Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3545 and Psest_3546 are separated by 86 nucleotides Psest_3546 and Psest_3547 are separated by 52 nucleotides Psest_3547 and Psest_3548 are separated by 12 nucleotides Psest_3548 and Psest_3549 overlap by 4 nucleotides
Psest_3545: Psest_3545 - clan AA aspartic protease, TIGR02281 family, at 3,725,487 to 3,726,134
_3545
Psest_3546: Psest_3546 - Phosphatidylglycerophosphatase A and related proteins, at 3,726,221 to 3,726,718
_3546
Psest_3547: Psest_3547 - thiamine-monophosphate kinase, at 3,726,771 to 3,727,733
_3547
Psest_3548: Psest_3548 - transcription antitermination factor NusB, at 3,727,746 to 3,728,231
_3548
Psest_3549: Psest_3549 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,728,228 to 3,728,704
_3549
Position (kb) 3726
3727
3728 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 3725.878 kb on + strand, within Psest_3545 at 3725.879 kb on - strand, within Psest_3545 at 3725.908 kb on + strand, within Psest_3545 at 3725.908 kb on + strand, within Psest_3545 at 3725.908 kb on + strand, within Psest_3545 at 3725.909 kb on - strand, within Psest_3545 at 3725.909 kb on - strand, within Psest_3545 at 3726.129 kb on - strand at 3726.138 kb on + strand at 3726.138 kb on + strand at 3726.139 kb on - strand at 3726.139 kb on - strand at 3726.139 kb on - strand at 3726.139 kb on - strand at 3726.139 kb on - strand at 3726.139 kb on - strand at 3726.590 kb on + strand, within Psest_3546 at 3726.591 kb on - strand, within Psest_3546
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 3,725,878 + Psest_3545 0.60 +0.0 3,725,879 - Psest_3545 0.60 +3.8 3,725,908 + Psest_3545 0.65 +0.4 3,725,908 + Psest_3545 0.65 -0.7 3,725,908 + Psest_3545 0.65 -0.6 3,725,909 - Psest_3545 0.65 +0.0 3,725,909 - Psest_3545 0.65 +0.0 3,726,129 - +1.3 3,726,138 + -0.4 3,726,138 + +0.4 3,726,139 - -0.5 3,726,139 - +0.6 3,726,139 - -0.8 3,726,139 - +1.0 3,726,139 - -0.6 3,726,139 - -2.1 3,726,590 + Psest_3546 0.74 -1.2 3,726,591 - Psest_3546 0.74 +0.2
Or see this region's nucleotide sequence