Strain Fitness in Shewanella oneidensis MR-1 around SO3221

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliP and fliO overlap by 1 nucleotidesfliO and fliN are separated by 34 nucleotidesfliN and fliM are separated by 12 nucleotidesfliM and fliL are separated by 39 nucleotidesfliL and fliK are separated by 82 nucleotides SO3218: fliP - flagellar biosynthetic protein FliP (NCBI ptt file), at 3,357,856 to 3,358,599 fliP SO3219: fliO - flagellar protein FliO (NCBI ptt file), at 3,358,599 to 3,359,006 fliO SO3220: fliN - flagellar motor switch protein FliN (NCBI ptt file), at 3,359,041 to 3,359,415 fliN SO3221: fliM - flagellar motor switch protein FliM (NCBI ptt file), at 3,359,428 to 3,360,459 fliM SO3222: fliL - flagellar protein FliL (NCBI ptt file), at 3,360,499 to 3,361,023 fliL SO3223: fliK - flagellar hook-length control protein FliK (NCBI ptt file), at 3,361,106 to 3,362,689 fliK Position (kb) 3359 3360 3361Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 3358.436 kb on - strand, within fliPat 3358.485 kb on - strand, within fliPat 3358.565 kb on + strandat 3358.594 kb on - strandat 3358.619 kb on + strandat 3358.757 kb on + strand, within fliOat 3358.757 kb on + strand, within fliOat 3358.861 kb on + strand, within fliOat 3358.962 kb on + strand, within fliOat 3359.028 kb on - strandat 3359.074 kb on + strandat 3359.082 kb on - strand, within fliNat 3359.157 kb on + strand, within fliNat 3359.178 kb on + strand, within fliNat 3359.180 kb on + strand, within fliNat 3359.299 kb on - strand, within fliNat 3359.300 kb on + strand, within fliNat 3359.327 kb on + strand, within fliNat 3359.336 kb on + strand, within fliNat 3359.401 kb on - strandat 3359.408 kb on + strandat 3359.518 kb on + strandat 3359.684 kb on - strand, within fliMat 3359.732 kb on - strand, within fliMat 3359.742 kb on - strand, within fliMat 3359.829 kb on - strand, within fliMat 3359.894 kb on + strand, within fliMat 3359.909 kb on + strand, within fliMat 3359.947 kb on + strand, within fliMat 3359.955 kb on - strand, within fliMat 3359.966 kb on + strand, within fliMat 3359.974 kb on - strand, within fliMat 3360.006 kb on - strand, within fliMat 3360.011 kb on - strand, within fliMat 3360.157 kb on + strand, within fliMat 3360.237 kb on - strand, within fliMat 3360.327 kb on + strand, within fliMat 3360.415 kb on + strandat 3360.426 kb on + strandat 3360.653 kb on - strand, within fliLat 3360.653 kb on - strand, within fliLat 3360.772 kb on - strand, within fliLat 3360.803 kb on - strand, within fliLat 3360.858 kb on - strand, within fliLat 3360.947 kb on - strand, within fliLat 3361.019 kb on + strandat 3361.024 kb on + strandat 3361.027 kb on - strandat 3361.058 kb on + strandat 3361.235 kb on + strandat 3361.251 kb on + strandat 3361.259 kb on - strandat 3361.259 kb on - strandat 3361.346 kb on + strand, within fliK

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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3,358,436 - fliP SO3218 0.78 +6.2
3,358,485 - fliP SO3218 0.85 +5.8
3,358,565 + +5.0
3,358,594 - +4.9
3,358,619 + +5.9
3,358,757 + fliO SO3219 0.39 +5.8
3,358,757 + fliO SO3219 0.39 +6.2
3,358,861 + fliO SO3219 0.64 +5.7
3,358,962 + fliO SO3219 0.89 +6.2
3,359,028 - +5.1
3,359,074 + +6.1
3,359,082 - fliN SO3220 0.11 +5.3
3,359,157 + fliN SO3220 0.31 +5.7
3,359,178 + fliN SO3220 0.37 -0.6
3,359,180 + fliN SO3220 0.37 +5.6
3,359,299 - fliN SO3220 0.69 +4.4
3,359,300 + fliN SO3220 0.69 +5.5
3,359,327 + fliN SO3220 0.76 +6.4
3,359,336 + fliN SO3220 0.79 +5.3
3,359,401 - +4.6
3,359,408 + +5.5
3,359,518 + +5.8
3,359,684 - fliM SO3221 0.25 +6.4
3,359,732 - fliM SO3221 0.29 +4.9
3,359,742 - fliM SO3221 0.30 +4.7
3,359,829 - fliM SO3221 0.39 +3.4
3,359,894 + fliM SO3221 0.45 +6.6
3,359,909 + fliM SO3221 0.47 +6.6
3,359,947 + fliM SO3221 0.50 +5.3
3,359,955 - fliM SO3221 0.51 +3.7
3,359,966 + fliM SO3221 0.52 +5.6
3,359,974 - fliM SO3221 0.53 +3.8
3,360,006 - fliM SO3221 0.56 +4.2
3,360,011 - fliM SO3221 0.56 +3.9
3,360,157 + fliM SO3221 0.71 +5.5
3,360,237 - fliM SO3221 0.78 +3.6
3,360,327 + fliM SO3221 0.87 +5.9
3,360,415 + +5.9
3,360,426 + +6.1
3,360,653 - fliL SO3222 0.29 +2.7
3,360,653 - fliL SO3222 0.29 +2.9
3,360,772 - fliL SO3222 0.52 +3.6
3,360,803 - fliL SO3222 0.58 +3.7
3,360,858 - fliL SO3222 0.68 +3.2
3,360,947 - fliL SO3222 0.85 +3.4
3,361,019 + +6.4
3,361,024 + +5.2
3,361,027 - +0.5
3,361,058 + +5.8
3,361,235 + +3.4
3,361,251 + +4.6
3,361,259 - +3.3
3,361,259 - +2.8
3,361,346 + fliK SO3223 0.15 +4.2

Or see this region's nucleotide sequence