Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3139 and Psest_3140 are separated by 48 nucleotides Psest_3140 and Psest_3141 are separated by 108 nucleotides Psest_3141 and Psest_3142 are separated by 58 nucleotides Psest_3142 and Psest_3143 are separated by 51 nucleotides
Psest_3139: Psest_3139 - Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily, at 3,314,196 to 3,314,717
_3139
Psest_3140: Psest_3140 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 3,314,766 to 3,315,491
_3140
Psest_3141: Psest_3141 - ADP-ribose pyrophosphatase, at 3,315,600 to 3,316,163
_3141
Psest_3142: Psest_3142 - Uncharacterized protein conserved in bacteria, at 3,316,222 to 3,316,725
_3142
Psest_3143: Psest_3143 - Uncharacterized protein conserved in bacteria, at 3,316,777 to 3,317,094
_3143
Position (kb)
3315
3316
3317 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3314.755 kb on - strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.837 kb on - strand at 3314.837 kb on - strand at 3314.837 kb on - strand at 3314.839 kb on - strand, within Psest_3140 at 3314.839 kb on - strand, within Psest_3140 at 3314.883 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.935 kb on + strand, within Psest_3140 at 3315.037 kb on + strand, within Psest_3140 at 3315.037 kb on + strand, within Psest_3140 at 3315.038 kb on - strand, within Psest_3140 at 3315.058 kb on + strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.216 kb on + strand, within Psest_3140 at 3315.217 kb on - strand, within Psest_3140 at 3315.350 kb on - strand, within Psest_3140 at 3315.369 kb on + strand, within Psest_3140 at 3315.369 kb on + strand, within Psest_3140 at 3315.370 kb on - strand, within Psest_3140 at 3315.370 kb on - strand, within Psest_3140 at 3315.452 kb on - strand at 3315.538 kb on - strand at 3315.607 kb on - strand at 3315.897 kb on + strand, within Psest_3141 at 3315.897 kb on + strand, within Psest_3141 at 3315.897 kb on + strand, within Psest_3141 at 3315.897 kb on + strand, within Psest_3141 at 3315.898 kb on - strand, within Psest_3141 at 3315.898 kb on - strand, within Psest_3141 at 3315.898 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.257 kb on + strand at 3316.257 kb on + strand at 3316.258 kb on - strand at 3316.287 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.482 kb on + strand, within Psest_3142 at 3316.482 kb on + strand, within Psest_3142 at 3316.483 kb on - strand, within Psest_3142 at 3316.483 kb on - strand, within Psest_3142 at 3316.483 kb on - strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.679 kb on + strand at 3316.679 kb on + strand at 3316.680 kb on - strand at 3316.680 kb on - strand