Strain Fitness in Dinoroseobacter shibae DFL-12 around Dshi_3380

Experiment: pyruvate (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	pyruvate (C)
3,552,413	+		Dshi_3379	0.30	+0.3
3,552,418	+		Dshi_3379	0.31	+0.6
3,552,431	-		Dshi_3379	0.32	+0.7
3,552,498	-		Dshi_3379	0.37	-0.6
3,552,500	-		Dshi_3379	0.37	-1.3
3,552,536	+		Dshi_3379	0.40	+0.1
3,552,561	-		Dshi_3379	0.42	-0.7
3,552,616	-		Dshi_3379	0.46	-2.4
3,552,633	-		Dshi_3379	0.47	-0.6
3,552,640	+		Dshi_3379	0.48	-0.6
3,552,649	-		Dshi_3379	0.49	-0.5
3,552,649	-		Dshi_3379	0.49	-0.4
3,552,649	-		Dshi_3379	0.49	+0.6
3,552,654	-		Dshi_3379	0.49	-1.5
3,552,654	-		Dshi_3379	0.49	-0.2
3,552,657	+		Dshi_3379	0.49	+0.1
3,552,657	-		Dshi_3379	0.49	-1.3
3,552,669	-		Dshi_3379	0.50	-0.3
3,552,670	+		Dshi_3379	0.50	+0.7
3,552,672	+		Dshi_3379	0.50	-0.7
3,552,672	+		Dshi_3379	0.50	-0.3
3,552,678	-		Dshi_3379	0.51	-2.1
3,552,680	-		Dshi_3379	0.51	+1.5
3,552,685	+		Dshi_3379	0.51	-0.3
3,552,685	+		Dshi_3379	0.51	-0.4
3,552,693	-		Dshi_3379	0.52	+1.3
3,552,704	-		Dshi_3379	0.53	-0.6
3,552,714	-		Dshi_3379	0.53	-1.8
3,552,792	+		Dshi_3379	0.59	-0.5
3,552,794	-		Dshi_3379	0.60	-0.0
3,552,800	-		Dshi_3379	0.60	-0.7
3,552,831	+		Dshi_3379	0.62	-0.4
3,552,837	+		Dshi_3379	0.63	+1.1
3,552,839	-		Dshi_3379	0.63	-1.5
3,552,860	-		Dshi_3379	0.65	-1.5
3,552,893	+		Dshi_3379	0.67	-1.0
3,552,919	+		Dshi_3379	0.69	-1.0
3,552,991	+		Dshi_3379	0.75	-0.7
3,552,991	+		Dshi_3379	0.75	+0.9
3,552,998	-		Dshi_3379	0.75	+0.6
3,552,999	-		Dshi_3379	0.75	-0.6
3,553,012	+		Dshi_3379	0.76	-0.9
3,553,017	+		Dshi_3379	0.77	+0.9
3,553,032	+		Dshi_3379	0.78	+0.3
3,553,037	+		Dshi_3379	0.78	+0.6
3,553,040	-		Dshi_3379	0.78	+0.3
3,553,041	-		Dshi_3379	0.79	+1.6
3,553,048	+		Dshi_3379	0.79	-0.1
3,553,048	+		Dshi_3379	0.79	+0.2
3,553,049	+		Dshi_3379	0.79	-2.6
3,553,055	+		Dshi_3379	0.80	-0.1
3,553,056	-		Dshi_3379	0.80	-0.8
3,553,079	+		Dshi_3379	0.81	+0.3
3,553,082	-		Dshi_3379	0.82	-1.9
3,553,095	-		Dshi_3379	0.83	-1.8
3,553,107	+		Dshi_3379	0.84	-0.5
3,553,117	+		Dshi_3379	0.84	-0.4
3,553,127	+		Dshi_3379	0.85	-0.4
3,553,127	+		Dshi_3379	0.85	-0.5
3,553,127	+		Dshi_3379	0.85	+0.6
3,553,130	-		Dshi_3379	0.85	-0.3
3,553,135	-		Dshi_3379	0.86	-0.4
3,553,142	+		Dshi_3379	0.86	+1.5
3,553,142	+		Dshi_3379	0.86	-0.2
3,553,143	-		Dshi_3379	0.86	+0.8
3,553,145	-		Dshi_3379	0.87	-1.3
