Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0642

Experiment: nitrate 250 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0641 and Psest_0642 are separated by 109 nucleotidesPsest_0642 and Psest_0643 overlap by 4 nucleotidesPsest_0643 and Psest_0644 are separated by 138 nucleotides Psest_0641: Psest_0641 - 1-deoxy-D-xylulose-5-phosphate synthase, at 695,426 to 697,324 _0641 Psest_0642: Psest_0642 - Geranylgeranyl pyrophosphate synthase, at 697,434 to 698,324 _0642 Psest_0643: Psest_0643 - exodeoxyribonuclease VII, small subunit, at 698,321 to 698,563 _0643 Psest_0644: Psest_0644 - siderophore ferric iron reductase, AHA_1954 family, at 698,702 to 699,463 _0644 Position (kb) 697 698 699Strain fitness (log2 ratio) -1 0 1 2at 698.607 kb on + strandat 698.608 kb on - strandat 698.655 kb on + strandat 698.656 kb on - strandat 698.656 kb on - strandat 698.668 kb on - strandat 698.668 kb on - strandat 699.200 kb on + strand, within Psest_0644at 699.200 kb on + strand, within Psest_0644at 699.220 kb on - strand, within Psest_0644at 699.220 kb on - strand, within Psest_0644at 699.220 kb on - strand, within Psest_0644at 699.220 kb on - strand, within Psest_0644at 699.264 kb on + strand, within Psest_0644at 699.264 kb on + strand, within Psest_0644

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 250 mM
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698,607 + +1.0
698,608 - -1.5
698,655 + +2.0
698,656 - +0.2
698,656 - +0.2
698,668 - +0.4
698,668 - +1.0
699,200 + Psest_0644 0.65 -1.7
699,200 + Psest_0644 0.65 +2.0
699,220 - Psest_0644 0.68 -0.4
699,220 - Psest_0644 0.68 -0.0
699,220 - Psest_0644 0.68 +1.6
699,220 - Psest_0644 0.68 -0.4
699,264 + Psest_0644 0.74 -1.2
699,264 + Psest_0644 0.74 -0.4

Or see this region's nucleotide sequence