Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0399 and Psest_0400 are separated by 105 nucleotides Psest_0400 and Psest_0401 are separated by 158 nucleotides
Psest_0399: Psest_0399 - Acyl-CoA dehydrogenases, at 432,761 to 434,575
_0399
Psest_0400: Psest_0400 - Geranylgeranyl pyrophosphate synthase, at 434,681 to 435,571
_0400
Psest_0401: Psest_0401 - isopentenyl-diphosphate delta-isomerase, type 2, at 435,730 to 436,770
_0401
Position (kb) 434
435
436 Strain fitness (log2 ratio) -1
0
1
2
3 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.710 kb on + strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 433.711 kb on - strand, within Psest_0399 at 434.421 kb on + strand at 434.422 kb on - strand at 434.422 kb on - strand at 434.422 kb on - strand at 434.529 kb on + strand at 434.529 kb on + strand at 434.606 kb on + strand at 434.606 kb on + strand at 434.630 kb on + strand at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.086 kb on + strand, within Psest_0400 at 435.087 kb on - strand, within Psest_0400 at 435.377 kb on + strand, within Psest_0400 at 435.377 kb on + strand, within Psest_0400 at 435.386 kb on + strand, within Psest_0400 at 435.386 kb on + strand, within Psest_0400 at 435.454 kb on + strand, within Psest_0400 at 435.455 kb on - strand, within Psest_0400 at 435.625 kb on - strand at 435.640 kb on + strand at 435.641 kb on - strand at 435.675 kb on + strand at 436.223 kb on + strand, within Psest_0401
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 433,710 + Psest_0399 0.52 +1.0 433,710 + Psest_0399 0.52 -0.8 433,710 + Psest_0399 0.52 +1.7 433,710 + Psest_0399 0.52 -0.8 433,710 + Psest_0399 0.52 -0.7 433,711 - Psest_0399 0.52 -1.4 433,711 - Psest_0399 0.52 +1.1 433,711 - Psest_0399 0.52 +1.6 433,711 - Psest_0399 0.52 -0.6 433,711 - Psest_0399 0.52 -0.5 434,421 + -0.2 434,422 - -0.3 434,422 - -0.7 434,422 - -0.1 434,529 + -0.8 434,529 + -0.8 434,606 + +2.0 434,606 + +0.8 434,630 + +2.0 435,086 + Psest_0400 0.45 +0.7 435,086 + Psest_0400 0.45 +1.0 435,086 + Psest_0400 0.45 +0.8 435,086 + Psest_0400 0.45 -0.0 435,086 + Psest_0400 0.45 -1.5 435,087 - Psest_0400 0.46 +0.1 435,377 + Psest_0400 0.78 +0.7 435,377 + Psest_0400 0.78 +0.4 435,386 + Psest_0400 0.79 -0.0 435,386 + Psest_0400 0.79 +1.6 435,454 + Psest_0400 0.87 -0.3 435,455 - Psest_0400 0.87 +3.1 435,625 - +2.0 435,640 + -0.3 435,641 - -0.8 435,675 + +0.4 436,223 + Psest_0401 0.47 +2.0
Or see this region's nucleotide sequence