3,553,150	-		Dshi_3379	0.87	+0.5
3,553,151	+		Dshi_3379	0.87	+0.2
3,553,151	-		Dshi_3379	0.87	+0.2
3,553,156	+		Dshi_3379	0.87	-0.1
3,553,157	+		Dshi_3379	0.87	-0.3
3,553,163	+		Dshi_3379	0.88	-1.4
3,553,208	+				+0.2
3,553,209	+				-1.2
3,553,209	+				-0.6
3,553,216	+				-0.7
3,553,216	+				-0.8
3,553,217	-				-0.2
3,553,217	-				-0.5
3,553,217	-				+0.9
3,553,221	+				+0.3
3,553,221	+				-0.4
3,553,221	+				-0.9
3,553,221	+				+0.9
3,553,222	+				-1.4
3,553,223	+				+0.3
3,553,229	-				+0.3
3,553,229	-				+0.1
3,553,229	-				+0.3
3,553,229	-				+0.3
3,553,231	-				+0.6
3,553,231	-				+0.0
3,553,231	-				+1.3
3,553,239	-				+0.6
3,553,250	+				-0.3
3,553,260	+				+0.2
3,553,260	+				+1.0
3,553,260	+				+0.2
3,553,268	-				-0.3
3,553,282	+				-0.3
3,553,290	-				-1.5
3,553,290	-				-0.3
3,553,290	-				-1.4
3,553,304	-				+0.9
3,553,310	-				+0.1
3,553,327	-				-1.1
3,553,328	-				-2.5
3,553,354	-				+0.0
3,553,354	-				-0.4
3,553,358	+				-1.3
3,553,358	+				-0.7
3,553,359	+				-0.3
3,553,359	-				+0.5
3,553,364	+				+0.8
3,553,366	-				+0.6
3,553,366	-				-0.1
3,553,366	-				+0.6
3,553,366	-				+0.1
3,553,366	-				-0.7
3,553,367	-				-0.5
3,553,372	+				-0.7
3,553,373	+				-0.3
3,553,373	+				-0.9
3,553,373	+				+0.6
3,553,373	+				+0.1
3,553,373	+				+0.0
3,553,381	-				+0.8
3,553,381	-				-0.1
3,553,381	-				-0.6
3,553,381	-				+0.3
3,553,381	-				-0.5
3,553,381	-				+0.6
3,553,381	-				-0.5
3,553,381	-				-1.2
3,553,382	-				+0.9
3,553,382	-				+0.4
3,553,383	+				+0.7
3,553,383	+				+0.7
3,553,383	+				-0.9
3,553,383	+				-1.0
3,553,383	+				+0.2
3,553,383	+				-1.5
3,553,383	+				-0.1
3,553,383	+				+0.6
3,553,383	+				-1.1
3,553,383	+				+0.6
3,553,383	+				-0.4
3,553,383	+				+0.9
3,553,383	+				+0.4
3,553,383	+				-0.8
3,553,383	+				-1.8
3,553,383	+				-0.7
3,553,383	+				-2.2
3,553,383	+				+0.3
3,553,383	+				+0.3
3,553,391	-				-0.5
3,553,391	-				-0.4
3,553,391	-				-1.8
3,553,391	-				-2.2
3,553,391	-				-0.4
3,553,391	-				-1.7
3,553,391	-				-0.1
3,553,391	-				-1.8
3,553,391	-				-1.4
3,553,391	-				-1.8
3,553,391	-				-0.9
3,553,391	-				-0.7
3,553,391	-				-0.8
3,553,391	-				-0.9
3,553,391	-				-0.8
3,553,480	+				+0.1
3,553,488	-		Dshi_3380	0.10	-0.5
3,553,488	-		Dshi_3380	0.10	-0.6
3,553,490	+		Dshi_3380	0.11	-0.1
3,553,526	-		Dshi_3380	0.15	+0.8
