Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3474 and Dshi_3475 overlap by 4 nucleotides Dshi_3475 and Dshi_3476 are separated by 9 nucleotides Dshi_3476 and Dshi_3477 are separated by 2 nucleotides Dshi_3477 and Dshi_3478 overlap by 4 nucleotides
Dshi_3474: Dshi_3474 - fumarate lyase (RefSeq), at 3,657,613 to 3,658,659
_3474
Dshi_3475: Dshi_3475 - protein of unknown function DUF849 (RefSeq), at 3,658,656 to 3,659,495
_3475
Dshi_3476: Dshi_3476 - protocatechuate 3,4-dioxygenase, alpha subunit (RefSeq), at 3,659,505 to 3,660,119
_3476
Dshi_3477: Dshi_3477 - protocatechuate 3,4-dioxygenase, beta subunit (RefSeq), at 3,660,122 to 3,660,859
_3477
Dshi_3478: Dshi_3478 - 4-carboxymuconolactone decarboxylase (RefSeq), at 3,660,856 to 3,661,242
_3478
Position (kb)
3659
3660
3661 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3658.508 kb on + strand, within Dshi_3474 at 3658.511 kb on - strand, within Dshi_3474 at 3658.597 kb on + strand at 3658.597 kb on + strand at 3658.597 kb on + strand at 3658.605 kb on - strand at 3658.605 kb on - strand at 3658.610 kb on + strand at 3658.662 kb on - strand at 3658.662 kb on - strand at 3658.669 kb on + strand at 3658.684 kb on - strand at 3658.780 kb on - strand, within Dshi_3475 at 3658.885 kb on + strand, within Dshi_3475 at 3658.903 kb on + strand, within Dshi_3475 at 3658.911 kb on - strand, within Dshi_3475 at 3658.958 kb on - strand, within Dshi_3475 at 3658.996 kb on - strand, within Dshi_3475 at 3659.002 kb on + strand, within Dshi_3475 at 3659.054 kb on + strand, within Dshi_3475 at 3659.071 kb on + strand, within Dshi_3475 at 3659.082 kb on - strand, within Dshi_3475 at 3659.213 kb on + strand, within Dshi_3475 at 3659.247 kb on + strand, within Dshi_3475 at 3659.282 kb on - strand, within Dshi_3475 at 3659.353 kb on - strand, within Dshi_3475 at 3659.353 kb on - strand, within Dshi_3475 at 3659.400 kb on - strand, within Dshi_3475 at 3659.428 kb on + strand at 3659.431 kb on - strand at 3659.476 kb on + strand at 3659.512 kb on - strand at 3659.580 kb on - strand, within Dshi_3476 at 3659.580 kb on - strand, within Dshi_3476 at 3659.599 kb on - strand, within Dshi_3476 at 3659.641 kb on + strand, within Dshi_3476 at 3659.684 kb on + strand, within Dshi_3476 at 3659.697 kb on + strand, within Dshi_3476 at 3659.697 kb on + strand, within Dshi_3476 at 3659.721 kb on + strand, within Dshi_3476 at 3659.721 kb on + strand, within Dshi_3476 at 3659.729 kb on - strand, within Dshi_3476 at 3659.729 kb on - strand, within Dshi_3476 at 3659.729 kb on - strand, within Dshi_3476 at 3659.729 kb on - strand, within Dshi_3476 at 3659.729 kb on - strand, within Dshi_3476 at 3659.866 kb on - strand, within Dshi_3476 at 3659.876 kb on - strand, within Dshi_3476 at 3659.964 kb on + strand, within Dshi_3476 at 3659.978 kb on - strand, within Dshi_3476 at 3659.986 kb on + strand, within Dshi_3476 at 3659.988 kb on - strand, within Dshi_3476 at 3660.029 kb on + strand, within Dshi_3476 at 3660.031 kb on + strand, within Dshi_3476 at 3660.038 kb on + strand, within Dshi_3476 at 3660.038 kb on + strand, within Dshi_3476 at 3660.046 kb on - strand, within Dshi_3476 at 3660.046 kb on - strand, within Dshi_3476 at 3660.047 kb on - strand, within Dshi_3476 at 3660.069 kb on - strand at 3660.084 kb on - strand at 3660.105 kb on - strand at 3660.108 kb on + strand at 3660.153 kb on + strand at 3660.160 kb on - strand at 3660.172 kb on + strand at 3660.199 kb on + strand, within Dshi_3477 at 3660.207 kb on - strand, within Dshi_3477 at 3660.215 kb on - strand, within Dshi_3477 at 3660.220 kb on - strand, within Dshi_3477 at 3660.288 kb on + strand, within Dshi_3477 at 3660.376 kb on - strand, within Dshi_3477 at 3660.376 kb on - strand, within Dshi_3477 at 