Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0008

Experiment: nitrate 250 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0007 and Psest_0008 are separated by 50 nucleotidesPsest_0008 and Psest_0009 overlap by 4 nucleotidesPsest_0009 and Psest_0010 overlap by 32 nucleotides Psest_0007: Psest_0007 - diguanylate cyclase (GGDEF) domain, at 8,642 to 10,012 _0007 Psest_0008: Psest_0008 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 10,063 to 10,812 _0008 Psest_0009: Psest_0009 - histidinol-phosphate phosphatase family domain/HAD-superfamily hydrolase, subfamily IIIA, at 10,809 to 11,387 _0009 Psest_0010: Psest_0010 - glycyl-tRNA synthetase, tetrameric type, beta subunit, at 11,356 to 13,410 _0010 Position (kb) 10 11Strain fitness (log2 ratio) -2 -1 0 1at 9.083 kb on + strand, within Psest_0007at 9.083 kb on + strand, within Psest_0007at 9.247 kb on + strand, within Psest_0007at 9.281 kb on + strand, within Psest_0007at 9.281 kb on + strand, within Psest_0007at 9.281 kb on + strand, within Psest_0007at 9.281 kb on + strand, within Psest_0007at 9.282 kb on - strand, within Psest_0007at 9.282 kb on - strand, within Psest_0007at 9.282 kb on - strand, within Psest_0007at 9.282 kb on - strand, within Psest_0007at 9.285 kb on + strand, within Psest_0007at 9.285 kb on + strand, within Psest_0007at 9.285 kb on + strand, within Psest_0007at 9.285 kb on + strand, within Psest_0007at 9.285 kb on + strand, within Psest_0007at 9.285 kb on + strand, within Psest_0007at 9.285 kb on + strand, within Psest_0007at 9.286 kb on - strand, within Psest_0007at 9.286 kb on - strand, within Psest_0007at 9.286 kb on - strand, within Psest_0007at 9.544 kb on - strand, within Psest_0007at 9.883 kb on - strandat 9.883 kb on - strandat 10.001 kb on + strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.002 kb on - strandat 10.013 kb on - strandat 10.828 kb on - strandat 10.828 kb on - strandat 10.828 kb on - strandat 11.358 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 250 mM
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9,083 + Psest_0007 0.32 -0.2
9,083 + Psest_0007 0.32 -2.4
9,247 + Psest_0007 0.44 -1.4
9,281 + Psest_0007 0.47 -1.1
9,281 + Psest_0007 0.47 -1.1
9,281 + Psest_0007 0.47 -2.1
9,281 + Psest_0007 0.47 +0.1
9,282 - Psest_0007 0.47 -0.5
9,282 - Psest_0007 0.47 +1.4
9,282 - Psest_0007 0.47 -0.4
9,282 - Psest_0007 0.47 -1.0
9,285 + Psest_0007 0.47 +1.3
9,285 + Psest_0007 0.47 +0.1
9,285 + Psest_0007 0.47 +0.2
9,285 + Psest_0007 0.47 -0.5
9,285 + Psest_0007 0.47 -1.7
9,285 + Psest_0007 0.47 -0.7
9,285 + Psest_0007 0.47 +0.2
9,286 - Psest_0007 0.47 +0.5
9,286 - Psest_0007 0.47 -0.0
9,286 - Psest_0007 0.47 +0.1
9,544 - Psest_0007 0.66 -0.3
9,883 - -0.2
9,883 - -0.2
10,001 + +0.8
10,002 - -0.8
10,002 - -0.5
10,002 - -2.0
10,002 - -0.1
10,002 - -1.2
10,013 - -1.5
10,828 - -1.3
10,828 - -0.1
10,828 - -1.8
11,358 - +0.0

Or see this region's nucleotide sequence