3,553,529	+		Dshi_3380	0.15	+0.6
3,553,530	+		Dshi_3380	0.15	+0.1
3,553,530	+		Dshi_3380	0.15	-0.7
3,553,530	+		Dshi_3380	0.15	-0.1
3,553,536	-		Dshi_3380	0.16	-1.3
3,553,537	-		Dshi_3380	0.16	-0.9
3,553,537	-		Dshi_3380	0.16	-0.2
3,553,537	-		Dshi_3380	0.16	-0.4
3,553,537	-		Dshi_3380	0.16	-1.8
3,553,538	+		Dshi_3380	0.16	+0.5
3,553,538	+		Dshi_3380	0.16	+0.3
3,553,538	+		Dshi_3380	0.16	+0.6
3,553,539	+		Dshi_3380	0.16	+0.2
3,553,539	+		Dshi_3380	0.16	-0.9
3,553,539	+		Dshi_3380	0.16	-0.2
3,553,539	+		Dshi_3380	0.16	+0.2
3,553,539	+		Dshi_3380	0.16	+0.3
3,553,539	+		Dshi_3380	0.16	-0.0
3,553,539	+		Dshi_3380	0.16	-1.9
3,553,539	+		Dshi_3380	0.16	+0.3
3,553,539	+		Dshi_3380	0.16	-0.2
3,553,540	-		Dshi_3380	0.17	-0.4
3,553,541	+		Dshi_3380	0.17	-1.5
3,553,541	+		Dshi_3380	0.17	-0.6
3,553,541	+		Dshi_3380	0.17	-0.7
3,553,543	+		Dshi_3380	0.17	-1.0
3,553,544	-		Dshi_3380	0.17	+0.2
3,553,544	-		Dshi_3380	0.17	+1.5
3,553,546	-		Dshi_3380	0.17	+0.5
3,553,546	-		Dshi_3380	0.17	+0.6
3,553,546	-		Dshi_3380	0.17	-0.5
3,553,547	-		Dshi_3380	0.17	-0.2
3,553,547	-		Dshi_3380	0.17	+0.3
3,553,547	-		Dshi_3380	0.17	-2.4
3,553,547	-		Dshi_3380	0.17	+1.8
3,553,547	-		Dshi_3380	0.17	-1.7
3,553,547	-		Dshi_3380	0.17	+1.3
3,553,547	-		Dshi_3380	0.17	-1.2
3,553,549	-		Dshi_3380	0.18	-0.5
3,553,549	-		Dshi_3380	0.18	+0.3
3,553,549	-		Dshi_3380	0.18	-1.8
3,553,549	-		Dshi_3380	0.18	-0.8
3,553,551	+		Dshi_3380	0.18	+1.3
3,553,556	-		Dshi_3380	0.19	+0.1
3,553,559	-		Dshi_3380	0.19	-0.8
3,553,563	+		Dshi_3380	0.19	-1.2
3,553,563	+		Dshi_3380	0.19	-1.1
3,553,563	+		Dshi_3380	0.19	-0.1
3,553,563	+		Dshi_3380	0.19	+0.3
3,553,563	+		Dshi_3380	0.19	-0.7
3,553,566	-		Dshi_3380	0.20	+0.4
3,553,566	-		Dshi_3380	0.20	+1.7
3,553,571	-		Dshi_3380	0.20	+0.1
3,553,571	-		Dshi_3380	0.20	-0.1
3,553,571	-		Dshi_3380	0.20	+0.7
3,553,571	-		Dshi_3380	0.20	-1.2
3,553,571	-		Dshi_3380	0.20	-0.7
3,553,571	-		Dshi_3380	0.20	-1.2
3,553,571	-		Dshi_3380	0.20	-0.1
3,553,571	-		Dshi_3380	0.20	+0.3
3,553,573	+		Dshi_3380	0.21	-1.1
3,553,573	+		Dshi_3380	0.21	+1.4
3,553,578	-		Dshi_3380	0.21	-0.6
3,553,588	-		Dshi_3380	0.22	+0.0
3,553,588	-		Dshi_3380	0.22	+1.4
3,553,588	-		Dshi_3380	0.22	-0.4
3,553,594	+		Dshi_3380	0.23	+0.9
3,553,597	-		Dshi_3380	0.24	-0.7
3,553,602	+		Dshi_3380	0.24	-0.5
3,553,602	-		Dshi_3380	0.24	+1.0
