Experiment: nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1321 and Psest_1322 are separated by 278 nucleotides Psest_1322 and Psest_1323 overlap by 4 nucleotides Psest_1323 and Psest_1324 are separated by 121 nucleotides
Psest_1321: Psest_1321 - undecaprenyl-diphosphatase UppP, at 1,421,885 to 1,422,697
_1321
Psest_1322: Psest_1322 - 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases, at 1,422,976 to 1,423,863
_1322
Psest_1323: Psest_1323 - Hydroxypyruvate isomerase, at 1,423,860 to 1,424,642
_1323
Psest_1324: Psest_1324 - Arabinose efflux permease, at 1,424,764 to 1,426,110
_1324
Position (kb)
1422
1423
1424 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1422.557 kb on + strand, within Psest_1321 at 1423.127 kb on + strand, within Psest_1322 at 1423.359 kb on + strand, within Psest_1322 at 1423.467 kb on + strand, within Psest_1322 at 1423.843 kb on + strand at 1423.843 kb on + strand at 1423.843 kb on + strand at 1423.843 kb on + strand at 1423.844 kb on - strand at 1423.844 kb on - strand at 1423.844 kb on - strand at 1423.844 kb on - strand at 1424.116 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.133 kb on + strand, within Psest_1323 at 1424.134 kb on - strand, within Psest_1323 at 1424.134 kb on - strand, within Psest_1323 at 1424.395 kb on - strand, within Psest_1323 at 1424.400 kb on - strand, within Psest_1323 at 1424.578 kb on - strand at 1424.699 kb on + strand at 1424.699 kb on + strand at 1424.699 kb on + strand at 1424.699 kb on + strand at 1424.700 kb on - strand at 1424.700 kb on - strand at 1424.739 kb on + strand at 1424.739 kb on + strand at 1424.739 kb on + strand at 1424.739 kb on + strand at 1424.740 kb on - strand at 1424.740 kb on - strand at 1424.740 kb on - strand at 1424.740 kb on - strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.741 kb on + strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand at 1424.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 250 mM remove 1,422,557 + Psest_1321 0.83 -1.2 1,423,127 + Psest_1322 0.17 +1.1 1,423,359 + Psest_1322 0.43 -0.7 1,423,467 + Psest_1322 0.55 -0.3 1,423,843 + -0.7 1,423,843 + -0.4 1,423,843 + +0.1 1,423,843 + +0.5 1,423,844 - -1.3 1,423,844 - +0.6 1,423,844 - -2.2 1,423,844 - -0.5 1,424,116 + Psest_1323 0.33 -0.9 1,424,133 + Psest_1323 0.35 -2.7 1,424,133 + Psest_1323 0.35 -0.7 1,424,133 + Psest_1323 0.35 +0.7 1,424,133 + Psest_1323 0.35 -0.6 1,424,133 + Psest_1323 0.35 -1.1 1,424,133 + Psest_1323 0.35 -0.2 1,424,134 - Psest_1323 0.35 -1.1 1,424,134 - Psest_1323 0.35 -1.4 1,424,395 - Psest_1323 0.68 -1.7 1,424,400 - Psest_1323 0.69 -0.2 1,424,578 - -0.1 1,424,699 + -2.6 1,424,699 + -0.7 1,424,699 + -1.9 1,424,699 + -0.9 1,424,700 - +0.7 1,424,700 - +0.0 1,424,739 + -0.2 1,424,739 + -1.0 1,424,739 + -0.7 1,424,739 + -0.0 1,424,740 - +2.1 1,424,740 - -0.8 1,424,740 - +0.1 1,424,740 - -2.3 1,424,741 + -0.2 1,424,741 + -2.4 1,424,741 + -1.2 1,424,741 + +0.7 1,424,741 + -1.7 1,424,741 + -0.7 1,424,742 - -2.7 1,424,742 - -2.0 1,424,742 - -2.3 1,424,742 - +0.1 1,424,742 - -1.0 1,424,742 - -0.2
Or see this region's nucleotide sequence