Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2000 and Psest_2001 are separated by 80 nucleotides Psest_2001 and Psest_2002 overlap by 1 nucleotides Psest_2002 and Psest_2003 are separated by 143 nucleotides
Psest_2000: Psest_2000 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,117,371 to 2,118,111
_2000
Psest_2001: Psest_2001 - 2-phosphoglycolate phosphatase, prokaryotic, at 2,118,192 to 2,118,863
_2001
Psest_2002: Psest_2002 - ubiquinone biosynthesis O-methyltransferase, at 2,118,863 to 2,119,561
_2002
Psest_2003: Psest_2003 - Cytosine deaminase and related metal-dependent hydrolases, at 2,119,705 to 2,121,048
_2003
Position (kb)
2118
2119
2120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2117.873 kb on + strand, within Psest_2000 at 2117.952 kb on - strand, within Psest_2000 at 2117.989 kb on + strand, within Psest_2000 at 2117.990 kb on - strand, within Psest_2000 at 2117.992 kb on + strand, within Psest_2000 at 2117.992 kb on + strand, within Psest_2000 at 2118.102 kb on - strand at 2118.124 kb on - strand at 2118.124 kb on - strand at 2118.135 kb on + strand at 2118.136 kb on - strand at 2118.136 kb on - strand at 2118.138 kb on + strand at 2118.188 kb on + strand at 2118.295 kb on + strand, within Psest_2001 at 2118.295 kb on + strand, within Psest_2001 at 2118.295 kb on + strand, within Psest_2001 at 2118.347 kb on + strand, within Psest_2001 at 2118.437 kb on + strand, within Psest_2001 at 2118.590 kb on + strand, within Psest_2001 at 2118.874 kb on + strand at 2118.874 kb on + strand at 2118.874 kb on + strand at 2118.874 kb on + strand at 2119.645 kb on + strand at 2119.645 kb on + strand at 2119.645 kb on + strand at 2119.645 kb on + strand at 2119.646 kb on - strand at 2119.646 kb on - strand at 2119.646 kb on - strand at 2119.646 kb on - strand at 2119.647 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.663 kb on + strand at 2119.664 kb on - strand at 2119.664 kb on - strand at 2119.664 kb on - strand at 2119.664 kb on - strand at 2119.664 kb on - strand at 2119.664 kb on - strand at 2119.664 kb on - strand at 2119.664 kb on - strand at 2119.671 kb on - strand at 2119.859 kb on + strand, within Psest_2003 at 2119.859 kb on + strand, within Psest_2003 at 2119.859 kb on + strand, within Psest_2003 at 2119.859 kb on + strand, within Psest_2003 at 2119.859 kb on + strand, within Psest_2003 at 2119.859 kb on + strand, within Psest_2003 at 2119.860 kb on - strand, within Psest_2003 at 2119.860 kb on - strand, within Psest_2003 at 2119.883 kb on + strand, within Psest_2003 at 2119.883 kb on + strand, within Psest_2003 at 2119.883 kb on + strand, within Psest_2003 at 2119.883 kb on + strand, within Psest_2003 at 2119.883 kb on + strand, within Psest_2003 at 2119.883 kb on + strand, within Psest_2003 at 2119.884 kb on - strand, within Psest_2003 at 2119.884 kb on - strand, within Psest_2003 at 2119.942 kb on + strand, within Psest_2003 at 2120.140 kb on - strand, within Psest_2003 at 2120.140 kb on - strand, within Psest_2003 at 2120.140 kb on - strand, within Psest_2003 at 2120.186 kb on - strand, within Psest_2003 at 2120.186 kb on - strand, within Psest_2003 at 2120.226 kb on + strand, within Psest_2003 at 2120.226 kb on + strand, within Psest_2003 at 2120.226 kb on + strand, within Psest_2003 at 2120.227 kb on - strand, within Psest_2003 at 2120.261 kb on - strand, within Psest_2003 at 2120.271 kb on + strand, within Psest_2003 at 2120.271 kb on + strand, within Psest_2003 at 2120.271 kb on + strand, within Psest_2003 at 2120.271 kb on + strand, within Psest_2003 at 2120.272 kb on - strand, within Psest_2003 at 2120.272 kb on - strand, within Psest_2003 at 2120.272 kb on - strand, within Psest_2003 at 2120.456 kb on + strand, within Psest_2003 at 2120.457 kb on - strand, within Psest_2003 at 2120.533 kb on + strand, within Psest_2003
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 2,117,873 + Psest_2000 0.68 +0.4 2,117,952 - Psest_2000 0.78 +1.8 2,117,989 + Psest_2000 0.83 +1.8 2,117,990 - Psest_2000 0.84 +2.2 2,117,992 + Psest_2000 0.84 +1.0 2,117,992 + Psest_2000 0.84 +1.1 2,118,102 - +1.6 2,118,124 - +0.8 2,118,124 - +2.0 2,118,135 + +1.1 2,118,136 - -0.2 2,118,136 - +0.7 2,118,138 + +1.5 2,118,188 + +1.9 2,118,295 + Psest_2001 0.15 +1.2 2,118,295 + Psest_2001 0.15 -0.5 2,118,295 + Psest_2001 0.15 -0.1 2,118,347 + Psest_2001 0.23 -2.1 2,118,437 + Psest_2001 0.36 -1.5 2,118,590 + Psest_2001 0.59 +1.0 2,118,874 + -2.0 2,118,874 + -2.2 2,118,874 + +3.3 2,118,874 + -0.3 2,119,645 + -1.6 2,119,645 + -1.2 2,119,645 + -0.2 2,119,645 + -0.2 2,119,646 - +3.0 2,119,646 - -0.4 2,119,646 - +1.5 2,119,646 - -0.3 2,119,647 + -0.9 2,119,663 + -1.5 2,119,663 + +0.1 2,119,663 + -0.1 2,119,663 + -1.1 2,119,663 + -1.3 2,119,663 + -0.4 2,119,663 + -2.6 2,119,663 + -2.0 2,119,663 + -0.6 2,119,663 + -0.7 2,119,663 + -1.4 2,119,664 - -0.9 2,119,664 - +0.9 2,119,664 - +0.8 2,119,664 - +0.0 2,119,664 - +0.1 2,119,664 - -2.0 2,119,664 - +0.9 2,119,664 - -0.5 2,119,671 - -0.0 2,119,859 + Psest_2003 0.11 -2.4 2,119,859 + Psest_2003 0.11 -2.0 2,119,859 + Psest_2003 0.11 -2.2 2,119,859 + Psest_2003 0.11 -0.9 2,119,859 + Psest_2003 0.11 -0.4 2,119,859 + Psest_2003 0.11 -3.6 2,119,860 - Psest_2003 0.12 -0.3 2,119,860 - Psest_2003 0.12 -0.5 2,119,883 + Psest_2003 0.13 -0.3 2,119,883 + Psest_2003 0.13 -2.4 2,119,883 + Psest_2003 0.13 +0.3 2,119,883 + Psest_2003 0.13 -0.8 2,119,883 + Psest_2003 0.13 -0.9 2,119,883 + Psest_2003 0.13 -0.9 2,119,884 - Psest_2003 0.13 -1.2 2,119,884 - Psest_2003 0.13 -1.2 2,119,942 + Psest_2003 0.18 -1.7 2,120,140 - Psest_2003 0.32 +1.2 2,120,140 - Psest_2003 0.32 +0.3 2,120,140 - Psest_2003 0.32 +0.7 2,120,186 - Psest_2003 0.36 -1.4 2,120,186 - Psest_2003 0.36 +0.1 2,120,226 + Psest_2003 0.39 -0.9 2,120,226 + Psest_2003 0.39 -2.8 2,120,226 + Psest_2003 0.39 -1.3 2,120,227 - Psest_2003 0.39 -2.4 2,120,261 - Psest_2003 0.41 -2.1 2,120,271 + Psest_2003 0.42 -2.4 2,120,271 + Psest_2003 0.42 -1.5 2,120,271 + Psest_2003 0.42 +0.1 2,120,271 + Psest_2003 0.42 -0.9 2,120,272 - Psest_2003 0.42 -0.6 2,120,272 - Psest_2003 0.42 -0.6 2,120,272 - Psest_2003 0.42 +0.5 2,120,456 + Psest_2003 0.56 -2.3 2,120,457 - Psest_2003 0.56 -1.3 2,120,533 + Psest_2003 0.62 -2.7
Or see this region's nucleotide sequence