Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03210 and PGA1_c03220 overlap by 4 nucleotides PGA1_c03220 and PGA1_c03230 are separated by 59 nucleotides PGA1_c03230 and PGA1_c03240 are separated by 62 nucleotides PGA1_c03240 and PGA1_c03250 overlap by 4 nucleotides
PGA1_c03210: PGA1_c03210 - branched-chain amino acid transport protein azlD-like protein, at 308,374 to 308,709
_c03210
PGA1_c03220: PGA1_c03220 - branched-chain amino acid transport protein azlC-like protein, at 308,706 to 309,431
_c03220
PGA1_c03230: PGA1_c03230 - putative formate dehydrogenase, subunit FdhD, at 309,491 to 310,393
_c03230
PGA1_c03240: PGA1_c03240 - probable molybdopterin-guanine dinucleotide biosynthesis protein A, at 310,456 to 311,097
_c03240
PGA1_c03250: PGA1_c03250 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 311,094 to 311,585
_c03250
Position (kb) 309
310
311 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 308.524 kb on + strand, within PGA1_c03210 at 308.527 kb on + strand, within PGA1_c03210 at 308.567 kb on + strand, within PGA1_c03210 at 308.567 kb on - strand, within PGA1_c03210 at 308.575 kb on - strand, within PGA1_c03210 at 308.595 kb on + strand, within PGA1_c03210 at 308.648 kb on - strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.686 kb on + strand at 308.693 kb on - strand at 308.693 kb on - strand at 308.718 kb on - strand at 308.718 kb on - strand at 308.754 kb on - strand at 308.756 kb on + strand at 308.761 kb on - strand at 308.789 kb on - strand, within PGA1_c03220 at 308.803 kb on - strand at 308.816 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.868 kb on - strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.887 kb on - strand, within PGA1_c03220 at 308.957 kb on + strand, within PGA1_c03220 at 308.975 kb on - strand, within PGA1_c03220 at 308.981 kb on - strand, within PGA1_c03220 at 309.005 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.043 kb on - strand, within PGA1_c03220 at 309.068 kb on + strand, within PGA1_c03220 at 309.068 kb on + strand, within PGA1_c03220 at 309.082 kb on + strand, within PGA1_c03220 at 309.090 kb on - strand, within PGA1_c03220 at 309.154 kb on - strand, within PGA1_c03220 at 309.250 kb on + strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.268 kb on - strand, within PGA1_c03220 at 309.280 kb on - strand, within PGA1_c03220 at 309.304 kb on + strand, within PGA1_c03220 at 309.306 kb on - strand, within PGA1_c03220 at 309.459 kb on - strand at 309.460 kb on + strand at 309.460 kb on + strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.515 kb on - strand at 309.520 kb on - strand at 309.739 kb on + strand, within PGA1_c03230 at 309.749 kb on + strand, within PGA1_c03230 at 309.782 kb on + strand, within PGA1_c03230 at 309.827 kb on - strand, within PGA1_c03230 at 309.874 kb on - strand, within PGA1_c03230 at 309.927 kb on + strand, within PGA1_c03230 at 309.927 kb on + strand, within PGA1_c03230 at 309.929 kb on + strand, within PGA1_c03230 at 309.929 kb on + strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.986 kb on - strand, within PGA1_c03230 at 309.990 kb on + strand, within PGA1_c03230 at 309.990 kb on + strand, within PGA1_c03230 at 309.998 kb on - strand, within PGA1_c03230 at 309.998 kb on - strand, within PGA1_c03230 at 310.026 kb on + strand, within PGA1_c03230 at 310.045 kb on + strand, within PGA1_c03230 at 310.069 kb on + strand, within PGA1_c03230 at 310.153 kb on + strand, within PGA1_c03230 at 310.158 kb on + strand, within PGA1_c03230 at 310.166 kb on - strand, within PGA1_c03230 at 310.299 kb on - strand, within PGA1_c03230 at 310.348 kb on - strand at 310.440 kb on + strand at 310.639 kb on + strand, within PGA1_c03240 at 310.675 kb on - strand, within PGA1_c03240 at 310.707 kb on - strand, within PGA1_c03240 at 310.721 kb on - strand, within PGA1_c03240 at 310.811 kb on + strand, within PGA1_c03240 at 310.816 kb on + strand, within PGA1_c03240 at 310.922 kb on + strand, within PGA1_c03240 at 310.971 kb on - strand, within PGA1_c03240 at 310.981 kb on - strand, within PGA1_c03240 at 310.990 kb on + strand, within PGA1_c03240 at 310.998 