleotides ptsN and SO3964 overlap by 4 nucleotides
SO3959: SO3959 - conserved hypothetical protein (NCBI ptt file), at 4,100,717 to 4,101,268
SO3959
SO3960: SO3960 - ABC transporter, ATP-binding protein (NCBI ptt file), at 4,101,265 to 4,101,996
SO3960
SO3961: rpoN - RNA polymerase sigma-54 factor (NCBI ptt file), at 4,102,103 to 4,103,578
rpoN
SO3962: yfiA-3 - ribosomal subunit interface protein (NCBI ptt file), at 4,103,607 to 4,103,894
yfiA-3
SO3963: ptsN - nitrogen regulatory IIA protein (NCBI ptt file), at 4,103,897 to 4,104,340
ptsN
SO3964: SO3964 - conserved hypothetical protein (NCBI ptt file), at 4,104,337 to 4,105,191
SO3964
Position (kb) 4102
4103
4104 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 4102.018 kb on + strand at 4102.041 kb on - strand at 4102.057 kb on - strand at 4102.220 kb on + strand at 4102.221 kb on + strand at 4102.227 kb on + strand at 4102.229 kb on - strand at 4102.399 kb on + strand, within rpoN at 4102.438 kb on + strand, within rpoN at 4102.510 kb on - strand, within rpoN at 4102.645 kb on - strand, within rpoN at 4102.648 kb on + strand, within rpoN at 4102.678 kb on - strand, within rpoN at 4102.901 kb on - strand, within rpoN at 4102.936 kb on - strand, within rpoN at 4103.142 kb on + strand, within rpoN at 4103.157 kb on + strand, within rpoN at 4103.165 kb on - strand, within rpoN at 4103.197 kb on - strand, within rpoN at 4103.365 kb on + strand, within rpoN at 4103.365 kb on + strand, within rpoN at 4103.373 kb on - strand, within rpoN at 4103.373 kb on - strand, within rpoN at 4103.383 kb on - strand, within rpoN at 4103.389 kb on + strand, within rpoN at 4103.389 kb on + strand, within rpoN at 4103.397 kb on - strand, within rpoN at 4103.487 kb on - strand at 4103.535 kb on - strand at 4103.561 kb on - strand at 4103.563 kb on + strand at 4103.563 kb on + strand at 4103.642 kb on - strand, within yfiA-3 at 4103.657 kb on + strand, within yfiA-3 at 4103.705 kb on + strand, within yfiA-3 at 4103.823 kb on + strand, within yfiA-3 at 4103.895 kb on + strand at 4103.942 kb on + strand, within ptsN at 4103.942 kb on + strand, within ptsN at 4103.950 kb on - strand, within ptsN at 4103.993 kb on + strand, within ptsN at 4104.001 kb on - strand, within ptsN at 4104.065 kb on + strand, within ptsN at 4104.065 kb on + strand, within ptsN at 4104.108 kb on + strand, within ptsN at 4104.278 kb on + strand, within ptsN at 4104.386 kb on + strand at 4104.394 kb on - strand at 4104.394 kb on - strand at 4104.465 kb on + strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.574 kb on - strand, within SO3964
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 4,102,018 + +0.1 4,102,041 - +3.2 4,102,057 - +1.2 4,102,220 + +5.5 4,102,221 + +8.2 4,102,227 + +7.0 4,102,229 - +6.3 4,102,399 + rpoN SO3961 0.20 +6.7 4,102,438 + rpoN SO3961 0.23 +6.7 4,102,510 - rpoN SO3961 0.28 +5.7 4,102,645 - rpoN SO3961 0.37 +6.4 4,102,648 + rpoN SO3961 0.37 +6.7 4,102,678 - rpoN SO3961 0.39 +6.1 4,102,901 - rpoN SO3961 0.54 +6.6 4,102,936 - rpoN SO3961 0.56 +6.3 4,103,142 + rpoN SO3961 0.70 +5.4 4,103,157 + rpoN SO3961 0.71 +7.0 4,103,165 - rpoN SO3961 0.72 +6.8 4,103,197 - rpoN SO3961 0.74 +6.7 4,103,365 + rpoN SO3961 0.86 +6.6 4,103,365 + rpoN SO3961 0.86 +6.6 4,103,373 - rpoN SO3961 0.86 +6.0 4,103,373 - rpoN SO3961 0.86 +5.4 4,103,383 - rpoN SO3961 0.87 +6.4 4,103,389 + rpoN SO3961 0.87 +6.2 4,103,389 + rpoN SO3961 0.87 +7.0 4,103,397 - rpoN SO3961 0.88 +5.9 4,103,487 - +6.0 4,103,535 - +6.1 4,103,561 - +6.2 4,103,563 + -0.2 4,103,563 + +0.6 4,103,642 - yfiA-3 SO3962 0.12 -1.2 4,103,657 + yfiA-3 SO3962 0.17 -0.8 4,103,705 + yfiA-3 SO3962 0.34 +0.1 4,103,823 + yfiA-3 SO3962 0.75 -2.5 4,103,895 + -0.4 4,103,942 + ptsN SO3963 0.10 -2.3 4,103,942 + ptsN SO3963 0.10 -1.8 4,103,950 - ptsN SO3963 0.12 -2.0 4,103,993 + ptsN SO3963 0.22 -2.2 4,104,001 - ptsN SO3963 0.23 -1.0 4,104,065 + ptsN SO3963 0.38 -2.5 4,104,065 + ptsN SO3963 0.38 -2.0 4,104,108 + ptsN SO3963 0.48 -2.4 4,104,278 + ptsN SO3963 0.86 +1.9 4,104,386 + -0.7 4,104,394 - +0.0 4,104,394 - -0.6 4,104,465 + SO3964 0.15 -0.4 4,104,473 - SO3964 0.16 +0.8 4,104,473 - SO3964 0.16 -0.6 4,104,574 - SO3964 0.28 +1.2
Or see this region's nucleotide sequence