Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0923 and ileS are separated by 42 nucleotides ileS and lspA are separated by 8 nucleotides lspA and Sama_0926 are separated by 3 nucleotides Sama_0926 and ispH overlap by 1 nucleotides
Sama_0923: Sama_0923 - riboflavin biosynthesis protein RibF (RefSeq), at 1,115,436 to 1,116,371
_0923
Sama_0924: ileS - isoleucyl-tRNA synthetase (RefSeq), at 1,116,414 to 1,119,236
ileS
Sama_0925: lspA - lipoprotein signal peptidase (RefSeq), at 1,119,245 to 1,119,751
lspA
Sama_0926: Sama_0926 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 1,119,755 to 1,120,174
_0926
Sama_0927: ispH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (RefSeq), at 1,120,174 to 1,121,115
ispH
Position (kb) 1116
1117
1118
1119
1120 Strain fitness (log2 ratio) -1
0
1 at 1115.416 kb on + strand at 1119.697 kb on + strand, within lspA at 1119.697 kb on + strand, within lspA at 1119.697 kb on + strand, within lspA at 1119.700 kb on + strand, within lspA at 1119.712 kb on + strand at 1119.712 kb on + strand at 1119.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 1,115,416 + -1.7 1,119,697 + lspA Sama_0925 0.89 +1.0 1,119,697 + lspA Sama_0925 0.89 +0.9 1,119,697 + lspA Sama_0925 0.89 +1.3 1,119,700 + lspA Sama_0925 0.90 +0.9 1,119,712 + -0.3 1,119,712 + -0.6 1,119,749 + +0.5
Or see this region's nucleotide sequence