3,553,617	+		Dshi_3380	0.26	+0.3
3,553,622	+		Dshi_3380	0.27	+0.4
3,553,625	+		Dshi_3380	0.27	+0.0
3,553,625	-		Dshi_3380	0.27	+1.4
3,553,626	-		Dshi_3380	0.27	-0.9
3,553,628	+		Dshi_3380	0.27	-0.4
3,553,630	-		Dshi_3380	0.28	-1.2
3,553,630	-		Dshi_3380	0.28	+0.1
3,553,630	-		Dshi_3380	0.28	-0.6
3,553,633	+		Dshi_3380	0.28	-0.4
3,553,633	+		Dshi_3380	0.28	-0.7
3,553,633	+		Dshi_3380	0.28	-0.5
3,553,638	+		Dshi_3380	0.29	+0.8
3,553,638	+		Dshi_3380	0.29	+0.7
3,553,638	+		Dshi_3380	0.29	-0.1
3,553,638	+		Dshi_3380	0.29	-1.9
3,553,639	+		Dshi_3380	0.29	-1.4
3,553,639	+		Dshi_3380	0.29	-0.4
3,553,639	+		Dshi_3380	0.29	-0.7
3,553,639	+		Dshi_3380	0.29	-0.0
3,553,639	+		Dshi_3380	0.29	-0.3
3,553,639	+		Dshi_3380	0.29	+0.3
3,553,641	-		Dshi_3380	0.29	-0.8
3,553,641	-		Dshi_3380	0.29	+0.3
3,553,643	-		Dshi_3380	0.29	+0.4
3,553,646	-		Dshi_3380	0.30	+0.5
3,553,646	-		Dshi_3380	0.30	-0.2
3,553,647	+		Dshi_3380	0.30	-1.3
3,553,647	+		Dshi_3380	0.30	+0.9
3,553,647	-		Dshi_3380	0.30	+1.0
3,553,647	-		Dshi_3380	0.30	+0.9
3,553,647	-		Dshi_3380	0.30	-0.6
3,553,682	+		Dshi_3380	0.34	+0.3
3,553,683	+		Dshi_3380	0.34	+0.3
3,553,689	+		Dshi_3380	0.35	+0.4
3,553,689	+		Dshi_3380	0.35	+0.2
3,553,691	-		Dshi_3380	0.35	-1.0
3,553,695	+		Dshi_3380	0.36	+0.3
3,553,695	+		Dshi_3380	0.36	+0.0
3,553,695	+		Dshi_3380	0.36	+0.0
3,553,704	+		Dshi_3380	0.37	+0.7
3,553,717	-		Dshi_3380	0.38	-1.3
3,553,746	-		Dshi_3380	0.42	-2.7
3,553,746	-		Dshi_3380	0.42	-0.5
3,553,746	-		Dshi_3380	0.42	+0.4
3,553,762	+		Dshi_3380	0.44	+0.0
3,553,763	+		Dshi_3380	0.44	+0.2
3,553,770	-		Dshi_3380	0.45	-0.8
3,553,777	-		Dshi_3380	0.46	+1.6
3,553,824	+		Dshi_3380	0.51	-0.5
3,553,834	+		Dshi_3380	0.53	-2.0
3,553,847	+		Dshi_3380	0.54	-1.5
3,553,847	-		Dshi_3380	0.54	+0.3
3,553,847	-		Dshi_3380	0.54	-0.2
3,553,849	+		Dshi_3380	0.54	-0.2
3,553,855	-		Dshi_3380	0.55	-0.2
3,553,857	-		Dshi_3380	0.55	-1.0
3,553,857	-		Dshi_3380	0.55	-0.2
3,553,857	-		Dshi_3380	0.55	+0.0
3,553,859	+		Dshi_3380	0.56	-0.3
3,553,869	-		Dshi_3380	0.57	+1.0
3,553,880	+		Dshi_3380	0.58	-0.2
3,553,885	+		Dshi_3380	0.59	+1.2
3,553,893	-		Dshi_3380	0.60	-1.7
3,553,893	-		Dshi_3380	0.60	-0.4
3,553,893	-		Dshi_3380	0.60	-1.0
3,553,895	-		Dshi_3380	0.60	-1.4
3,553,902	-		Dshi_3380	0.61	+0.8
3,553,902	-		Dshi_3380	0.61	-0.7
3,553,902	-		Dshi_3380	0.61	-0.5
