Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1885 and Dshi_1886 are separated by 7 nucleotides Dshi_1886 and Dshi_1887 are separated by 204 nucleotides Dshi_1887 and Dshi_1888 are separated by 97 nucleotides Dshi_1888 and Dshi_1889 overlap by 4 nucleotides
Dshi_1885: Dshi_1885 - monooxygenase FAD-binding (RefSeq), at 1,962,221 to 1,963,390
_1885
Dshi_1886: Dshi_1886 - exodeoxyribonuclease III Xth (RefSeq), at 1,963,398 to 1,964,177
_1886
Dshi_1887: Dshi_1887 - ribonuclease D (RefSeq), at 1,964,382 to 1,965,539
_1887
Dshi_1888: Dshi_1888 - phosphoribosylglycinamide formyltransferase (RefSeq), at 1,965,637 to 1,966,230
_1888
Dshi_1889: Dshi_1889 - phosphoribosylformylglycinamidine cyclo-ligase (RefSeq), at 1,966,227 to 1,967,288
_1889
Position (kb) 1964
1965
1966 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1963.387 kb on - strand at 1963.447 kb on - strand at 1963.482 kb on + strand, within Dshi_1886 at 1963.495 kb on - strand, within Dshi_1886 at 1963.911 kb on + strand, within Dshi_1886 at 1964.020 kb on + strand, within Dshi_1886 at 1964.028 kb on - strand, within Dshi_1886 at 1964.116 kb on - strand at 1964.118 kb on + strand at 1964.223 kb on + strand at 1964.312 kb on + strand at 1964.601 kb on - strand, within Dshi_1887 at 1964.676 kb on + strand, within Dshi_1887 at 1964.690 kb on - strand at 1964.973 kb on - strand, within Dshi_1887 at 1965.091 kb on + strand, within Dshi_1887 at 1965.287 kb on - strand, within Dshi_1887 at 1965.316 kb on - strand, within Dshi_1887 at 1965.532 kb on - strand at 1965.810 kb on + strand, within Dshi_1888 at 1965.821 kb on + strand, within Dshi_1888 at 1965.829 kb on - strand, within Dshi_1888 at 1965.859 kb on + strand, within Dshi_1888 at 1966.204 kb on + strand at 1966.391 kb on + strand, within Dshi_1889
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,963,387 - +0.0 1,963,447 - -0.1 1,963,482 + Dshi_1886 0.11 -0.6 1,963,495 - Dshi_1886 0.12 -0.5 1,963,911 + Dshi_1886 0.66 -0.5 1,964,020 + Dshi_1886 0.80 -0.5 1,964,028 - Dshi_1886 0.81 -0.4 1,964,116 - -0.5 1,964,118 + -0.5 1,964,223 + +0.4 1,964,312 + +0.4 1,964,601 - Dshi_1887 0.19 +0.2 1,964,676 + Dshi_1887 0.25 -0.0 1,964,690 - +0.8 1,964,973 - Dshi_1887 0.51 -0.0 1,965,091 + Dshi_1887 0.61 -0.0 1,965,287 - Dshi_1887 0.78 -0.2 1,965,316 - Dshi_1887 0.81 +0.3 1,965,532 - -1.5 1,965,810 + Dshi_1888 0.29 -5.2 1,965,821 + Dshi_1888 0.31 -3.5 1,965,829 - Dshi_1888 0.32 -3.6 1,965,859 + Dshi_1888 0.37 -3.0 1,966,204 + -2.5 1,966,391 + Dshi_1889 0.15 -3.3
Or see this region's nucleotide sequence