Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0322 and Dshi_0323 overlap by 4 nucleotides Dshi_0323 and Dshi_0324 are separated by 52 nucleotides Dshi_0324 and Dshi_0325 are separated by 87 nucleotides Dshi_0325 and Dshi_0326 are separated by 62 nucleotides
Dshi_0322: Dshi_0322 - putative phosphohistidine phosphatase, SixA (RefSeq), at 310,800 to 311,300
_0322
Dshi_0323: Dshi_0323 - hypothetical protein (RefSeq), at 311,297 to 311,920
_0323
Dshi_0324: Dshi_0324 - acetylglutamate kinase (RefSeq), at 311,973 to 312,836
_0324
Dshi_0325: Dshi_0325 - short-chain dehydrogenase/reductase SDR (RefSeq), at 312,924 to 313,655
_0325
Dshi_0326: Dshi_0326 - GTP-binding protein HSR1-related (RefSeq), at 313,718 to 314,371
_0326
Position (kb) 311
312
313 Strain fitness (log2 ratio) -1
0
1 at 311.516 kb on + strand, within Dshi_0323 at 311.523 kb on + strand, within Dshi_0323 at 311.977 kb on - strand at 312.893 kb on + strand at 312.893 kb on + strand at 312.901 kb on - strand at 312.901 kb on - strand at 312.901 kb on - strand at 312.901 kb on - strand at 312.937 kb on - strand at 313.027 kb on + strand, within Dshi_0325 at 313.027 kb on - strand, within Dshi_0325 at 313.187 kb on + strand, within Dshi_0325 at 313.343 kb on - strand, within Dshi_0325 at 313.356 kb on + strand, within Dshi_0325 at 313.370 kb on - strand, within Dshi_0325 at 313.383 kb on + strand, within Dshi_0325
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 311,516 + Dshi_0323 0.35 -1.3 311,523 + Dshi_0323 0.36 -1.0 311,977 - -0.1 312,893 + +1.2 312,893 + -0.2 312,901 - -0.7 312,901 - +0.2 312,901 - +0.8 312,901 - +0.5 312,937 - -1.2 313,027 + Dshi_0325 0.14 +0.3 313,027 - Dshi_0325 0.14 +0.3 313,187 + Dshi_0325 0.36 +0.1 313,343 - Dshi_0325 0.57 -0.0 313,356 + Dshi_0325 0.59 -0.0 313,370 - Dshi_0325 0.61 -0.3 313,383 + Dshi_0325 0.63 -0.9
Or see this region's nucleotide sequence