Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpC/F and trpB are separated by 119 nucleotides trpB and trpA are separated by 5 nucleotides trpA and SO3025 are separated by 90 nucleotides
SO3022: trpC/F - indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (NCBI ptt file), at 3,133,337 to 3,134,746
trpC/F
SO3023: trpB - tryptophan synthase, beta subunit (NCBI ptt file), at 3,134,866 to 3,136,056
trpB
SO3024: trpA - tryptophan synthase, alpha subunit (NCBI ptt file), at 3,136,062 to 3,136,898
trpA
SO3025: SO3025 - conserved hypothetical protein (NCBI ptt file), at 3,136,989 to 3,137,981
SO3025
Position (kb)
3134
3135
3136
3137 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3133.945 kb on + strand, within trpC/F at 3134.058 kb on + strand, within trpC/F at 3134.139 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.257 kb on - strand, within trpC/F at 3134.345 kb on + strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.399 kb on - strand, within trpC/F at 3134.402 kb on + strand, within trpC/F at 3134.431 kb on - strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.496 kb on - strand, within trpC/F at 3134.552 kb on - strand, within trpC/F at 3134.703 kb on + strand at 3134.797 kb on + strand at 3134.850 kb on + strand at 3134.880 kb on - strand at 3134.996 kb on - strand, within trpB at 3134.996 kb on - strand, within trpB at 3135.032 kb on + strand, within trpB at 3135.111 kb on - strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.213 kb on - strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.234 kb on - strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.264 kb on + strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.398 kb on + strand, within trpB at 3135.624 kb on + strand, within trpB at 3135.651 kb on - strand, within trpB at 3135.659 kb on + strand, within trpB at 3135.775 kb on + strand, within trpB at 3135.775 kb on + strand, within trpB at 3135.783 kb on - strand, within trpB at 3135.805 kb on - strand, within trpB at 3135.805 kb on - strand, within trpB at 3135.933 kb on + strand, within trpB at 3135.941 kb on - strand at 3135.943 kb on - strand at 3136.061 kb on + strand at 3136.092 kb on + strand at 3136.150 kb on + strand, within trpA at 3136.160 kb on - strand, within trpA at 3136.183 kb on + strand, within trpA at 3136.191 kb on - strand, within trpA at 3136.191 kb on - strand, within trpA at 3136.218 kb on + strand, within trpA at 3136.242 kb on + strand, within trpA at 3136.339 kb on + strand, within trpA at 3136.390 kb on + strand, within trpA at 3136.390 kb on + strand, within trpA at 3136.409 kb on + strand, within trpA at 3136.417 kb on - strand, within trpA at 3136.417 kb on - strand, within trpA at 3136.530 kb on + strand, within trpA at 3136.532 kb on - strand, within trpA at 3136.532 kb on - strand, within trpA at 3136.538 kb on - strand, within trpA at 3136.610 kb on + strand, within trpA at 3136.618 kb on - strand, within trpA at 3136.722 kb on + strand, within trpA at 3136.757 kb on - strand, within trpA at 3136.757 kb on - strand, within trpA at 3136.987 kb on + strand at 3136.987 kb on + strand at 3137.004 kb on + strand at 3137.004 kb on + strand at 3137.