3,553,904	+		Dshi_3380	0.61	+0.8
3,553,904	+		Dshi_3380	0.61	-0.4
3,553,904	+		Dshi_3380	0.61	-0.3
3,553,912	-		Dshi_3380	0.62	-0.5
3,553,912	-		Dshi_3380	0.62	+1.1
3,553,912	-		Dshi_3380	0.62	-0.8
3,553,912	-		Dshi_3380	0.62	+0.0
3,553,918	-		Dshi_3380	0.63	-0.8
3,553,968	+		Dshi_3380	0.69	-1.3
3,553,976	-		Dshi_3380	0.70	-1.9
3,553,978	+		Dshi_3380	0.70	+0.0
3,553,978	+		Dshi_3380	0.70	+0.5
3,553,983	+		Dshi_3380	0.71	-0.6
3,553,983	+		Dshi_3380	0.71	+0.5
3,553,986	-		Dshi_3380	0.71	+1.3
3,553,986	-		Dshi_3380	0.71	-0.3
3,553,986	-		Dshi_3380	0.71	+0.5
3,553,986	-		Dshi_3380	0.71	-0.7
3,553,986	-		Dshi_3380	0.71	-0.9
3,553,986	-		Dshi_3380	0.71	-0.6
3,553,986	-		Dshi_3380	0.71	+0.6
3,553,988	+		Dshi_3380	0.71	-0.2
3,553,988	-		Dshi_3380	0.71	+0.7
3,553,988	-		Dshi_3380	0.71	+0.1
3,553,991	-		Dshi_3380	0.72	+0.6
3,553,991	-		Dshi_3380	0.72	-0.6
3,554,030	+		Dshi_3380	0.76	+0.5
3,554,042	-		Dshi_3380	0.78	-0.9
3,554,058	-		Dshi_3380	0.80	-0.1
3,554,068	-		Dshi_3380	0.81	-0.3
3,554,083	+		Dshi_3380	0.83	+0.5
3,554,091	-		Dshi_3380	0.84	-1.0
3,554,096	-		Dshi_3380	0.84	-0.1
3,554,118	+		Dshi_3380	0.87	-0.8
3,554,126	+		Dshi_3380	0.88	-0.4
3,554,126	-		Dshi_3380	0.88	+1.5
3,554,149	+				+0.5
3,554,149	+				-0.1
3,554,149	+				+0.7
3,554,149	+				-0.3
3,554,149	+				-0.3
3,554,149	+				+0.3
3,554,149	+				-0.6
3,554,149	+				-0.2
3,554,151	+				-0.6
3,554,151	+				-0.4
3,554,154	+				-1.3
3,554,157	-				-0.7
3,554,157	-				-0.1
3,554,157	-				+0.7
3,554,157	-				-0.5
3,554,157	-				-1.0
3,554,159	-				-0.1
3,554,201	+				-0.7
3,554,201	+				-0.6
3,554,209	-				-0.5
3,554,209	-				-1.4
3,554,214	-				+1.5
3,554,220	+				-0.1
3,554,230	+				+0.5
3,554,230	+				+0.5
3,554,230	+				+1.1
3,554,230	+				+0.4
3,554,230	+				+0.2
3,554,230	+				-0.9
3,554,230	+				-0.2
3,554,230	+				-0.4
3,554,230	+				-0.3
3,554,230	+				-0.3
3,554,238	-				-0.3
3,554,238	-				-0.6
3,554,238	-				+1.7
3,554,238	-				-0.6
3,554,238	-				-2.4
3,554,238	-				-0.1
3,554,238	-				-0.3
3,554,238	-				-0.8
3,554,238	-				-2.1
3,554,238	-				+0.5
3,554,247	+				+0.7
3,554,254	+				+0.3
3,554,255	-				-0.1
3,554,255	-				+0.6
3,554,260	-				-0.8
3,554,262	-				-0.0
3,554,262	-				-1.4
3,554,262	-				-0.1
3,554,268	+				+0.3
3,554,272	-				+1.3
3,554,276	-				-0.0
3,554,287	+				-0.6
3,554,324	+				-0.7
3,554,324	+				-0.3
