Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20210 and PGA1_c20220 are separated by 66 nucleotides PGA1_c20220 and PGA1_c20230 are separated by 127 nucleotides PGA1_c20230 and PGA1_c20240 are separated by 219 nucleotides
PGA1_c20210: PGA1_c20210 - acetolactate synthase isozyme 3 large subunit, at 2,097,059 to 2,098,810
_c20210
PGA1_c20220: PGA1_c20220 - acetolactate synthase isozyme 3 small subunit, at 2,098,877 to 2,099,437
_c20220
PGA1_c20230: PGA1_c20230 - hypothetical protein, at 2,099,565 to 2,100,047
_c20230
PGA1_c20240: PGA1_c20240 - Helix-turn-helix., at 2,100,267 to 2,101,073
_c20240
Position (kb)
2099
2100
2101 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2098.603 kb on + strand, within PGA1_c20210 at 2098.628 kb on + strand, within PGA1_c20210 at 2098.628 kb on + strand, within PGA1_c20210 at 2098.636 kb on - strand at 2098.804 kb on + strand at 2098.804 kb on + strand at 2098.804 kb on + strand at 2098.804 kb on + strand at 2098.812 kb on - strand at 2098.835 kb on - strand at 2098.879 kb on - strand at 2098.891 kb on - strand at 2098.958 kb on + strand, within PGA1_c20220 at 2098.966 kb on - strand, within PGA1_c20220 at 2099.005 kb on - strand, within PGA1_c20220 at 2099.049 kb on + strand, within PGA1_c20220 at 2099.123 kb on - strand, within PGA1_c20220 at 2099.123 kb on - strand, within PGA1_c20220 at 2099.132 kb on - strand, within PGA1_c20220 at 2099.134 kb on + strand, within PGA1_c20220 at 2099.142 kb on - strand, within PGA1_c20220 at 2099.181 kb on + strand, within PGA1_c20220 at 2099.199 kb on + strand, within PGA1_c20220 at 2099.379 kb on + strand, within PGA1_c20220 at 2099.422 kb on + strand at 2099.436 kb on + strand at 2099.457 kb on + strand at 2099.492 kb on + strand at 2099.492 kb on + strand at 2099.517 kb on - strand at 2099.603 kb on - strand at 2099.673 kb on - strand, within PGA1_c20230 at 2099.687 kb on + strand, within PGA1_c20230 at 2099.721 kb on + strand, within PGA1_c20230 at 2099.812 kb on + strand, within PGA1_c20230 at 2099.812 kb on + strand, within PGA1_c20230 at 2099.820 kb on - strand, within PGA1_c20230 at 2099.820 kb on - strand, within PGA1_c20230 at 2099.846 kb on + strand, within PGA1_c20230 at 2099.875 kb on - strand, within PGA1_c20230 at 2099.923 kb on + strand, within PGA1_c20230 at 2099.945 kb on + strand, within PGA1_c20230 at 2099.970 kb on + strand, within PGA1_c20230 at 2099.978 kb on - strand, within PGA1_c20230 at 2099.992 kb on + strand, within PGA1_c20230 at 2100.022 kb on - strand at 2100.047 kb on + strand at 2100.059 kb on + strand at 2100.059 kb on + strand at 2100.059 kb on + strand at 2100.059 kb on + strand at 2100.059 kb on + strand at 2100.067 kb on - strand at 2100.067 kb on - strand at 2100.067 kb on - strand at 2100.258 kb on - strand at 2100.258 kb on - strand at 2100.282 kb on - strand at 2100.322 kb on + strand at 2100.349 kb on - strand, within PGA1_c20240 at 2100.384 kb on + strand, within PGA1_c20240 at 2100.409 kb on - strand, within PGA1_c20240 at 2100.414 kb on - strand, within PGA1_c20240 at 2100.414 kb on - strand, within PGA1_c20240 at 2100.420 kb on + strand, within PGA1_c20240 at 2100.435 kb on - strand, within PGA1_c20240 at 2100.444 kb on + strand, within PGA1_c20240 at 2100.497 kb on - strand, within PGA1_c20240 at 2100.522 kb on + strand, within PGA1_c20240 at 2100.562 kb on + strand, within PGA1_c20240 at 2100.612 kb on - strand, within