Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0188 and Psest_0189 are separated by 112 nucleotides Psest_0189 and Psest_0190 are separated by 2 nucleotides Psest_0190 and Psest_0191 are separated by 21 nucleotides
Psest_0188: Psest_0188 - diguanylate cyclase (GGDEF) domain, at 183,244 to 185,142
_0188
Psest_0189: Psest_0189 - Negative regulator of beta-lactamase expression, at 185,255 to 186,043
_0189
Psest_0190: Psest_0190 - Uncharacterized conserved protein, at 186,046 to 186,450
_0190
Psest_0191: Psest_0191 - PAS domain S-box, at 186,472 to 188,259
_0191
Position (kb) 185
186
187 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 184.306 kb on + strand, within Psest_0188 at 184.307 kb on - strand, within Psest_0188 at 184.524 kb on + strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 185.178 kb on - strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.864 kb on + strand, within Psest_0189 at 185.961 kb on - strand, within Psest_0189 at 186.051 kb on - strand at 186.293 kb on + strand, within Psest_0190 at 186.293 kb on + strand, within Psest_0190 at 186.354 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.565 kb on + strand at 186.645 kb on - strand at 186.735 kb on + strand, within Psest_0191
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 184,306 + Psest_0188 0.56 +0.8 184,307 - Psest_0188 0.56 +0.2 184,524 + Psest_0188 0.67 +1.1 184,529 - Psest_0188 0.68 +1.8 184,529 - Psest_0188 0.68 +1.8 185,178 - +0.6 185,332 + +4.3 185,332 + +1.4 185,332 + +0.7 185,332 + +2.3 185,332 + +1.8 185,332 + +1.3 185,333 - +3.2 185,333 - +2.8 185,333 - +2.8 185,333 - +0.8 185,333 - +3.4 185,333 - +0.6 185,378 + Psest_0189 0.16 +2.6 185,378 + Psest_0189 0.16 +3.6 185,378 + Psest_0189 0.16 +1.7 185,378 + Psest_0189 0.16 +1.4 185,378 + Psest_0189 0.16 -1.6 185,379 - Psest_0189 0.16 +1.8 185,379 - Psest_0189 0.16 +0.0 185,379 - Psest_0189 0.16 +0.4 185,379 - Psest_0189 0.16 +0.8 185,379 - Psest_0189 0.16 -0.2 185,379 - Psest_0189 0.16 +3.1 185,379 - Psest_0189 0.16 +1.7 185,379 - Psest_0189 0.16 +1.8 185,379 - Psest_0189 0.16 +3.1 185,442 - Psest_0189 0.24 +1.6 185,442 - Psest_0189 0.24 +1.4 185,864 + Psest_0189 0.77 +1.8 185,961 - Psest_0189 0.89 +1.4 186,051 - -0.8 186,293 + Psest_0190 0.61 +0.7 186,293 + Psest_0190 0.61 +1.5 186,354 + Psest_0190 0.76 +1.1 186,356 + Psest_0190 0.77 -0.1 186,356 + Psest_0190 0.77 +1.5 186,356 + Psest_0190 0.77 +0.8 186,491 + +0.7 186,491 + +0.7 186,491 + -0.3 186,491 + +0.8 186,492 - +1.0 186,492 - +0.3 186,492 - +0.5 186,492 - +0.2 186,492 - +1.2 186,492 - +0.8 186,492 - +0.2 186,492 - +0.2 186,492 - +1.2 186,565 + +1.9 186,645 - +0.2 186,735 + Psest_0191 0.15 +3.2
Or see this region's nucleotide sequence