Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24270 and PGA1_c24280 are separated by 393 nucleotides PGA1_c24280 and PGA1_c24290 are separated by 107 nucleotides PGA1_c24290 and PGA1_c24300 overlap by 4 nucleotides
PGA1_c24270: PGA1_c24270 - Uncharacterized conserved protein, at 2,532,581 to 2,533,099
_c24270
PGA1_c24280: PGA1_c24280 - uncharacterized GST-like protein YghU, at 2,533,493 to 2,534,374
_c24280
PGA1_c24290: PGA1_c24290 - hypothetical protein, at 2,534,482 to 2,535,279
_c24290
PGA1_c24300: PGA1_c24300 - Uracil-DNA glycosylase, at 2,535,276 to 2,535,869
_c24300
Position (kb)
2533
2534
2535 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2532.494 kb on + strand at 2532.494 kb on + strand at 2532.494 kb on + strand at 2532.497 kb on - strand at 2532.550 kb on + strand at 2532.562 kb on + strand at 2532.579 kb on + strand at 2532.579 kb on + strand at 2532.587 kb on - strand at 2532.587 kb on - strand at 2532.589 kb on + strand at 2532.657 kb on + strand, within PGA1_c24270 at 2532.657 kb on + strand, within PGA1_c24270 at 2532.665 kb on - strand, within PGA1_c24270 at 2532.702 kb on - strand, within PGA1_c24270 at 2532.705 kb on - strand, within PGA1_c24270 at 2532.740 kb on - strand, within PGA1_c24270 at 2532.763 kb on + strand, within PGA1_c24270 at 2532.771 kb on - strand, within PGA1_c24270 at 2532.771 kb on - strand, within PGA1_c24270 at 2532.771 kb on - strand, within PGA1_c24270 at 2532.771 kb on - strand, within PGA1_c24270 at 2532.771 kb on - strand, within PGA1_c24270 at 2532.771 kb on - strand, within PGA1_c24270 at 2532.773 kb on + strand, within PGA1_c24270 at 2532.781 kb on - strand, within PGA1_c24270 at 2532.781 kb on - strand, within PGA1_c24270 at 2532.781 kb on - strand, within PGA1_c24270 at 2532.953 kb on + strand, within PGA1_c24270 at 2532.998 kb on + strand, within PGA1_c24270 at 2532.999 kb on - strand, within PGA1_c24270 at 2533.006 kb on + strand, within PGA1_c24270 at 2533.012 kb on + strand, within PGA1_c24270 at 2533.054 kb on + strand at 2533.130 kb on - strand at 2533.240 kb on + strand at 2533.245 kb on + strand at 2533.270 kb on + strand at 2533.309 kb on + strand at 2533.318 kb on + strand at 2533.366 kb on + strand at 2533.366 kb on - strand at 2533.513 kb on - strand at 2533.540 kb on + strand at 2533.548 kb on - strand at 2533.761 kb on - strand, within PGA1_c24280 at 2533.782 kb on - strand, within PGA1_c24280 at 2533.905 kb on - strand, within PGA1_c24280 at 2533.927 kb on - strand, within PGA1_c24280 at 2533.950 kb on - strand, within PGA1_c24280 at 2533.953 kb on + strand, within PGA1_c24280 at 2534.057 kb on - strand, within PGA1_c24280 at 2534.136 kb on + strand, within PGA1_c24280 at 2534.202 kb on + strand, within PGA1_c24280 at 2534.204 kb on - strand, within PGA1_c24280 at 2534.212 kb on + strand, within PGA1_c24280 at 2534.215 kb on - strand, within PGA1_c24280 at 2534.217 kb on + strand, within PGA1_c24280 at 2534.225 kb on - strand, within PGA1_c24280 at 2534.225 kb on - strand, within PGA1_c24280 at 2534.263 kb on + strand, within PGA1_c24280 at 2534.349 kb on - strand at 