Strain Fitness in Shewanella oneidensis MR-1 around SO3228

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliG and fliF overlap by 8 nucleotidesfliF and fliE are separated by 72 nucleotidesfliE and flrC are separated by 191 nucleotides SO3227: fliG - flagellar motor switch protein FliG (NCBI ptt file), at 3,365,531 to 3,366,577 fliG SO3228: fliF - flagellar M-ring protein FliF (NCBI ptt file), at 3,366,570 to 3,368,231 fliF SO3229: fliE - flagellar hook-basal body complex protein FliE (NCBI ptt file), at 3,368,304 to 3,368,642 fliE SO3230: flrC - flagellar regulatory protein C (NCBI ptt file), at 3,368,834 to 3,370,174 flrC Position (kb) 3366 3367 3368 3369Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7at 3365.583 kb on + strandat 3365.644 kb on - strand, within fliGat 3365.744 kb on - strand, within fliGat 3365.777 kb on - strand, within fliGat 3365.783 kb on + strand, within fliGat 3365.791 kb on - strand, within fliGat 3365.926 kb on - strand, within fliGat 3365.972 kb on + strand, within fliGat 3365.980 kb on - strand, within fliGat 3366.027 kb on + strand, within fliGat 3366.035 kb on - strand, within fliGat 3366.266 kb on + strand, within fliGat 3366.274 kb on - strand, within fliGat 3366.292 kb on - strand, within fliGat 3366.393 kb on - strand, within fliGat 3366.430 kb on - strand, within fliGat 3366.432 kb on + strand, within fliGat 3366.439 kb on + strand, within fliGat 3366.475 kb on + strandat 3366.646 kb on + strandat 3366.654 kb on - strandat 3366.694 kb on - strandat 3366.708 kb on + strandat 3366.716 kb on - strandat 3366.716 kb on - strandat 3366.737 kb on + strand, within fliFat 3366.737 kb on + strand, within fliFat 3366.744 kb on + strand, within fliFat 3366.745 kb on - strand, within fliFat 3366.745 kb on - strand, within fliFat 3366.745 kb on - strand, within fliFat 3366.764 kb on - strand, within fliFat 3366.849 kb on - strand, within fliFat 3366.988 kb on + strand, within fliFat 3367.003 kb on - strand, within fliFat 3367.030 kb on - strand, within fliFat 3367.081 kb on - strand, within fliFat 3367.123 kb on - strand, within fliFat 3367.123 kb on - strand, within fliFat 3367.186 kb on - strand, within fliFat 3367.622 kb on + strand, within fliFat 3367.629 kb on + strand, within fliFat 3367.637 kb on - strand, within fliFat 3367.682 kb on - strand, within fliFat 3367.712 kb on + strand, within fliFat 3367.846 kb on - strand, within fliFat 3367.856 kb on - strand, within fliFat 3367.879 kb on - strand, within fliFat 3367.923 kb on + strand, within fliFat 3367.931 kb on - strand, within fliFat 3367.944 kb on - strand, within fliFat 3368.039 kb on - strand, within fliFat 3368.081 kb on + strandat 3368.207 kb on - strandat 3368.479 kb on + strand, within fliEat 3368.487 kb on - strand, within fliEat 3368.651 kb on + strandat 3368.952 kb on - strandat 3368.979 kb on + strand, within flrCat 3368.979 kb on + strand, within flrCat 3369.028 kb on + strand, within flrCat 3369.083 kb on - strand, within flrCat 3369.093 kb on - strand, within flrCat 3369.123 kb on - strand, within flrCat 3369.128 kb on - strand, within flrCat 3369.155 kb on - strand, within flrC

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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3,365,583 + +6.2
3,365,644 - fliG SO3227 0.11 +6.3
3,365,744 - fliG SO3227 0.20 +7.0
3,365,777 - fliG SO3227 0.23 +6.4
3,365,783 + fliG SO3227 0.24 +7.0
3,365,791 - fliG SO3227 0.25 +5.2
3,365,926 - fliG SO3227 0.38 +5.8
3,365,972 + fliG SO3227 0.42 +6.5
3,365,980 - fliG SO3227 0.43 +6.7
3,366,027 + fliG SO3227 0.47 +6.5
3,366,035 - fliG SO3227 0.48 +6.3
3,366,266 + fliG SO3227 0.70 +6.9
3,366,274 - fliG SO3227 0.71 +7.7
3,366,292 - fliG SO3227 0.73 +6.8
3,366,393 - fliG SO3227 0.82 +6.4
3,366,430 - fliG SO3227 0.86 +5.7
3,366,432 + fliG SO3227 0.86 +6.6
3,366,439 + fliG SO3227 0.87 +6.5
3,366,475 + +6.5
3,366,646 + +6.2
3,366,654 - +6.7
3,366,694 - +6.1
3,366,708 + +7.1
3,366,716 - +6.2
3,366,716 - +6.1
3,366,737 + fliF SO3228 0.10 +7.2
3,366,737 + fliF SO3228 0.10 +6.9
3,366,744 + fliF SO3228 0.10 +6.7
3,366,745 - fliF SO3228 0.11 +6.0
3,366,745 - fliF SO3228 0.11 +6.6
3,366,745 - fliF SO3228 0.11 +7.3
3,366,764 - fliF SO3228 0.12 +5.9
3,366,849 - fliF SO3228 0.17 +6.1
3,366,988 + fliF SO3228 0.25 +6.9
3,367,003 - fliF SO3228 0.26 +6.3
3,367,030 - fliF SO3228 0.28 +6.3
3,367,081 - fliF SO3228 0.31 +6.7
3,367,123 - fliF SO3228 0.33 +5.8
3,367,123 - fliF SO3228 0.33 +6.5
3,367,186 - fliF SO3228 0.37 +6.6
3,367,622 + fliF SO3228 0.63 +6.7
3,367,629 + fliF SO3228 0.64 +5.7
3,367,637 - fliF SO3228 0.64 +6.3
3,367,682 - fliF SO3228 0.67 +6.1
3,367,712 + fliF SO3228 0.69 +7.1
3,367,846 - fliF SO3228 0.77 +6.5
3,367,856 - fliF SO3228 0.77 +6.3
3,367,879 - fliF SO3228 0.79 +6.5
3,367,923 + fliF SO3228 0.81 +6.8
3,367,931 - fliF SO3228 0.82 +6.3
3,367,944 - fliF SO3228 0.83 +6.9
3,368,039 - fliF SO3228 0.88 +6.0
3,368,081 + +6.5
3,368,207 - +6.6
3,368,479 + fliE SO3229 0.52 +5.7
3,368,487 - fliE SO3229 0.54 +5.4
3,368,651 + +7.2
3,368,952 - -0.0
3,368,979 + flrC SO3230 0.11 +1.0
3,368,979 + flrC SO3230 0.11 -0.0
3,369,028 + flrC SO3230 0.14 +0.3
3,369,083 - flrC SO3230 0.19 -0.5
3,369,093 - flrC SO3230 0.19 -0.1
3,369,123 - flrC SO3230 0.22 -0.3
3,369,128 - flrC SO3230 0.22 +0.8
3,369,155 - flrC SO3230 0.24 +0.9

Or see this region's nucleotide sequence