Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09250 and PGA1_c09260 are separated by 225 nucleotides PGA1_c09260 and PGA1_c09270 are separated by 144 nucleotides PGA1_c09270 and PGA1_c09280 are separated by 132 nucleotides PGA1_c09280 and PGA1_c09290 are separated by 104 nucleotides
PGA1_c09250: PGA1_c09250 - Protein of unknown function (DUF2867)., at 948,015 to 948,497
_c09250
PGA1_c09260: PGA1_c09260 - Uncharacterized conserved protein, at 948,723 to 949,169
_c09260
PGA1_c09270: PGA1_c09270 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HisA, at 949,314 to 950,036
_c09270
PGA1_c09280: PGA1_c09280 - Uncharacterized protein conserved in bacteria, at 950,169 to 950,555
_c09280
PGA1_c09290: PGA1_c09290 - WGR domain., at 950,660 to 950,911
_c09290
Position (kb) 949
950
951 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 948.344 kb on - strand, within PGA1_c09250 at 948.436 kb on + strand, within PGA1_c09250 at 948.510 kb on + strand at 948.516 kb on - strand at 948.523 kb on - strand at 948.532 kb on + strand at 948.532 kb on + strand at 948.540 kb on - strand at 948.551 kb on - strand at 948.593 kb on + strand at 948.616 kb on + strand at 948.618 kb on - strand at 948.618 kb on - strand at 948.624 kb on - strand at 948.635 kb on + strand at 948.662 kb on + strand at 948.669 kb on + strand at 948.669 kb on + strand at 948.669 kb on + strand at 948.669 kb on + strand at 948.674 kb on + strand at 948.674 kb on + strand at 948.674 kb on + strand at 948.674 kb on + strand at 948.677 kb on - strand at 948.677 kb on - strand at 948.677 kb on - strand at 948.677 kb on - strand at 948.679 kb on + strand at 948.679 kb on + strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.707 kb on + strand at 948.759 kb on + strand at 948.781 kb on + strand, within PGA1_c09260 at 948.781 kb on + strand, within PGA1_c09260 at 948.845 kb on - strand, within PGA1_c09260 at 948.870 kb on + strand, within PGA1_c09260 at 948.870 kb on + strand, within PGA1_c09260 at 948.975 kb on - strand, within PGA1_c09260 at 948.975 kb on - strand, within PGA1_c09260 at 949.091 kb on + strand, within PGA1_c09260 at 949.153 kb on - strand at 949.235 kb on - strand at 949.282 kb on + strand at 949.285 kb on - strand at 949.290 kb on - strand at 949.329 kb on + strand at 950.117 kb on + strand at 950.195 kb on - strand at 950.213 kb on - strand, within PGA1_c09280 at 950.214 kb on + strand, within PGA1_c09280 at 950.222 kb on - strand, within PGA1_c09280 at 950.227 kb on - strand, within PGA1_c09280 at 950.277 kb on - strand, within PGA1_c09280 at 950.294 kb on + strand, within PGA1_c09280 at 950.297 kb on + strand, within PGA1_c09280 at 950.310 kb on - strand, within PGA1_c09280 at 950.343 kb on + strand, within PGA1_c09280 at 950.421 kb on + strand, within PGA1_c09280 at 950.426 kb on + strand, within PGA1_c09280 at 950.435 kb on - strand, within PGA1_c09280 at 950.444 kb on - strand, within PGA1_c09280 at 950.464 kb on + strand, within PGA1_c09280 at 950.465 kb on + strand, within PGA1_c09280 at 950.514 kb on + strand, within PGA1_c09280 at 950.522 kb on - strand at 950.532 kb on - strand