at 3316.811 kb on + strand, within Psest_3143 at 3316.812 kb on - strand, within Psest_3143 at 3316.812 kb on - strand, within Psest_3143 at 3316.812 kb on - strand, within Psest_3143 at 3316.858 kb on - strand, within Psest_3143 at 3316.906 kb on + strand, within Psest_3143 at 3316.907 kb on - strand, within Psest_3143 at 3316.936 kb on - strand, within Psest_3143 at 3316.978 kb on + strand, within Psest_3143 at 3317.011 kb on + strand, within Psest_3143 at 3317.159 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 3,314,755 - -0.5 3,314,836 + +0.3 3,314,836 + +0.0 3,314,836 + -3.5 3,314,836 + -0.7 3,314,837 - -1.1 3,314,837 - +1.4 3,314,837 - -2.7 3,314,839 - Psest_3140 0.10 -1.9 3,314,839 - Psest_3140 0.10 -0.7 3,314,883 + Psest_3140 0.16 +0.9 3,314,917 + Psest_3140 0.21 -1.0 3,314,917 + Psest_3140 0.21 -1.2 3,314,917 + Psest_3140 0.21 +2.1 3,314,917 + Psest_3140 0.21 -0.1 3,314,917 + Psest_3140 0.21 +0.4 3,314,918 - Psest_3140 0.21 -1.7 3,314,918 - Psest_3140 0.21 -2.5 3,314,918 - Psest_3140 0.21 +0.7 3,314,918 - Psest_3140 0.21 -2.0 3,314,918 - Psest_3140 0.21 +0.4 3,314,918 - Psest_3140 0.21 -1.0 3,314,935 + Psest_3140 0.23 -2.5 3,315,037 + Psest_3140 0.37 -1.0 3,315,037 + Psest_3140 0.37 -1.1 3,315,038 - Psest_3140 0.37 +0.4 3,315,058 + Psest_3140 0.40 -0.6 3,315,059 - Psest_3140 0.40 -0.4 3,315,059 - Psest_3140 0.40 +0.1 3,315,059 - Psest_3140 0.40 -1.0 3,315,059 - Psest_3140 0.40 -0.7 3,315,059 - Psest_3140 0.40 -0.1 3,315,216 + Psest_3140 0.62 +0.7 3,315,217 - Psest_3140 0.62 +0.4 3,315,350 - Psest_3140 0.80 +0.0 3,315,369 + Psest_3140 0.83 +0.7 3,315,369 + Psest_3140 0.83 -0.1 3,315,370 - Psest_3140 0.83 +0.4 3,315,370 - Psest_3140 0.83 +0.1 3,315,452 - +1.3 3,315,538 - +1.1 3,315,607 - +1.0 3,315,897 + Psest_3141 0.53 -0.6 3,315,897 + Psest_3141 0.53 -1.1 3,315,897 + Psest_3141 0.53 -0.7 3,315,897 + Psest_3141 0.53 +0.8 3,315,898 - Psest_3141 0.53 +0.5 3,315,898 - Psest_3141 0.53 +1.5 3,315,898 - Psest_3141 0.53 +1.1 3,315,900 - Psest_3141 0.53 +1.2 3,315,900 - Psest_3141 0.53 +1.9 3,315,900 - Psest_3141 0.53 +0.9 3,315,900 - Psest_3141 0.53 +1.7 3,315,933 + Psest_3141 0.59 -1.0 3,315,933 + Psest_3141 0.59 +0.5 3,315,933 + Psest_3141 0.59 +0.1 3,315,933 + Psest_3141 0.59 -1.1 3,315,933 + Psest_3141 0.59 -1.4 3,315,933 + Psest_3141 0.59 -0.3 3,315,933 + Psest_3141 0.59 +2.3 3,315,933 + Psest_3141 0.59 -2.2 3,315,933 + Psest_3141 0.59 -0.5 3,315,933 + Psest_3141 0.59 +2.5 3,315,933 + Psest_3141 0.59 +0.5 3,315,933 + Psest_3141 0.59 -0.7 3,315,933 + Psest_3141 0.59 +0.6 3,315,933 + Psest_3141 0.59 +0.1 3,315,933 + Psest_3141 0.59 +0.5 3,315,933 + Psest_3141 0.59 -2.7 3,315,933 + Psest_3141 0.59 -2.2 3,315,933 + Psest_3141 0.59 +0.0 3,315,933 + Psest_3141 0.59 -1.7 3,315,934 - Psest_3141 0.59 +0.5 3,315,934 - Psest_3141 0.59 -0.0 3,315,934 - Psest_3141 0.59 +0.3 3,315,934 - Psest_3141 0.59 +1.3 3,315,934 - Psest_3141 0.59 +1.2 3,315,934 - Psest_3141 0.59 +1.2 3,315,934 - Psest_3141 0.59 +1.5 3,315,934 - Psest_3141 0.59 +0.4 3,315,934 - Psest_3141 0.59 +0.5 3,315,934 - Psest_3141 0.59 -0.1 3,315,934 - Psest_3141 0.59 +0.4 3,315,934 - Psest_3141 0.59 +1.4 3,315,934 - Psest_3141 0.59 -1.4 3,315,934 - Psest_3141 0.59 +0.7 3,315,934 - Psest_3141 0.59 +0.6 3,315,934 - Psest_3141 0.59 -0.1 3,315,934 - Psest_3141 0.59 +2.4 3,315,934 - Psest_3141 0.59 +0.7 3,316,064 - Psest_3141 0.82 -0.7 3,316,064 - Psest_3141 0.82 -0.5 3,316,064 - Psest_3141 0.82 -1.7 3,316,064 - Psest_3141 0.82 -1.4 3,316,064 - Psest_3141 0.82 +2.0 3,316,064 - Psest_3141 0.82 +0.9 3,316,064 - Psest_3141 0.82 +0.9 3,316,257 + +0.9 3,316,257 + +0.3 3,316,258 - -2.0 3,316,287 + Psest_3142 0.13 -0.5 3,316,453 + Psest_3142 0.46 -0.8 3,316,453 + Psest_3142 0.46 +1.9 3,316,453 + Psest_3142 0.46 +0.7 3,316,453 + Psest_3142 0.46 -0.3 3,316,453 + Psest_3142 0.46 +0.9 3,316,453 + Psest_3142 0.46 +0.7 3,316,453 + Psest_3142 0.46 +0.0 3,316,454 - Psest_3142 0.46 +1.2 3,316,454 - Psest_3142 0.46 -0.9 3,316,454 - Psest_3142 0.46 -0.1 3,316,454 - Psest_3142 0.46 +0.2 3,316,482 + Psest_3142 0.52 +0.8 3,316,482 + Psest_3142 0.52 +0.4 3,316,483 - Psest_3142 0.52 -0.6 3,316,483 - Psest_3142 0.52 -0.3 3,316,483 - Psest_3142 0.52 +1.1 3,316,486 + Psest_3142 0.52 +0.4 3,316,486 + Psest_3142 0.52 +0.6 3,316,486 + Psest_3142 0.52 -1.2 3,316,486 + Psest_3142 0.52 -0.9 3,316,486 + Psest_3142 0.52 -2.1 3,316,486 + Psest_3142 0.52 +0.3 3,316,486 + Psest_3142 0.52 +0.9 3,316,486 + Psest_3142 0.52 +0.2 3,316,486 + Psest_3142 0.52 +0.8 3,316,486 + Psest_3142 0.52 -0.1 3,316,486 + Psest_3142 0.52 -0.0 3,316,486 + Psest_3142 0.52 -0.1 3,316,486 + Psest_3142 0.52 -0.1 3,316,487 - Psest_3142 0.53 +0.0 3,316,487 - Psest_3142 0.53 -0.8 3,316,487 - Psest_3142 0.53 -1.4 3,316,487 - Psest_3142 0.53 +0.2 3,316,487 - Psest_3142 0.53 +1.0 3,316,487 - Psest_3142 0.53 -0.1 3,316,487 - Psest_3142 0.53 +0.9 3,316,487 - Psest_3142 0.53 -1.1 3,316,679 + +0.9 3,316,679 + +0.4 3,316,680 - +0.5 3,316,680 - +0.8 3,316,811 + Psest_3143 0.11 -1.0 3,316,812 - Psest_3143 0.11 +0.0 3,316,812 - Psest_3143 0.11 -1.3 3,316,812 - Psest_3143 0.11 -0.9 3,316,858 - Psest_3143 0.25 +0.5 3,316,906 + Psest_3143 0.41 -0.8 3,316,907 - Psest_3143 0.41 +2.1 3,316,936 - Psest_3143 0.50 +0.4 3,316,978 + Psest_3143 0.63 -1.9 3,317,011 + Psest_3143 0.74 +0.2 3,317,159 + -0.7
Or see this region's nucleotide sequence