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,133,945 + trpC/F SO3022 0.43 -1.7 3,134,058 + trpC/F SO3022 0.51 -3.4 3,134,139 + trpC/F SO3022 0.57 -1.3 3,134,249 + trpC/F SO3022 0.65 -3.2 3,134,249 + trpC/F SO3022 0.65 -2.3 3,134,257 - trpC/F SO3022 0.65 -3.4 3,134,345 + trpC/F SO3022 0.71 -4.6 3,134,365 - trpC/F SO3022 0.73 -3.3 3,134,365 - trpC/F SO3022 0.73 -4.8 3,134,399 - trpC/F SO3022 0.75 -5.3 3,134,402 + trpC/F SO3022 0.76 -3.4 3,134,431 - trpC/F SO3022 0.78 -1.9 3,134,454 + trpC/F SO3022 0.79 -3.9 3,134,454 + trpC/F SO3022 0.79 -1.7 3,134,496 - trpC/F SO3022 0.82 -4.6 3,134,552 - trpC/F SO3022 0.86 -2.0 3,134,703 + -1.3 3,134,797 + +0.3 3,134,850 + -0.7 3,134,880 - -4.4 3,134,996 - trpB SO3023 0.11 -3.7 3,134,996 - trpB SO3023 0.11 -2.6 3,135,032 + trpB SO3023 0.14 -4.5 3,135,111 - trpB SO3023 0.21 -3.0 3,135,139 + trpB SO3023 0.23 -3.2 3,135,139 + trpB SO3023 0.23 -1.2 3,135,205 + trpB SO3023 0.28 -4.6 3,135,205 + trpB SO3023 0.28 -1.7 3,135,213 - trpB SO3023 0.29 -3.6 3,135,226 + trpB SO3023 0.30 -2.8 3,135,226 + trpB SO3023 0.30 -0.0 3,135,234 - trpB SO3023 0.31 -1.7 3,135,262 + trpB SO3023 0.33 -3.4 3,135,262 + trpB SO3023 0.33 -1.7 3,135,262 + trpB SO3023 0.33 -3.7 3,135,264 + trpB SO3023 0.33 -2.7 3,135,270 - trpB SO3023 0.34 -3.7 3,135,270 - trpB SO3023 0.34 -0.9 3,135,270 - trpB SO3023 0.34 -4.0 3,135,270 - trpB SO3023 0.34 -2.9 3,135,272 - trpB SO3023 0.34 -4.8 3,135,272 - trpB SO3023 0.34 -4.2 3,135,398 + trpB SO3023 0.45 -5.0 3,135,624 + trpB SO3023 0.64 -2.5 3,135,651 - trpB SO3023 0.66 -5.6 3,135,659 + trpB SO3023 0.67 -4.6 3,135,775 + trpB SO3023 0.76 -2.6 3,135,775 + trpB SO3023 0.76 -4.5 3,135,783 - trpB SO3023 0.77 -1.7 3,135,805 - trpB SO3023 0.79 -3.4 3,135,805 - trpB SO3023 0.79 -2.5 3,135,933 + trpB SO3023 0.90 -2.0 3,135,941 - -3.9 3,135,943 - -0.9 3,136,061 + -0.7 3,136,092 + -2.5 3,136,150 + trpA SO3024 0.11 -2.9 3,136,160 - trpA SO3024 0.12 -2.7 3,136,183 + trpA SO3024 0.14 -2.3 3,136,191 - trpA SO3024 0.15 -3.2 3,136,191 - trpA SO3024 0.15 -3.8 3,136,218 + trpA SO3024 0.19 -2.4 3,136,242 + trpA SO3024 0.22 -1.8 3,136,339 + trpA SO3024 0.33 -2.0 3,136,390 + trpA SO3024 0.39 -2.3 3,136,390 + trpA SO3024 0.39 -3.2 3,136,409 + trpA SO3024 0.41 -2.9 3,136,417 - trpA SO3024 0.42 -2.7 3,136,417 - trpA SO3024 0.42 -3.9 3,136,530 + trpA SO3024 0.56 -3.5 3,136,532 - trpA SO3024 0.56 -1.5 3,136,532 - trpA SO3024 0.56 -4.5 3,136,538 - trpA SO3024 0.57 -2.3 3,136,610 + trpA SO3024 0.65 -3.4 3,136,618 - trpA SO3024 0.66 -1.7 3,136,722 + trpA SO3024 0.79 -3.8 3,136,757 - trpA SO3024 0.83 -3.9 3,136,757 - trpA SO3024 0.83 -1.3 3,136,987 + +0.3 3,136,987 + +1.9 3,137,004 + +0.4 3,137,004 + +0.3 3,137,012 - -1.4
Or see this region's nucleotide sequence