Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_R0022 and Sama_R0023 are separated by 70 nucleotides Sama_R0023 and Sama_R0024 are separated by 119 nucleotides Sama_R0024 and Sama_R0025 are separated by 276 nucleotides Sama_R0025 and Sama_R0026 are separated by 66 nucleotides Sama_R0026 and Sama_R0027 are separated by 66 nucleotides Sama_R0027 and Sama_R0028 are separated by 45 nucleotides Sama_R0028 and Sama_R0029 are separated by 42 nucleotides Sama_R0029 and Sama_0363 are separated by 129 nucleotides
Sama_R0022: Sama_R0022 - 16S ribosomal RNA (RefSeq), at 436,076 to 437,612
_R0022
Sama_R0023: Sama_R0023 - tRNA-Ile (RefSeq), at 437,683 to 437,759
_R0023
Sama_R0024: Sama_R0024 - tRNA-Ala (RefSeq), at 437,879 to 437,954
_R0024
Sama_R0025: Sama_R0025 - 23S ribosomal RNA (RefSeq), at 438,231 to 441,144
_R0025
Sama_R0026: Sama_R0026 - tRNA-Val (RefSeq), at 441,211 to 441,286
_R0026
Sama_R0027: Sama_R0027 - 5S ribosomal RNA (RefSeq), at 441,353 to 441,468
_R0027
Sama_R0028: Sama_R0028 - tRNA-Thr (RefSeq), at 441,514 to 441,589
_R0028
Sama_R0029: Sama_R0029 - 5S ribosomal RNA (RefSeq), at 441,632 to 441,746
_R0029
Sama_0363: Sama_0363 - mutator MutT protein (RefSeq), at 441,876 to 442,271
_0363
Position (kb) 438
439
440
441
442 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.287 kb on + strand at 441.453 kb on + strand, within Sama_R0027 at 441.453 kb on + strand, within Sama_R0027 at 441.472 kb on + strand at 441.549 kb on + strand, within Sama_R0028 at 441.549 kb on + strand, within Sama_R0028 at 441.591 kb on + strand at 441.591 kb on + strand at 441.608 kb on + strand at 441.608 kb on + strand at 441.679 kb on + strand, within Sama_R0029 at 441.679 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.704 kb on + strand, within Sama_R0029 at 441.730 kb on + strand, within Sama_R0029 at 441.730 kb on + strand, within Sama_R0029 at 441.788 kb on + strand at 441.819 kb on + strand at 441.831 kb on - strand at 441.846 kb on + strand at 441.846 kb on + strand at 441.881 kb on + strand at 441.881 kb on + strand at 441.882 kb on - strand at 441.882 kb on - strand at 441.882 kb on - strand at 441.899 kb on + strand at 441.899 kb on + strand at 441.935 kb on - strand, within Sama_0363 at 441.935 kb on - strand, within Sama_0363 at 442.022 kb on + strand, within Sama_0363 at 442.023 kb on - strand, within Sama_0363 at 442.023 kb on - strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.032 kb on + strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.033 kb on - strand, within Sama_0363 at 442.049 kb on - strand, within Sama_0363 at 442.067 kb on + strand, within Sama_0363 at 442.067 kb on + strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.068 kb on - strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.069 kb on + strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.070 kb on - strand, within Sama_0363 at 442.111 kb on - strand, within Sama_0363
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 441,287 + -0.9 441,287 + +0.3 441,287 + -1.7 441,287 + -0.1 441,287 + -2.2 441,287 + -0.7 441,287 + +0.1 441,287 + -0.4 441,453 + Sama_R0027 0.86 -0.3 441,453 + Sama_R0027 0.86 +0.5 441,472 + +3.5 441,549 + Sama_R0028 0.46 -0.6 441,549 + Sama_R0028 0.46 +4.2 441,591 + -1.0 441,591 + -0.3 441,608 + -0.5 441,608 + -0.6 441,679 + Sama_R0029 0.41 -0.8 441,679 + Sama_R0029 0.41 -1.3 441,704 + Sama_R0029 0.63 -0.5 441,704 + Sama_R0029 0.63 +1.0 441,704 + Sama_R0029 0.63 -1.1 441,730 + Sama_R0029 0.85 -1.4 441,730 + Sama_R0029 0.85 -0.1 441,788 + +1.6 441,819 + +1.2 441,831 - +1.5 441,846 + -1.6 441,846 + -1.5 441,881 + -1.6 441,881 + -2.2 441,882 - -2.0 441,882 - -0.4 441,882 - +0.1 441,899 + -2.0 441,899 + +1.5 441,935 - Sama_0363 0.15 -1.8 441,935 - Sama_0363 0.15 -0.4 442,022 + Sama_0363 0.37 -0.5 442,023 - Sama_0363 0.37 -1.8 442,023 - Sama_0363 0.37 -1.2 442,032 + Sama_0363 0.39 -0.7 442,032 + Sama_0363 0.39 -1.0 442,032 + Sama_0363 0.39 -2.4 442,033 - Sama_0363 0.40 +3.7 442,033 - Sama_0363 0.40 -0.7 442,033 - Sama_0363 0.40 +1.4 442,049 - Sama_0363 0.44 -0.3 442,067 + Sama_0363 0.48 -2.0 442,067 + Sama_0363 0.48 -1.8 442,068 - Sama_0363 0.48 -0.9 442,068 - Sama_0363 0.48 -1.4 442,068 - Sama_0363 0.48 -1.2 442,068 - Sama_0363 0.48 +5.1 442,068 - Sama_0363 0.48 -1.8 442,069 + Sama_0363 0.49 +1.3 442,069 + Sama_0363 0.49 +2.9 442,069 + Sama_0363 0.49 -0.9 442,069 + Sama_0363 0.49 +2.0 442,069 + Sama_0363 0.49 +1.5 442,069 + Sama_0363 0.49 -1.8 442,069 + Sama_0363 0.49 -2.8 442,070 - Sama_0363 0.49 -2.3 442,070 - Sama_0363 0.49 +3.4 442,070 - Sama_0363 0.49 +0.4 442,070 - Sama_0363 0.49 -1.5 442,070 - Sama_0363 0.49 +0.1 442,070 - Sama_0363 0.49 +6.2 442,070 - Sama_0363 0.49 +0.2 442,070 - Sama_0363 0.49 +1.8 442,070 - Sama_0363 0.49 -0.9 442,070 - Sama_0363 0.49 +3.0 442,070 - Sama_0363 0.49 -1.7 442,070 - Sama_0363 0.49 -1.6 442,070 - Sama_0363 0.49 +1.0 442,111 - Sama_0363 0.59 -0.9
Or see this region's nucleotide sequence