3660.417 kb on - strand, within Dshi_3477 at 3660.497 kb on - strand, within Dshi_3477 at 3660.540 kb on + strand, within Dshi_3477 at 3660.605 kb on + strand, within Dshi_3477 at 3660.613 kb on - strand, within Dshi_3477 at 3660.618 kb on - strand, within Dshi_3477 at 3660.649 kb on - strand, within Dshi_3477 at 3660.682 kb on + strand, within Dshi_3477 at 3660.735 kb on + strand, within Dshi_3477 at 3660.777 kb on + strand, within Dshi_3477 at 3660.841 kb on + strand at 3660.863 kb on - strand at 3660.863 kb on - strand at 3660.863 kb on - strand at 3660.927 kb on - strand, within Dshi_3478 at 3660.952 kb on - strand, within Dshi_3478 at 3660.954 kb on + strand, within Dshi_3478 at 3660.962 kb on - strand, within Dshi_3478 at 3660.967 kb on + strand, within Dshi_3478 at 3660.978 kb on + strand, within Dshi_3478 at 3660.983 kb on + strand, within Dshi_3478 at 3661.044 kb on + strand, within Dshi_3478 at 3661.090 kb on + strand, within Dshi_3478
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 3,658,508 + Dshi_3474 0.85 +0.5 3,658,511 - Dshi_3474 0.86 -0.6 3,658,597 + +0.6 3,658,597 + -1.2 3,658,597 + -2.7 3,658,605 - +1.7 3,658,605 - -0.9 3,658,610 + +1.2 3,658,662 - -1.9 3,658,662 - +0.0 3,658,669 + +1.3 3,658,684 - +0.6 3,658,780 - Dshi_3475 0.15 +0.5 3,658,885 + Dshi_3475 0.27 +0.6 3,658,903 + Dshi_3475 0.29 +0.2 3,658,911 - Dshi_3475 0.30 +0.5 3,658,958 - Dshi_3475 0.36 -0.2 3,658,996 - Dshi_3475 0.40 -1.5 3,659,002 + Dshi_3475 0.41 +1.3 3,659,054 + Dshi_3475 0.47 -2.4 3,659,071 + Dshi_3475 0.49 -0.6 3,659,082 - Dshi_3475 0.51 +0.6 3,659,213 + Dshi_3475 0.66 -1.9 3,659,247 + Dshi_3475 0.70 -1.2 3,659,282 - Dshi_3475 0.75 -0.7 3,659,353 - Dshi_3475 0.83 -1.8 3,659,353 - Dshi_3475 0.83 -0.2 3,659,400 - Dshi_3475 0.89 -1.3 3,659,428 + -1.1 3,659,431 - -0.4 3,659,476 + -0.9 3,659,512 - -1.8 3,659,580 - Dshi_3476 0.12 -0.3 3,659,580 - Dshi_3476 0.12 -0.7 3,659,599 - Dshi_3476 0.15 -0.3 3,659,641 + Dshi_3476 0.22 -0.7 3,659,684 + Dshi_3476 0.29 -0.3 3,659,697 + Dshi_3476 0.31 +1.3 3,659,697 + Dshi_3476 0.31 -0.6 3,659,721 + Dshi_3476 0.35 +3.0 3,659,721 + Dshi_3476 0.35 +0.7 3,659,729 - Dshi_3476 0.36 +0.1 3,659,729 - Dshi_3476 0.36 +0.6 3,659,729 - Dshi_3476 0.36 -0.5 3,659,729 - Dshi_3476 0.36 +0.5 3,659,729 - Dshi_3476 0.36 +0.1 3,659,866 - Dshi_3476 0.59 +0.4 3,659,876 - Dshi_3476 0.60 +0.8 3,659,964 + Dshi_3476 0.75 +0.4 3,659,978 - Dshi_3476 0.77 -0.0 3,659,986 + Dshi_3476 0.78 -2.0 3,659,988 - Dshi_3476 0.79 -1.0 3,660,029 + Dshi_3476 0.85 -0.0 3,660,031 + Dshi_3476 0.86 -1.3 3,660,038 + Dshi_3476 0.87 +0.4 3,660,038 + Dshi_3476 0.87 +0.4 3,660,046 - Dshi_3476 0.88 -0.0 3,660,046 - Dshi_3476 0.88 +0.1 3,660,047 - Dshi_3476 0.88 -3.0 3,660,069 - +1.3 3,660,084 - -0.4 3,660,105 - -0.8 3,660,108 + +0.1 3,660,153 + -1.0 3,660,160 - -0.1 3,660,172 + -2.4 3,660,199 + Dshi_3477 0.10 -0.0 3,660,207 - Dshi_3477 0.12 -0.6 3,660,215 - Dshi_3477 0.13 +0.7 3,660,220 - Dshi_3477 0.13 -0.5 3,660,288 + Dshi_3477 0.22 +0.6 3,660,376 - Dshi_3477 0.34 -1.4 3,660,376 - Dshi_3477 0.34 -0.5 3,660,417 - Dshi_3477 0.40 +0.1 3,660,497 - Dshi_3477 0.51 -1.4 3,660,540 + Dshi_3477 0.57 -0.6 3,660,605 + Dshi_3477 0.65 -1.1 3,660,613 - Dshi_3477 0.67 +0.0 3,660,618 - Dshi_3477 0.67 -0.0 3,660,649 - Dshi_3477 0.71 -1.6 3,660,682 + Dshi_3477 0.76 -0.4 3,660,735 + Dshi_3477 0.83 +0.1 3,660,777 + Dshi_3477 0.89 +0.1 3,660,841 + -0.9 3,660,863 - +0.4 3,660,863 - -0.2 3,660,863 - -0.3 3,660,927 - Dshi_3478 0.18 -0.3 3,660,952 - Dshi_3478 0.25 -3.2 3,660,954 + Dshi_3478 0.25 +2.3 3,660,962 - Dshi_3478 0.27 +0.7 3,660,967 + Dshi_3478 0.29 +1.4 3,660,978 + Dshi_3478 0.32 +0.3 3,660,983 + Dshi_3478 0.33 -0.2 3,661,044 + Dshi_3478 0.49 +1.4 3,661,090 + Dshi_3478 0.60 -1.3
Or see this region's nucleotide sequence