kb on - strand, within PGA1_c03240 at 311.024 kb on - strand, within PGA1_c03240 at 311.024 kb on - strand, within PGA1_c03240 at 311.049 kb on + strand at 311.057 kb on - strand at 311.076 kb on + strand at 311.099 kb on + strand at 311.099 kb on + strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.109 kb on + strand at 311.124 kb on - strand at 311.179 kb on + strand, within PGA1_c03250 at 311.208 kb on + strand, within PGA1_c03250 at 311.221 kb on - strand, within PGA1_c03250 at 311.270 kb on + strand, within PGA1_c03250 at 311.331 kb on + strand, within PGA1_c03250 at 311.376 kb on + strand, within PGA1_c03250
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 308,524 + PGA1_c03210 0.45 +1.2 308,527 + PGA1_c03210 0.46 +0.4 308,567 + PGA1_c03210 0.57 +0.3 308,567 - PGA1_c03210 0.57 -1.0 308,575 - PGA1_c03210 0.60 +0.2 308,595 + PGA1_c03210 0.66 +0.6 308,648 - PGA1_c03210 0.82 +0.6 308,675 + PGA1_c03210 0.90 -0.6 308,675 + PGA1_c03210 0.90 +0.2 308,675 + PGA1_c03210 0.90 -1.7 308,675 + PGA1_c03210 0.90 -0.2 308,675 + PGA1_c03210 0.90 -0.5 308,675 + PGA1_c03210 0.90 -0.0 308,683 - -0.4 308,683 - -0.1 308,683 - +1.2 308,683 - +0.3 308,683 - -3.2 308,686 + -0.8 308,693 - -0.6 308,693 - -1.5 308,718 - -3.2 308,718 - -0.8 308,754 - +0.6 308,756 + -0.9 308,761 - -0.2 308,789 - PGA1_c03220 0.11 -0.3 308,803 - -1.4 308,816 - PGA1_c03220 0.15 -1.7 308,826 - PGA1_c03220 0.17 +1.0 308,826 - PGA1_c03220 0.17 +0.0 308,826 - PGA1_c03220 0.17 -0.7 308,826 - PGA1_c03220 0.17 -0.3 308,826 - PGA1_c03220 0.17 -2.0 308,868 - PGA1_c03220 0.22 -2.2 308,879 + PGA1_c03220 0.24 -1.4 308,879 + PGA1_c03220 0.24 +1.0 308,879 + PGA1_c03220 0.24 -0.4 308,887 - PGA1_c03220 0.25 -1.7 308,957 + PGA1_c03220 0.35 -1.3 308,975 - PGA1_c03220 0.37 -1.3 308,981 - PGA1_c03220 0.38 -0.1 309,005 + PGA1_c03220 0.41 -0.1 309,035 + PGA1_c03220 0.45 -1.2 309,035 + PGA1_c03220 0.45 -0.0 309,035 + PGA1_c03220 0.45 -2.8 309,043 - PGA1_c03220 0.46 -1.2 309,068 + PGA1_c03220 0.50 -2.8 309,068 + PGA1_c03220 0.50 +0.3 309,082 + PGA1_c03220 0.52 +1.2 309,090 - PGA1_c03220 0.53 -0.8 309,154 - PGA1_c03220 0.62 -0.1 309,250 + PGA1_c03220 0.75 -0.2 309,258 - PGA1_c03220 0.76 -0.3 309,258 - PGA1_c03220 0.76 -0.3 309,258 - PGA1_c03220 0.76 +0.6 309,268 - PGA1_c03220 0.77 +0.4 309,280 - PGA1_c03220 0.79 -0.3 309,304 + PGA1_c03220 0.82 +0.2 309,306 - PGA1_c03220 0.83 +0.8 309,459 - +0.3 309,460 + +0.1 309,460 + -2.2 309,468 - -1.0 309,468 - +0.6 309,468 - -1.0 309,468 - +0.7 309,515 - +0.9 309,520 - +2.9 309,739 + PGA1_c03230 0.27 +0.7 309,749 + PGA1_c03230 0.29 +2.2 309,782 + PGA1_c03230 0.32 +0.8 309,827 - PGA1_c03230 0.37 +1.2 309,874 - PGA1_c03230 0.42 -0.5 309,927 + PGA1_c03230 0.48 -0.1 309,927 + PGA1_c03230 0.48 +1.7 309,929 + PGA1_c03230 0.49 -0.2 309,929 + PGA1_c03230 0.49 +1.0 309,937 - PGA1_c03230 0.49 +1.1 309,937 - PGA1_c03230 0.49 +1.9 309,937 - PGA1_c03230 0.49 +0.8 309,986 - PGA1_c03230 0.55 +0.0 309,990 + PGA1_c03230 0.55 -0.2 309,990 + PGA1_c03230 0.55 +0.2 309,998 - PGA1_c03230 0.56 -0.7 309,998 - PGA1_c03230 0.56 +1.0 310,026 + PGA1_c03230 0.59 +1.6 310,045 + PGA1_c03230 0.61 +1.2 310,069 + PGA1_c03230 0.64 +1.3 310,153 + PGA1_c03230 0.73 +2.2 310,158 + PGA1_c03230 0.74 +2.2 310,166 - PGA1_c03230 0.75 -2.2 310,299 - PGA1_c03230 0.89 +1.0 310,348 - -1.8 310,440 + -1.8 310,639 + PGA1_c03240 0.29 +1.3 310,675 - PGA1_c03240 0.34 -1.3 310,707 - PGA1_c03240 0.39 -0.2 310,721 - PGA1_c03240 0.41 +2.6 310,811 + PGA1_c03240 0.55 +0.6 310,816 + PGA1_c03240 0.56 +0.2 310,922 + PGA1_c03240 0.73 +1.0 310,971 - PGA1_c03240 0.80 -0.7 310,981 - PGA1_c03240 0.82 +1.5 310,990 + PGA1_c03240 0.83 -0.4 310,998 - PGA1_c03240 0.84 +1.1 311,024 - PGA1_c03240 0.88 -0.7 311,024 - PGA1_c03240 0.88 -0.2 311,049 + +0.1 311,057 - -1.1 311,076 + -0.6 311,099 + +0.2 311,099 + -1.4 311,107 - +0.2 311,107 - -1.5 311,107 - +0.3 311,107 - +0.7 311,107 - -1.7 311,109 + -0.2 311,124 - +1.2 311,179 + PGA1_c03250 0.17 -0.4 311,208 + PGA1_c03250 0.23 -0.2 311,221 - PGA1_c03250 0.26 -0.4 311,270 + PGA1_c03250 0.36 -0.3 311,331 + PGA1_c03250 0.48 +0.6 311,376 + PGA1_c03250 0.57 +1.2
Or see this region's nucleotide sequence