2534.349 kb on - strand at 2534.421 kb on + strand at 2534.474 kb on - strand at 2534.672 kb on + strand, within PGA1_c24290 at 2534.672 kb on + strand, within PGA1_c24290 at 2534.672 kb on + strand, within PGA1_c24290 at 2534.672 kb on + strand, within PGA1_c24290 at 2534.680 kb on - strand, within PGA1_c24290 at 2534.680 kb on - strand, within PGA1_c24290 at 2534.767 kb on + strand, within PGA1_c24290 at 2534.783 kb on - strand, within PGA1_c24290 at 2534.995 kb on - strand, within PGA1_c24290 at 2535.012 kb on - strand, within PGA1_c24290 at 2535.022 kb on + strand, within PGA1_c24290 at 2535.121 kb on - strand, within PGA1_c24290 at 2535.228 kb on - strand at 2535.285 kb on - strand at 2535.298 kb on - strand at 2535.343 kb on + strand, within PGA1_c24300
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 2,532,494 + -0.9 2,532,494 + -1.9 2,532,494 + -1.2 2,532,497 - -2.4 2,532,550 + +0.9 2,532,562 + -0.1 2,532,579 + -1.4 2,532,579 + -1.7 2,532,587 - -0.7 2,532,587 - -1.7 2,532,589 + -0.4 2,532,657 + PGA1_c24270 0.15 -2.4 2,532,657 + PGA1_c24270 0.15 +0.2 2,532,665 - PGA1_c24270 0.16 -1.1 2,532,702 - PGA1_c24270 0.23 -2.7 2,532,705 - PGA1_c24270 0.24 -1.1 2,532,740 - PGA1_c24270 0.31 -0.4 2,532,763 + PGA1_c24270 0.35 -2.5 2,532,771 - PGA1_c24270 0.37 -1.4 2,532,771 - PGA1_c24270 0.37 -0.1 2,532,771 - PGA1_c24270 0.37 -1.1 2,532,771 - PGA1_c24270 0.37 -2.1 2,532,771 - PGA1_c24270 0.37 -3.6 2,532,771 - PGA1_c24270 0.37 -0.5 2,532,773 + PGA1_c24270 0.37 -2.4 2,532,781 - PGA1_c24270 0.39 -0.9 2,532,781 - PGA1_c24270 0.39 -1.1 2,532,781 - PGA1_c24270 0.39 -1.4 2,532,953 + PGA1_c24270 0.72 -2.2 2,532,998 + PGA1_c24270 0.80 -0.4 2,532,999 - PGA1_c24270 0.81 -0.2 2,533,006 + PGA1_c24270 0.82 -2.4 2,533,012 + PGA1_c24270 0.83 -1.7 2,533,054 + +0.2 2,533,130 - -2.2 2,533,240 + -2.0 2,533,245 + -3.3 2,533,270 + -1.7 2,533,309 + -1.0 2,533,318 + -1.1 2,533,366 + -1.2 2,533,366 - -0.1 2,533,513 - -0.1 2,533,540 + -3.9 2,533,548 - -2.5 2,533,761 - PGA1_c24280 0.30 -0.4 2,533,782 - PGA1_c24280 0.33 +0.9 2,533,905 - PGA1_c24280 0.47 -1.6 2,533,927 - PGA1_c24280 0.49 -2.2 2,533,950 - PGA1_c24280 0.52 +0.7 2,533,953 + PGA1_c24280 0.52 -1.5 2,534,057 - PGA1_c24280 0.64 -1.7 2,534,136 + PGA1_c24280 0.73 -1.1 2,534,202 + PGA1_c24280 0.80 -2.6 2,534,204 - PGA1_c24280 0.81 +0.1 2,534,212 + PGA1_c24280 0.82 -0.2 2,534,215 - PGA1_c24280 0.82 -1.7 2,534,217 + PGA1_c24280 0.82 -3.0 2,534,225 - PGA1_c24280 0.83 +0.3 2,534,225 - PGA1_c24280 0.83 -0.3 2,534,263 + PGA1_c24280 0.87 -1.7 2,534,349 - -2.2 2,534,349 - -0.7 2,534,421 + -1.1 2,534,474 - -1.2 2,534,672 + PGA1_c24290 0.24 -1.4 2,534,672 + PGA1_c24290 0.24 +0.6 2,534,672 + PGA1_c24290 0.24 +0.1 2,534,672 + PGA1_c24290 0.24 -0.5 2,534,680 - PGA1_c24290 0.25 -2.4 2,534,680 - PGA1_c24290 0.25 -1.4 2,534,767 + PGA1_c24290 0.36 -1.9 2,534,783 - PGA1_c24290 0.38 -1.4 2,534,995 - PGA1_c24290 0.64 +0.6 2,535,012 - PGA1_c24290 0.66 -2.3 2,535,022 + PGA1_c24290 0.68 -1.2 2,535,121 - PGA1_c24290 0.80 -2.1 2,535,228 - -0.9 2,535,285 - -0.4 2,535,298 - -0.8 2,535,343 + PGA1_c24300 0.11 -0.3
Or see this region's nucleotide sequence