PGA1_c20240 at 2100.612 kb on - strand, within PGA1_c20240 at 2100.637 kb on - strand, within PGA1_c20240 at 2100.654 kb on - strand, within PGA1_c20240 at 2100.684 kb on + strand, within PGA1_c20240 at 2100.692 kb on - strand, within PGA1_c20240 at 2100.730 kb on + strand, within PGA1_c20240 at 2100.737 kb on + strand, within PGA1_c20240 at 2100.746 kb on - strand, within PGA1_c20240 at 2100.776 kb on + strand, within PGA1_c20240 at 2100.801 kb on + strand, within PGA1_c20240 at 2100.809 kb on - strand, within PGA1_c20240 at 2100.878 kb on + strand, within PGA1_c20240 at 2100.945 kb on - strand, within PGA1_c20240 at 2100.947 kb on - strand, within PGA1_c20240 at 2100.981 kb on - strand, within PGA1_c20240 at 2100.983 kb on - strand, within PGA1_c20240 at 2100.997 kb on + strand at 2101.030 kb on + strand at 2101.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,098,603 + PGA1_c20210 0.88 +0.0 2,098,628 + PGA1_c20210 0.90 -1.8 2,098,628 + PGA1_c20210 0.90 -1.8 2,098,636 - -1.8 2,098,804 + +0.6 2,098,804 + +1.3 2,098,804 + +0.6 2,098,804 + +1.0 2,098,812 - -1.1 2,098,835 - +0.8 2,098,879 - -0.5 2,098,891 - +0.2 2,098,958 + PGA1_c20220 0.14 +1.0 2,098,966 - PGA1_c20220 0.16 +0.6 2,099,005 - PGA1_c20220 0.23 -1.8 2,099,049 + PGA1_c20220 0.31 -0.2 2,099,123 - PGA1_c20220 0.44 +0.3 2,099,123 - PGA1_c20220 0.44 -1.5 2,099,132 - PGA1_c20220 0.45 -1.5 2,099,134 + PGA1_c20220 0.46 -1.4 2,099,142 - PGA1_c20220 0.47 -1.9 2,099,181 + PGA1_c20220 0.54 -0.7 2,099,199 + PGA1_c20220 0.57 -0.5 2,099,379 + PGA1_c20220 0.89 +0.0 2,099,422 + +0.6 2,099,436 + -0.4 2,099,457 + +0.9 2,099,492 + -0.5 2,099,492 + +0.6 2,099,517 - +0.7 2,099,603 - -0.7 2,099,673 - PGA1_c20230 0.22 -0.1 2,099,687 + PGA1_c20230 0.25 -0.8 2,099,721 + PGA1_c20230 0.32 -0.3 2,099,812 + PGA1_c20230 0.51 -0.2 2,099,812 + PGA1_c20230 0.51 -0.5 2,099,820 - PGA1_c20230 0.53 -0.7 2,099,820 - PGA1_c20230 0.53 -0.4 2,099,846 + PGA1_c20230 0.58 +0.6 2,099,875 - PGA1_c20230 0.64 -1.1 2,099,923 + PGA1_c20230 0.74 +1.2 2,099,945 + PGA1_c20230 0.79 +0.9 2,099,970 + PGA1_c20230 0.84 +0.0 2,099,978 - PGA1_c20230 0.86 +1.5 2,099,992 + PGA1_c20230 0.88 -1.4 2,100,022 - +0.6 2,100,047 + +0.6 2,100,059 + +0.2 2,100,059 + +0.6 2,100,059 + +1.0 2,100,059 + +0.6 2,100,059 + -0.1 2,100,067 - +0.5 2,100,067 - -0.2 2,100,067 - -0.2 2,100,258 - -0.2 2,100,258 - -0.5 2,100,282 - +0.7 2,100,322 + -0.2 2,100,349 - PGA1_c20240 0.10 +0.4 2,100,384 + PGA1_c20240 0.14 +0.8 2,100,409 - PGA1_c20240 0.18 -0.7 2,100,414 - PGA1_c20240 0.18 -1.2 2,100,414 - PGA1_c20240 0.18 -2.1 2,100,420 + PGA1_c20240 0.19 -1.1 2,100,435 - PGA1_c20240 0.21 +2.0 2,100,444 + PGA1_c20240 0.22 -0.5 2,100,497 - PGA1_c20240 0.29 -0.3 2,100,522 + PGA1_c20240 0.32 -0.5 2,100,562 + PGA1_c20240 0.37 -0.5 2,100,612 - PGA1_c20240 0.43 +1.0 2,100,612 - PGA1_c20240 0.43 +0.7 2,100,637 - PGA1_c20240 0.46 -1.1 2,100,654 - PGA1_c20240 0.48 -0.8 2,100,684 + PGA1_c20240 0.52 +0.5 2,100,692 - PGA1_c20240 0.53 -2.3 2,100,730 + PGA1_c20240 0.57 -1.4 2,100,737 + PGA1_c20240 0.58 -1.4 2,100,746 - PGA1_c20240 0.59 -0.7 2,100,776 + PGA1_c20240 0.63 +0.3 2,100,801 + PGA1_c20240 0.66 +1.7 2,100,809 - PGA1_c20240 0.67 -0.7 2,100,878 + PGA1_c20240 0.76 +0.7 2,100,945 - PGA1_c20240 0.84 -0.8 2,100,947 - PGA1_c20240 0.84 -0.0 2,100,981 - PGA1_c20240 0.88 +3.3 2,100,983 - PGA1_c20240 0.89 -1.2 2,100,997 + +2.6 2,101,030 + +1.3 2,101,038 - +0.7
Or see this region's nucleotide sequence