Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0025 and Dshi_0026 are separated by 73 nucleotides Dshi_0026 and Dshi_0027 are separated by 123 nucleotides Dshi_0027 and Dshi_0028 are separated by 7 nucleotides
Dshi_0025: Dshi_0025 - anti-ECFsigma factor, ChrR (RefSeq), at 29,143 to 29,814
_0025
Dshi_0026: Dshi_0026 - NAD-dependent epimerase/dehydratase (RefSeq), at 29,888 to 30,529
_0026
Dshi_0027: Dshi_0027 - Alkane 1-monooxygenase (RefSeq), at 30,653 to 31,807
_0027
Dshi_0028: Dshi_0028 - A/G-specific adenine glycosylase (RefSeq), at 31,815 to 32,909
_0028
Position (kb) 30
31
32 Strain fitness (log2 ratio) -2
-1
0
1
2 at 29.667 kb on + strand, within Dshi_0025 at 29.723 kb on + strand, within Dshi_0025 at 29.731 kb on - strand at 29.821 kb on - strand at 29.822 kb on - strand at 29.838 kb on - strand at 29.996 kb on + strand, within Dshi_0026 at 30.039 kb on - strand, within Dshi_0026 at 30.258 kb on - strand, within Dshi_0026 at 30.314 kb on + strand, within Dshi_0026 at 30.337 kb on - strand, within Dshi_0026 at 30.341 kb on + strand, within Dshi_0026 at 30.341 kb on + strand, within Dshi_0026 at 30.351 kb on + strand, within Dshi_0026 at 30.395 kb on + strand, within Dshi_0026 at 30.403 kb on - strand, within Dshi_0026 at 30.419 kb on + strand, within Dshi_0026 at 30.703 kb on - strand at 30.756 kb on + strand at 30.780 kb on + strand, within Dshi_0027 at 30.811 kb on + strand, within Dshi_0027 at 30.821 kb on + strand, within Dshi_0027 at 30.836 kb on + strand, within Dshi_0027 at 30.837 kb on + strand, within Dshi_0027 at 30.857 kb on - strand, within Dshi_0027 at 30.920 kb on + strand, within Dshi_0027 at 30.982 kb on - strand, within Dshi_0027 at 30.982 kb on - strand, within Dshi_0027 at 31.019 kb on - strand, within Dshi_0027 at 31.102 kb on - strand, within Dshi_0027 at 31.145 kb on + strand, within Dshi_0027 at 31.192 kb on + strand, within Dshi_0027 at 31.197 kb on + strand, within Dshi_0027 at 31.234 kb on + strand, within Dshi_0027 at 31.308 kb on + strand, within Dshi_0027 at 31.335 kb on + strand, within Dshi_0027 at 31.343 kb on - strand, within Dshi_0027 at 31.350 kb on + strand, within Dshi_0027 at 31.377 kb on - strand, within Dshi_0027 at 31.611 kb on + strand, within Dshi_0027 at 31.803 kb on + strand at 31.847 kb on - strand at 31.868 kb on - strand at 31.868 kb on - strand at 31.959 kb on + strand, within Dshi_0028 at 32.097 kb on + strand, within Dshi_0028 at 32.166 kb on - strand, within Dshi_0028 at 32.166 kb on - strand, within Dshi_0028 at 32.249 kb on + strand, within Dshi_0028 at 32.376 kb on - strand, within Dshi_0028 at 32.520 kb on - strand, within Dshi_0028 at 32.629 kb on + strand, within Dshi_0028 at 32.678 kb on + strand, within Dshi_0028
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 29,667 + Dshi_0025 0.78 -0.9 29,723 + Dshi_0025 0.86 +1.2 29,731 - +0.2 29,821 - +0.7 29,822 - -0.5 29,838 - -1.1 29,996 + Dshi_0026 0.17 -1.1 30,039 - Dshi_0026 0.24 +0.9 30,258 - Dshi_0026 0.58 +1.5 30,314 + Dshi_0026 0.66 -1.0 30,337 - Dshi_0026 0.70 +0.3 30,341 + Dshi_0026 0.71 -0.0 30,341 + Dshi_0026 0.71 +1.0 30,351 + Dshi_0026 0.72 -0.2 30,395 + Dshi_0026 0.79 -0.0 30,403 - Dshi_0026 0.80 -0.1 30,419 + Dshi_0026 0.83 -0.5 30,703 - -0.4 30,756 + -0.7 30,780 + Dshi_0027 0.11 -0.2 30,811 + Dshi_0027 0.14 +0.4 30,821 + Dshi_0027 0.15 +0.1 30,836 + Dshi_0027 0.16 +0.2 30,837 + Dshi_0027 0.16 +2.1 30,857 - Dshi_0027 0.18 -0.5 30,920 + Dshi_0027 0.23 +0.1 30,982 - Dshi_0027 0.28 -0.6 30,982 - Dshi_0027 0.28 -2.5 31,019 - Dshi_0027 0.32 +0.5 31,102 - Dshi_0027 0.39 -0.6 31,145 + Dshi_0027 0.43 +0.4 31,192 + Dshi_0027 0.47 -2.3 31,197 + Dshi_0027 0.47 -0.0 31,234 + Dshi_0027 0.50 +0.5 31,308 + Dshi_0027 0.57 +0.4 31,335 + Dshi_0027 0.59 -0.3 31,343 - Dshi_0027 0.60 -1.2 31,350 + Dshi_0027 0.60 +0.0 31,377 - Dshi_0027 0.63 -0.0 31,611 + Dshi_0027 0.83 -0.2 31,803 + +1.1 31,847 - -0.9 31,868 - -0.8 31,868 - +0.5 31,959 + Dshi_0028 0.13 +1.0 32,097 + Dshi_0028 0.26 -0.2 32,166 - Dshi_0028 0.32 +0.6 32,166 - Dshi_0028 0.32 +0.3 32,249 + Dshi_0028 0.40 +0.2 32,376 - Dshi_0028 0.51 +0.0 32,520 - Dshi_0028 0.64 -0.6 32,629 + Dshi_0028 0.74 +0.3 32,678 + Dshi_0028 0.79 +1.7
Or see this region's nucleotide sequence