Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3982 and Dshi_3983 are separated by 4 nucleotides Dshi_3983 and Dshi_3984 are separated by 187 nucleotides Dshi_3984 and Dshi_3985 are separated by 12 nucleotides
Dshi_3982: Dshi_3982 - conjugation TrbI family protein (RefSeq), at 40,545 to 41,924
_3982
Dshi_3983: Dshi_3983 - type II secretion system protein E (RefSeq), at 41,929 to 42,915
_3983
Dshi_3984: Dshi_3984 - hypothetical protein (RefSeq), at 43,103 to 43,513
_3984
Dshi_3985: Dshi_3985 - TrbL/VirB6 plasmid conjugal transfer protein (RefSeq), at 43,526 to 44,602
_3985
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 41.656 kb on + strand, within Dshi_3982 at 41.656 kb on + strand, within Dshi_3982 at 41.707 kb on + strand, within Dshi_3982 at 41.972 kb on - strand at 42.080 kb on + strand, within Dshi_3983 at 42.095 kb on - strand, within Dshi_3983 at 42.133 kb on - strand, within Dshi_3983 at 42.178 kb on - strand, within Dshi_3983 at 42.178 kb on - strand, within Dshi_3983 at 42.364 kb on + strand, within Dshi_3983 at 42.392 kb on - strand, within Dshi_3983 at 42.492 kb on + strand, within Dshi_3983 at 42.607 kb on - strand, within Dshi_3983 at 42.665 kb on + strand, within Dshi_3983 at 42.747 kb on - strand, within Dshi_3983 at 42.760 kb on + strand, within Dshi_3983 at 42.791 kb on - strand, within Dshi_3983 at 42.807 kb on - strand, within Dshi_3983 at 42.993 kb on - strand at 42.993 kb on - strand at 43.008 kb on - strand at 43.009 kb on + strand at 43.009 kb on + strand at 43.009 kb on + strand at 43.009 kb on + strand at 43.009 kb on + strand at 43.081 kb on - strand at 43.351 kb on + strand, within Dshi_3984 at 43.376 kb on + strand, within Dshi_3984 at 43.485 kb on - strand at 43.510 kb on - strand at 43.556 kb on + strand at 43.569 kb on - strand at 43.588 kb on - strand at 43.591 kb on + strand at 43.599 kb on - strand at 43.605 kb on + strand at 43.620 kb on - strand at 43.628 kb on + strand at 43.646 kb on + strand, within Dshi_3985 at 43.765 kb on + strand, within Dshi_3985 at 43.765 kb on + strand, within Dshi_3985 at 43.785 kb on - strand, within Dshi_3985 at 43.790 kb on + strand, within Dshi_3985 at 43.800 kb on - strand, within Dshi_3985 at 43.814 kb on - strand, within Dshi_3985 at 43.815 kb on - strand, within Dshi_3985 at 43.853 kb on - strand, within Dshi_3985 at 43.853 kb on - strand, within Dshi_3985 at 43.853 kb on - strand, within Dshi_3985
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 41,656 + Dshi_3982 0.81 -2.9 41,656 + Dshi_3982 0.81 -0.7 41,707 + Dshi_3982 0.84 -0.7 41,972 - +0.2 42,080 + Dshi_3983 0.15 -0.6 42,095 - Dshi_3983 0.17 +1.9 42,133 - Dshi_3983 0.21 -0.1 42,178 - Dshi_3983 0.25 +0.4 42,178 - Dshi_3983 0.25 +0.1 42,364 + Dshi_3983 0.44 -1.3 42,392 - Dshi_3983 0.47 -1.1 42,492 + Dshi_3983 0.57 -1.2 42,607 - Dshi_3983 0.69 -0.7 42,665 + Dshi_3983 0.75 -0.4 42,747 - Dshi_3983 0.83 -0.9 42,760 + Dshi_3983 0.84 -0.1 42,791 - Dshi_3983 0.87 -0.6 42,807 - Dshi_3983 0.89 -0.4 42,993 - +0.8 42,993 - +0.8 43,008 - -0.8 43,009 + +1.1 43,009 + -0.4 43,009 + -0.4 43,009 + -0.3 43,009 + -0.0 43,081 - -0.2 43,351 + Dshi_3984 0.60 +0.8 43,376 + Dshi_3984 0.66 +1.2 43,485 - +0.5 43,510 - +0.1 43,556 + +1.2 43,569 - -0.4 43,588 - +0.3 43,591 + -1.1 43,599 - +0.1 43,605 + -0.1 43,620 - -0.2 43,628 + -0.5 43,646 + Dshi_3985 0.11 +0.1 43,765 + Dshi_3985 0.22 +0.6 43,765 + Dshi_3985 0.22 -0.3 43,785 - Dshi_3985 0.24 -0.6 43,790 + Dshi_3985 0.25 -0.0 43,800 - Dshi_3985 0.25 +0.7 43,814 - Dshi_3985 0.27 +1.5 43,815 - Dshi_3985 0.27 -0.4 43,853 - Dshi_3985 0.30 -1.8 43,853 - Dshi_3985 0.30 +2.5 43,853 - Dshi_3985 0.30 +0.6
Or see this region's nucleotide sequence