Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01010 and PGA1_c01020 are separated by 33 nucleotides PGA1_c01020 and PGA1_c01030 are separated by 99 nucleotides PGA1_c01030 and PGA1_c01040 are separated by 59 nucleotides PGA1_c01040 and PGA1_c01050 are separated by 63 nucleotides
PGA1_c01010: PGA1_c01010 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase SpoT, at 94,762 to 96,894
_c01010
PGA1_c01020: PGA1_c01020 - Uncharacterized protein conserved in bacteria, at 96,928 to 97,581
_c01020
PGA1_c01030: PGA1_c01030 - pyridoxine 5'-phosphate synthase PdxJ, at 97,681 to 98,442
_c01030
PGA1_c01040: PGA1_c01040 - holo-[acyl-carrier-protein] synthase AcpS, at 98,502 to 98,933
_c01040
PGA1_c01050: PGA1_c01050 - putative signal peptidase I, at 98,997 to 99,833
_c01050
Position (kb) 97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 96.681 kb on - strand at 96.681 kb on - strand at 96.681 kb on - strand at 96.684 kb on + strand at 96.684 kb on + strand at 96.692 kb on - strand at 96.727 kb on - strand at 96.858 kb on - strand at 96.858 kb on - strand at 96.858 kb on - strand at 97.487 kb on + strand, within PGA1_c01020 at 97.559 kb on + strand at 97.575 kb on + strand at 97.621 kb on + strand at 97.621 kb on + strand at 97.629 kb on - strand at 97.631 kb on + strand at 97.642 kb on + strand at 98.481 kb on - strand at 98.488 kb on + strand at 98.491 kb on - strand at 98.496 kb on - strand at 98.496 kb on - strand at 98.496 kb on - strand at 98.498 kb on + strand at 98.505 kb on + strand at 98.513 kb on + strand at 98.554 kb on + strand, within PGA1_c01040 at 98.599 kb on + strand, within PGA1_c01040 at 98.607 kb on - strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.616 kb on + strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.654 kb on + strand, within PGA1_c01040 at 98.685 kb on - strand, within PGA1_c01040 at 98.697 kb on + strand, within PGA1_c01040 at 98.697 kb on + strand, within PGA1_c01040 at 98.800 kb on + strand, within PGA1_c01040 at 98.855 kb on + strand, within PGA1_c01040 at 98.855 kb on + strand, within PGA1_c01040 at 98.855 kb on + strand, within PGA1_c01040 at 98.863 kb on - strand, within PGA1_c01040 at 98.867 kb on + strand, within PGA1_c01040 at 98.875 kb on - strand, within PGA1_c01040
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 96,681 - -0.7 96,681 - -0.9 96,681 - -1.0 96,684 + -0.9 96,684 + -1.6 96,692 - -3.4 96,727 - -1.3 96,858 - +1.1 96,858 - -0.6 96,858 - -1.0 97,487 + PGA1_c01020 0.85 -2.4 97,559 + -0.4 97,575 + -0.4 97,621 + -1.9 97,621 + -0.5 97,629 - -0.4 97,631 + +0.4 97,642 + +0.4 98,481 - -1.5 98,488 + -0.1 98,491 - +0.8 98,496 - -2.3 98,496 - -0.5 98,496 - -0.9 98,498 + -0.6 98,505 + -1.4 98,513 + +0.4 98,554 + PGA1_c01040 0.12 -0.2 98,599 + PGA1_c01040 0.22 +0.1 98,607 - PGA1_c01040 0.24 +0.9 98,609 + PGA1_c01040 0.25 -0.1 98,609 + PGA1_c01040 0.25 +0.3 98,609 + PGA1_c01040 0.25 +1.4 98,609 + PGA1_c01040 0.25 +0.2 98,609 + PGA1_c01040 0.25 -0.6 98,609 + PGA1_c01040 0.25 +0.5 98,609 + PGA1_c01040 0.25 -0.7 98,609 + PGA1_c01040 0.25 -0.4 98,609 + PGA1_c01040 0.25 +0.3 98,609 + PGA1_c01040 0.25 +0.0 98,609 + PGA1_c01040 0.25 -1.4 98,609 + PGA1_c01040 0.25 +0.4 98,609 + PGA1_c01040 0.25 +0.3 98,616 + PGA1_c01040 0.26 -0.3 98,617 - PGA1_c01040 0.27 -0.4 98,617 - PGA1_c01040 0.27 -0.8 98,617 - PGA1_c01040 0.27 +0.7 98,617 - PGA1_c01040 0.27 +1.3 98,617 - PGA1_c01040 0.27 +0.4 98,617 - PGA1_c01040 0.27 +0.1 98,617 - PGA1_c01040 0.27 +1.2 98,617 - PGA1_c01040 0.27 -0.7 98,617 - PGA1_c01040 0.27 -0.6 98,617 - PGA1_c01040 0.27 +0.6 98,617 - PGA1_c01040 0.27 +1.4 98,617 - PGA1_c01040 0.27 +0.3 98,617 - PGA1_c01040 0.27 -0.4 98,617 - PGA1_c01040 0.27 -0.9 98,617 - PGA1_c01040 0.27 +0.2 98,617 - PGA1_c01040 0.27 +0.1 98,617 - PGA1_c01040 0.27 -1.0 98,617 - PGA1_c01040 0.27 +0.6 98,654 + PGA1_c01040 0.35 -0.0 98,685 - PGA1_c01040 0.42 -0.6 98,697 + PGA1_c01040 0.45 -0.0 98,697 + PGA1_c01040 0.45 +0.1 98,800 + PGA1_c01040 0.69 +2.0 98,855 + PGA1_c01040 0.82 +0.1 98,855 + PGA1_c01040 0.82 +0.8 98,855 + PGA1_c01040 0.82 +0.8 98,863 - PGA1_c01040 0.84 -0.1 98,867 + PGA1_c01040 0.84 +0.4 98,875 - PGA1_c01040 0.86 -1.7
Or see this region's nucleotide sequence