at 950.532 kb on - strand at 950.601 kb on + strand at 950.606 kb on + strand at 950.658 kb on + strand at 950.666 kb on - strand at 950.666 kb on - strand at 950.666 kb on - strand at 950.781 kb on + strand, within PGA1_c09290 at 950.802 kb on + strand, within PGA1_c09290 at 950.802 kb on + strand, within PGA1_c09290 at 950.810 kb on - strand, within PGA1_c09290 at 950.812 kb on + strand, within PGA1_c09290 at 950.859 kb on + strand, within PGA1_c09290 at 950.859 kb on + strand, within PGA1_c09290 at 950.903 kb on + strand at 950.903 kb on + strand at 950.903 kb on + strand at 950.903 kb on + strand at 950.911 kb on - strand at 950.911 kb on - strand at 950.911 kb on - strand at 950.911 kb on - strand at 950.954 kb on + strand at 950.954 kb on + strand at 950.954 kb on - strand at 950.962 kb on - strand at 950.962 kb on - strand at 950.962 kb on - strand at 950.962 kb on - strand at 950.962 kb on - strand at 950.969 kb on + strand at 951.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 948,344 - PGA1_c09250 0.68 -1.1 948,436 + PGA1_c09250 0.87 +0.1 948,510 + -0.6 948,516 - -0.5 948,523 - -0.1 948,532 + -0.9 948,532 + -2.7 948,540 - -1.9 948,551 - -0.5 948,593 + +0.1 948,616 + -0.4 948,618 - -0.1 948,618 - -0.2 948,624 - -1.2 948,635 + +0.4 948,662 + +0.0 948,669 + +0.6 948,669 + +0.4 948,669 + +0.2 948,669 + +0.1 948,674 + -0.6 948,674 + +0.9 948,674 + +1.0 948,674 + +0.4 948,677 - -2.9 948,677 - +1.6 948,677 - -0.1 948,677 - +0.6 948,679 + -0.9 948,679 + +2.2 948,682 - -2.1 948,682 - -0.5 948,682 - -0.8 948,707 + +0.6 948,759 + -2.1 948,781 + PGA1_c09260 0.13 +1.2 948,781 + PGA1_c09260 0.13 +1.4 948,845 - PGA1_c09260 0.27 -2.1 948,870 + PGA1_c09260 0.33 -1.8 948,870 + PGA1_c09260 0.33 +2.5 948,975 - PGA1_c09260 0.56 +2.7 948,975 - PGA1_c09260 0.56 +1.1 949,091 + PGA1_c09260 0.82 -0.9 949,153 - -1.1 949,235 - +0.8 949,282 + -0.5 949,285 - -1.1 949,290 - +1.2 949,329 + -1.1 950,117 + +0.2 950,195 - +0.2 950,213 - PGA1_c09280 0.11 +0.3 950,214 + PGA1_c09280 0.12 -1.7 950,222 - PGA1_c09280 0.14 -0.6 950,227 - PGA1_c09280 0.15 -2.3 950,277 - PGA1_c09280 0.28 +0.2 950,294 + PGA1_c09280 0.32 +0.4 950,297 + PGA1_c09280 0.33 +0.7 950,310 - PGA1_c09280 0.36 +1.2 950,343 + PGA1_c09280 0.45 +0.4 950,421 + PGA1_c09280 0.65 +0.6 950,426 + PGA1_c09280 0.66 +0.7 950,435 - PGA1_c09280 0.69 -0.0 950,444 - PGA1_c09280 0.71 +0.9 950,464 + PGA1_c09280 0.76 -0.2 950,465 + PGA1_c09280 0.76 +1.7 950,514 + PGA1_c09280 0.89 -0.1 950,522 - -0.4 950,532 - +1.0 950,532 - +0.5 950,601 + +1.0 950,606 + +0.9 950,658 + -1.3 950,666 - -1.2 950,666 - +0.1 950,666 - -1.3 950,781 + PGA1_c09290 0.48 -0.3 950,802 + PGA1_c09290 0.56 +1.7 950,802 + PGA1_c09290 0.56 +2.2 950,810 - PGA1_c09290 0.60 -0.3 950,812 + PGA1_c09290 0.60 +1.2 950,859 + PGA1_c09290 0.79 -0.9 950,859 + PGA1_c09290 0.79 +1.1 950,903 + +0.7 950,903 + -0.3 950,903 + -0.1 950,903 + +1.0 950,911 - +0.0 950,911 - +1.4 950,911 - +1.7 950,911 - +0.9 950,954 + +0.5 950,954 + +0.8 950,954 - -1.8 950,962 - +0.1 950,962 - -2.3 950,962 - +0.0 950,962 - -2.3 950,962 - -0.1 950,969 + +0.7 951,032 - -0.1
Or see this region's nucleotide sequence