3,554,324	+				-1.8
3,554,324	+				-0.3
3,554,324	+				+0.1
3,554,324	+				-0.2
3,554,324	+				+2.5
3,554,324	+				-0.3
3,554,324	+				+2.0
3,554,332	-				-0.1
3,554,332	-				-0.3
3,554,351	+				+0.4
3,554,366	+				-2.4
3,554,369	-				+0.0
3,554,374	-				-0.4
3,554,383	+				-0.0
3,554,391	-				-0.2
3,554,391	-				-0.8
3,554,391	-				-1.2
3,554,391	-				-0.7
3,554,397	-				+0.5
3,554,398	-				-1.4
3,554,413	-				-1.3
3,554,415	-				-1.7
3,554,464	+				-1.0
3,554,472	-				-0.5
3,554,483	+				-1.1
3,554,515	+				+1.1
3,554,540	+				-2.6
3,554,548	-				-1.2
3,554,574	+				-0.5
3,554,574	+				+0.5
3,554,641	-		Dshi_3381	0.13	-1.1
3,554,644	+		Dshi_3381	0.13	+0.9
3,554,644	+		Dshi_3381	0.13	+0.9
3,554,644	+		Dshi_3381	0.13	-1.6
3,554,644	+		Dshi_3381	0.13	+2.5
3,554,644	+		Dshi_3381	0.13	-0.8
3,554,645	+		Dshi_3381	0.13	-1.1
3,554,645	+		Dshi_3381	0.13	+2.5
3,554,645	+		Dshi_3381	0.13	-1.7
3,554,645	+		Dshi_3381	0.13	+0.2
3,554,645	+		Dshi_3381	0.13	-1.4
3,554,653	-		Dshi_3381	0.14	-1.2
3,554,653	-		Dshi_3381	0.14	-0.8
3,554,653	-		Dshi_3381	0.14	-1.5
3,554,653	-		Dshi_3381	0.14	-0.5
3,554,653	-		Dshi_3381	0.14	-0.7
3,554,661	-		Dshi_3381	0.14	-0.1
3,554,715	+		Dshi_3381	0.17	-0.8
3,554,759	+		Dshi_3381	0.20	-1.9
3,554,778	+		Dshi_3381	0.21	+1.5
3,554,778	+		Dshi_3381	0.21	-1.5
3,554,783	+		Dshi_3381	0.21	-0.7
3,554,820	-		Dshi_3381	0.23	-1.6
3,554,824	+		Dshi_3381	0.23	-0.5
3,554,824	+		Dshi_3381	0.23	-1.5
3,554,838	+		Dshi_3381	0.24	-0.7
3,554,882	+		Dshi_3381	0.27	-0.3
3,554,887	+		Dshi_3381	0.27	-0.7
3,554,887	+		Dshi_3381	0.27	-0.3
3,554,887	+		Dshi_3381	0.27	-3.4
3,554,887	+		Dshi_3381	0.27	-0.3
3,554,890	-		Dshi_3381	0.27	+1.5
3,554,895	-		Dshi_3381	0.27	+1.5
3,554,895	-		Dshi_3381	0.27	-1.8
3,554,924	+		Dshi_3381	0.29	+0.9
3,554,948	-		Dshi_3381	0.30	-2.4
3,554,960	+		Dshi_3381	0.31	-1.9
3,554,960	+		Dshi_3381	0.31	+2.5
3,554,963	-		Dshi_3381	0.31	-1.7
3,554,968	-		Dshi_3381	0.31	-1.5
3,555,011	-		Dshi_3381	0.34	-0.7
3,555,036	-		Dshi_3381	0.35	+2.5
3,555,045	-		Dshi_3381	0.36	+0.9
3,555,061	-		Dshi_3381	0.37	+0.7
3,555,067	-		Dshi_3381	0.37	+0.5
3,555,103	+		Dshi_3381	0.39	-1.0
3,555,113	+		Dshi_3381	0.39	-0.8
3,555,140	-		Dshi_3381	0.41	-0.1
3,555,140	-		Dshi_3381	0.41	-1.5
3,555,194	+		Dshi_3381	0.44	+2.5
3,555,202	-		Dshi_3381	0.44	-1.7
3,555,218	+		Dshi_3381	0.45	-0.8