Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0145 and Psest_0146 overlap by 4 nucleotides Psest_0146 and Psest_0147 are separated by 191 nucleotides Psest_0147 and Psest_0148 are separated by 88 nucleotides
Psest_0145: Psest_0145 - A/G-specific adenine glycosylase, at 143,312 to 144,379
_0145
Psest_0146: Psest_0146 - Uncharacterized protein involved in outer membrane biogenesis, at 144,376 to 146,601
_0146
Psest_0147: Psest_0147 - Acetyltransferase, GNAT family., at 146,793 to 147,191
_0147
Psest_0148: Psest_0148 - nitrate transport ATP-binding subunits C and D, at 147,280 to 148,062
_0148
Position (kb)
144
145
146
147 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 143.407 kb on + strand at 143.408 kb on - strand at 143.409 kb on + strand at 143.409 kb on + strand at 143.409 kb on + strand at 143.410 kb on - strand at 143.410 kb on - strand at 143.410 kb on - strand at 143.410 kb on - strand at 143.841 kb on + strand, within Psest_0145 at 143.842 kb on - strand, within Psest_0145 at 143.842 kb on - strand, within Psest_0145 at 143.937 kb on + strand, within Psest_0145 at 143.938 kb on - strand, within Psest_0145 at 143.938 kb on - strand, within Psest_0145 at 144.133 kb on - strand, within Psest_0145 at 144.136 kb on - strand, within Psest_0145 at 144.575 kb on + strand at 144.647 kb on + strand, within Psest_0146 at 144.647 kb on + strand, within Psest_0146 at 145.076 kb on - strand, within Psest_0146 at 145.076 kb on - strand, within Psest_0146 at 145.076 kb on - strand, within Psest_0146 at 145.112 kb on + strand, within Psest_0146 at 145.112 kb on + strand, within Psest_0146 at 145.113 kb on - strand, within Psest_0146 at 145.255 kb on + strand, within Psest_0146 at 146.030 kb on + strand, within Psest_0146 at 146.031 kb on - strand, within Psest_0146 at 146.031 kb on - strand, within Psest_0146 at 146.057 kb on + strand, within Psest_0146 at 146.058 kb on - strand, within Psest_0146 at 146.058 kb on - strand, within Psest_0146 at 146.058 kb on - strand, within Psest_0146 at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.498 kb on + strand at 146.499 kb on - strand at 146.499 kb on - strand at 146.499 kb on - strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.615 kb on + strand at 146.616 kb on - strand at 146.616 kb on - strand at 146.616 kb on - strand at 146.642 kb on + strand at 146.643 kb on - strand at 146.643 kb on - strand at 146.644 kb on + strand at 146.644 kb on + strand at 146.644 kb on + strand at 146.795 kb on - strand at 147.087 kb on - strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.091 kb on + strand, within Psest_0147 at 147.092 kb on - strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.124 kb on + strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.125 kb on - strand, within Psest_0147 at 147.145 kb on - strand, within Psest_0147 at 147.181 kb on + strand at 147.181 kb on + strand at 147.181 kb on + strand at 147.182 kb on - strand at 147.231 kb on + strand at 147.338 kb on + strand at 147.338 kb on + strand at 147.339 kb on - strand at 147.339 kb on - strand at 147.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 143,407 + -2.0 143,408 - -1.1 143,409 + -1.1 143,409 + +0.1 143,409 + +0.9 143,410 - -1.0 143,410 - -1.6 143,410 - +0.6 143,410 - -1.4 143,841 + Psest_0145 0.50 -0.6 143,842 - Psest_0145 0.50 -0.0 143,842 - Psest_0145 0.50 -0.5 143,937 + Psest_0145 0.59 -1.6 143,938 - Psest_0145 0.59 -0.6 143,938 - Psest_0145 0.59 +0.0 144,133 - Psest_0145 0.77 +0.8 144,136 - Psest_0145 0.77 -1.5 144,575 + -1.9 144,647 + Psest_0146 0.12 -2.5 144,647 + Psest_0146 0.12 -0.2 145,076 - Psest_0146 0.31 -1.9 145,076 - Psest_0146 0.31 -2.6 145,076 - Psest_0146 0.31 -1.9 145,112 + Psest_0146 0.33 -0.5 145,112 + Psest_0146 0.33 -1.6 145,113 - Psest_0146 0.33 -1.4 145,255 + Psest_0146 0.39 -1.3 146,030 + Psest_0146 0.74 -1.3 146,031 - Psest_0146 0.74 -3.8 146,031 - Psest_0146 0.74 -2.4 146,057 + Psest_0146 0.76 -2.3 146,058 - Psest_0146 0.76 -2.5 146,058 - Psest_0146 0.76 -1.2 146,058 - Psest_0146 0.76 +0.8 146,498 + +0.0 146,498 + -1.8 146,498 + -1.4 146,498 + -1.4 146,498 + -1.2 146,498 + -2.3 146,498 + -2.5 146,499 - -1.4 146,499 - -3.7 146,499 - -2.5 146,615 + -2.6 146,615 + -0.8 146,615 + -1.7 146,615 + -0.5 146,615 + -0.2 146,615 + -0.5 146,615 + -1.2 146,615 + -2.1 146,615 + +1.2 146,616 - -0.9 146,616 - -0.7 146,616 - -0.2 146,642 + +0.2 146,643 - -0.4 146,643 - -0.5 146,644 + +0.5 146,644 + -0.7 146,644 + -0.5 146,795 - -1.8 147,087 - Psest_0147 0.74 -0.5 147,091 + Psest_0147 0.75 -0.2 147,091 + Psest_0147 0.75 -0.0 147,092 - Psest_0147 0.75 -2.7 147,124 + Psest_0147 0.83 +0.6 147,124 + Psest_0147 0.83 -1.4 147,124 + Psest_0147 0.83 -0.1 147,125 - Psest_0147 0.83 -1.7 147,125 - Psest_0147 0.83 +0.3 147,125 - Psest_0147 0.83 -0.4 147,125 - Psest_0147 0.83 -0.2 147,125 - Psest_0147 0.83 -0.8 147,125 - Psest_0147 0.83 -1.2 147,145 - Psest_0147 0.88 -3.0 147,181 + -0.8 147,181 + -1.5 147,181 + -0.8 147,182 - -1.0 147,231 + +1.9 147,338 + -0.4 147,338 + +0.3 147,339 - +0.9 147,339 - -1.7 147,339 - +0.5
Or see this region's nucleotide sequence