Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0639 and Psest_0640 are separated by 116 nucleotides Psest_0640 and Psest_0641 are separated by 267 nucleotides Psest_0641 and Psest_0642 are separated by 109 nucleotides Psest_0642 and Psest_0643 overlap by 4 nucleotides
Psest_0639: Psest_0639 - Uncharacterised protein family (UPF0259)., at 693,885 to 694,565
_0639
Psest_0640: Psest_0640 - Uncharacterized conserved protein, at 694,682 to 695,158
_0640
Psest_0641: Psest_0641 - 1-deoxy-D-xylulose-5-phosphate synthase, at 695,426 to 697,324
_0641
Psest_0642: Psest_0642 - Geranylgeranyl pyrophosphate synthase, at 697,434 to 698,324
_0642
Psest_0643: Psest_0643 - exodeoxyribonuclease VII, small subunit, at 698,321 to 698,563
_0643
Position (kb) 695
696
697
698 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 694.525 kb on + strand at 694.525 kb on + strand at 694.721 kb on + strand at 694.721 kb on + strand at 694.721 kb on + strand at 694.721 kb on + strand at 694.721 kb on + strand at 694.722 kb on - strand at 694.722 kb on - strand at 694.724 kb on + strand at 694.724 kb on + strand at 694.724 kb on + strand at 694.724 kb on + strand at 694.725 kb on - strand at 694.725 kb on - strand at 694.725 kb on - strand at 694.760 kb on + strand, within Psest_0640 at 694.760 kb on + strand, within Psest_0640 at 694.760 kb on + strand, within Psest_0640 at 694.811 kb on + strand, within Psest_0640 at 694.811 kb on + strand, within Psest_0640 at 694.811 kb on + strand, within Psest_0640 at 694.812 kb on - strand, within Psest_0640 at 694.812 kb on - strand, within Psest_0640 at 694.817 kb on + strand, within Psest_0640 at 694.817 kb on + strand, within Psest_0640 at 694.901 kb on + strand, within Psest_0640 at 694.901 kb on + strand, within Psest_0640 at 694.901 kb on + strand, within Psest_0640 at 694.902 kb on - strand, within Psest_0640 at 694.902 kb on - strand, within Psest_0640 at 695.120 kb on + strand at 695.120 kb on + strand at 695.219 kb on + strand at 695.219 kb on + strand at 695.219 kb on + strand at 695.219 kb on + strand at 695.220 kb on - strand at 695.220 kb on - strand at 695.300 kb on + strand at 695.301 kb on - strand at 695.301 kb on - strand at 695.301 kb on - strand at 695.302 kb on + strand at 695.302 kb on + strand at 695.303 kb on - strand at 695.303 kb on - strand at 695.350 kb on + strand at 695.350 kb on + strand at 695.350 kb on + strand at 695.350 kb on + strand at 695.350 kb on + strand at 695.389 kb on - strand at 695.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 694,525 + -0.6 694,525 + +1.1 694,721 + -0.6 694,721 + -0.0 694,721 + +0.7 694,721 + +0.9 694,721 + +0.6 694,722 - +0.2 694,722 - -0.6 694,724 + +1.1 694,724 + -0.0 694,724 + +0.9 694,724 + -0.8 694,725 - -0.6 694,725 - +0.4 694,725 - +0.4 694,760 + Psest_0640 0.16 +0.8 694,760 + Psest_0640 0.16 -1.5 694,760 + Psest_0640 0.16 +1.6 694,811 + Psest_0640 0.27 +1.2 694,811 + Psest_0640 0.27 +1.2 694,811 + Psest_0640 0.27 +0.7 694,812 - Psest_0640 0.27 +1.0 694,812 - Psest_0640 0.27 +2.0 694,817 + Psest_0640 0.28 -0.0 694,817 + Psest_0640 0.28 -0.4 694,901 + Psest_0640 0.46 +1.2 694,901 + Psest_0640 0.46 +0.1 694,901 + Psest_0640 0.46 -0.0 694,902 - Psest_0640 0.46 +0.8 694,902 - Psest_0640 0.46 +1.0 695,120 + +3.0 695,120 + +0.4 695,219 + +3.0 695,219 + +0.6 695,219 + +2.0 695,219 + +1.0 695,220 - -1.0 695,220 - -1.3 695,300 + -0.0 695,301 - +0.4 695,301 - -0.6 695,301 - -1.8 695,302 + +0.5 695,302 + +0.4 695,303 - +2.0 695,303 - +1.0 695,350 + +1.2 695,350 + +0.4 695,350 + +1.4 695,350 + +2.4 695,350 + +2.0 695,389 - -0.2 695,389 - -0.8
Or see this region's nucleotide sequence