Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0264 and Psest_0265 are separated by 68 nucleotides Psest_0265 and Psest_0266 are separated by 88 nucleotides Psest_0266 and Psest_0267 are separated by 74 nucleotides Psest_0267 and Psest_0268 are separated by 65 nucleotides
Psest_0264: Psest_0264 - monofunctional biosynthetic peptidoglycan transglycosylase, at 281,399 to 282,127
_0264
Psest_0265: Psest_0265 - Uncharacterized small membrane protein, at 282,196 to 282,570
_0265
Psest_0266: Psest_0266 - thiamine biosynthesis protein ThiS, at 282,659 to 282,859
_0266
Psest_0267: Psest_0267 - Uncharacterized enzyme of thiazole biosynthesis, at 282,934 to 283,728
_0267
Psest_0268: Psest_0268 - tRNA (guanine-N(7)-)-methyltransferase, at 283,794 to 284,510
_0268
Position (kb)
282
283
284 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 281.974 kb on + strand, within Psest_0264 at 282.056 kb on + strand at 282.056 kb on + strand at 282.057 kb on - strand at 282.057 kb on - strand at 282.160 kb on + strand at 282.160 kb on + strand at 282.160 kb on + strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.162 kb on + strand at 282.163 kb on - strand at 282.208 kb on + strand at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.299 kb on - strand, within Psest_0265 at 282.329 kb on - strand, within Psest_0265 at 282.454 kb on + strand, within Psest_0265 at 282.454 kb on + strand, within Psest_0265 at 282.455 kb on - strand, within Psest_0265 at 282.622 kb on - strand at 282.686 kb on + strand, within Psest_0266 at 282.686 kb on + strand, within Psest_0266 at 282.687 kb on - strand, within Psest_0266 at 282.726 kb on + strand, within Psest_0266 at 282.727 kb on - strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.837 kb on + strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.838 kb on - strand, within Psest_0266 at 282.889 kb on + strand at 282.890 kb on - strand at 282.892 kb on - strand at 282.909 kb on + strand at 282.909 kb on + strand at 282.910 kb on - strand at 282.982 kb on + strand at 282.982 kb on + strand at 283.015 kb on + strand, within Psest_0267 at 283.015 kb on + strand, within Psest_0267 at 283.015 kb on + strand, within Psest_0267 at 283.015 kb on + strand, within Psest_0267 at 283.150 kb on + strand, within Psest_0267 at 283.151 kb on - strand, within Psest_0267 at 283.280 kb on + strand, within Psest_0267 at 283.280 kb on + strand, within Psest_0267 at 283.351 kb on + strand, within Psest_0267 at 283.351 kb on + strand, within Psest_0267 at 283.351 kb on + strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.352 kb on - strand, within Psest_0267 at 283.459 kb on + strand, within Psest_0267 at 283.459 kb on + strand, within Psest_0267 at 283.460 kb on - strand, within Psest_0267 at 283.460 kb on - strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.630 kb on + strand, within Psest_0267 at 283.631 kb on - strand, within Psest_0267 at 283.631 kb on - strand, within Psest_0267 at 283.631 kb on - strand, within Psest_0267 at 283.658 kb on - strand at 283.687 kb on + strand at 283.788 kb on + strand at 283.788 kb on + strand at 283.789 kb on - strand at 283.789 kb on - strand at 284.079 kb on - strand, within Psest_0268 at 284.142 kb on + strand, within Psest_0268 at 284.142 kb on + strand, within Psest_0268 at 284.142 kb on + strand, within Psest_0268 at 284.142 kb on + strand, within Psest_0268 at 284.142 kb on + strand, within Psest_0268 at 284.142 kb on + strand, within Psest_0268 at 284.143 kb on - strand, within Psest_0268 at 284.143 kb on - strand, within Psest_0268 at 284.143 kb on - strand, within Psest_0268 at 284.143 kb on - strand, within Psest_0268 at 284.319 kb on - strand, within Psest_0268 at 284.349 kb on + strand, within Psest_0268 at 284.349 kb on + strand, within Psest_0268 at 284.350 kb on - strand, within Psest_0268 at 284.350 kb on - strand, within Psest_0268 at 284.350 kb on - strand, within Psest_0268 at 284.350 kb on - strand, within Psest_0268 at 284.479 kb on + strand at 284.537 kb on - strand at 284.537 kb on - strand at 284.537 kb on - strand at 284.538 kb on + strand at 284.608 kb on + strand at 284.668 kb on + strand at 284.668 kb on + strand at 284.668 kb on + strand at 284.668 kb on + strand at 284.669 kb on - strand at 284.669 kb on - strand at 284.669 kb on - strand at 284.669 kb on - strand at 284.669 kb on - strand at 284.669 kb on - strand at 284.670 kb on + strand at 284.670 kb on + strand at 284.670 kb on + strand at 284.671 kb on - strand at 284.671 kb on - strand at 284.671 kb on - strand at 284.671 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 281,974 + Psest_0264 0.79 -0.8 282,056 + -1.0 282,056 + -1.7 282,057 - -2.3 282,057 - -0.7 282,160 + +1.3 282,160 + -0.1 282,160 + +0.1 282,161 - +0.2 282,161 - -0.1 282,161 - +0.0 282,161 - +1.4 282,161 - +0.5 282,162 + +1.1 282,163 - +1.6 282,208 + +0.9 282,299 - Psest_0265 0.27 -0.6 282,299 - Psest_0265 0.27 +0.7 282,299 - Psest_0265 0.27 +0.3 282,299 - Psest_0265 0.27 +0.0 282,299 - Psest_0265 0.27 -1.2 282,299 - Psest_0265 0.27 +0.1 282,329 - Psest_0265 0.35 -2.0 282,454 + Psest_0265 0.69 +1.1 282,454 + Psest_0265 0.69 +0.5 282,455 - Psest_0265 0.69 +0.4 282,622 - +0.7 282,686 + Psest_0266 0.13 -1.0 282,686 + Psest_0266 0.13 -0.7 282,687 - Psest_0266 0.14 -2.0 282,726 + Psest_0266 0.33 -1.0 282,727 - Psest_0266 0.34 -1.3 282,837 + Psest_0266 0.89 -0.5 282,837 + Psest_0266 0.89 -1.8 282,837 + Psest_0266 0.89 +0.7 282,837 + Psest_0266 0.89 -0.8 282,838 - Psest_0266 0.89 -0.3 282,838 - Psest_0266 0.89 -0.3 282,838 - Psest_0266 0.89 -2.6 282,838 - Psest_0266 0.89 -0.1 282,838 - Psest_0266 0.89 -2.1 282,838 - Psest_0266 0.89 +0.5 282,838 - Psest_0266 0.89 -2.4 282,889 + -0.8 282,890 - +0.5 282,892 - -1.0 282,909 + -0.4 282,909 + -1.8 282,910 - -2.5 282,982 + -0.5 282,982 + -0.7 283,015 + Psest_0267 0.10 -0.9 283,015 + Psest_0267 0.10 +0.1 283,015 + Psest_0267 0.10 -2.1 283,015 + Psest_0267 0.10 -1.3 283,150 + Psest_0267 0.27 -2.0 283,151 - Psest_0267 0.27 +0.2 283,280 + Psest_0267 0.44 -1.5 283,280 + Psest_0267 0.44 -0.2 283,351 + Psest_0267 0.52 -0.3 283,351 + Psest_0267 0.52 -0.2 283,351 + Psest_0267 0.52 +1.1 283,352 - Psest_0267 0.53 +0.2 283,352 - Psest_0267 0.53 -1.3 283,352 - Psest_0267 0.53 +1.6 283,352 - Psest_0267 0.53 -0.1 283,352 - Psest_0267 0.53 +1.4 283,352 - Psest_0267 0.53 -0.3 283,352 - Psest_0267 0.53 +0.5 283,352 - Psest_0267 0.53 -2.1 283,352 - Psest_0267 0.53 -1.1 283,352 - Psest_0267 0.53 -0.2 283,459 + Psest_0267 0.66 -0.9 283,459 + Psest_0267 0.66 -0.4 283,460 - Psest_0267 0.66 -1.3 283,460 - Psest_0267 0.66 +2.9 283,630 + Psest_0267 0.88 +0.8 283,630 + Psest_0267 0.88 -1.1 283,630 + Psest_0267 0.88 -1.2 283,630 + Psest_0267 0.88 -1.6 283,630 + Psest_0267 0.88 +0.3 283,630 + Psest_0267 0.88 -0.6 283,631 - Psest_0267 0.88 -0.6 283,631 - Psest_0267 0.88 -1.0 283,631 - Psest_0267 0.88 -0.7 283,658 - -0.5 283,687 + -0.7 283,788 + -2.1 283,788 + +1.8 283,789 - +0.3 283,789 - +1.3 284,079 - Psest_0268 0.40 +1.7 284,142 + Psest_0268 0.49 -1.4 284,142 + Psest_0268 0.49 +1.3 284,142 + Psest_0268 0.49 +0.2 284,142 + Psest_0268 0.49 +1.5 284,142 + Psest_0268 0.49 +1.0 284,142 + Psest_0268 0.49 +0.7 284,143 - Psest_0268 0.49 +0.2 284,143 - Psest_0268 0.49 -0.4 284,143 - Psest_0268 0.49 -0.7 284,143 - Psest_0268 0.49 +0.2 284,319 - Psest_0268 0.73 +0.6 284,349 + Psest_0268 0.77 -1.6 284,349 + Psest_0268 0.77 +0.7 284,350 - Psest_0268 0.78 +2.1 284,350 - Psest_0268 0.78 +1.5 284,350 - Psest_0268 0.78 -0.2 284,350 - Psest_0268 0.78 +0.2 284,479 + +0.7 284,537 - -0.6 284,537 - -0.1 284,537 - -0.3 284,538 + -1.6 284,608 + -0.4 284,668 + -2.8 284,668 + -3.4 284,668 + -2.7 284,668 + -0.9 284,669 - -0.1 284,669 - +0.4 284,669 - -0.4 284,669 - +1.3 284,669 - +0.4 284,669 - +1.3 284,670 + -0.7 284,670 + -0.7 284,670 + -1.2 284,671 - +0.3 284,671 - +1.3 284,671 - +0.3 284,671 - -0.7
Or see this region's nucleotide sequence