Strain Fitness in Shewanella oneidensis MR-1 around SO3530

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlytB and fkbP-2 are separated by 1 nucleotidesfkbP-2 and lspA overlap by 1 nucleotideslspA and ileS are separated by 9 nucleotides SO3529: lytB - penicillin tolerance protein LytB (NCBI ptt file), at 3,683,909 to 3,684,865 lytB SO3530: fkbP-2 - peptidyl-prolyl cis-trans isomerase FkbP (NCBI ptt file), at 3,684,867 to 3,685,286 fkbP-2 SO3531: lspA - lipoprotein signal peptidase (NCBI ptt file), at 3,685,286 to 3,685,798 lspA SO3532: ileS - isoleucyl-tRNA synthetase (NCBI ptt file), at 3,685,808 to 3,688,630 ileS Position (kb) 3684 3685 3686Strain fitness (log2 ratio) -2 -1 0 1at 3684.956 kb on - strand, within fkbP-2at 3685.083 kb on - strand, within fkbP-2at 3685.083 kb on - strand, within fkbP-2at 3685.117 kb on - strand, within fkbP-2at 3685.127 kb on - strand, within fkbP-2at 3685.208 kb on - strand, within fkbP-2

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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3,684,956 - fkbP-2 SO3530 0.21 -2.0
3,685,083 - fkbP-2 SO3530 0.51 -2.0
3,685,083 - fkbP-2 SO3530 0.51 -1.9
3,685,117 - fkbP-2 SO3530 0.60 -0.5
3,685,127 - fkbP-2 SO3530 0.62 -1.9
3,685,208 - fkbP-2 SO3530 0.81 -2.4

Or see this region's nucleotide sequence