Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3057 and Dsui_3058 are separated by 24 nucleotides Dsui_3058 and Dsui_3059 are separated by 79 nucleotides Dsui_3059 and Dsui_3060 are separated by 91 nucleotides Dsui_3060 and Dsui_3061 are separated by 35 nucleotides
Dsui_3057: Dsui_3057 - phosphoglucosamine mutase, at 3,271,779 to 3,273,134
_3057
Dsui_3058: Dsui_3058 - dihydropteroate synthase, at 3,273,159 to 3,273,983
_3058
Dsui_3059: Dsui_3059 - ATP-dependent metalloprotease FtsH, at 3,274,063 to 3,275,946
_3059
Dsui_3060: Dsui_3060 - 23S rRNA methylase, at 3,276,038 to 3,276,670
_3060
Dsui_3061: Dsui_3061 - putative RNA-binding protein, YhbY family, at 3,276,706 to 3,277,062
_3061
Position (kb)
3274
3275
3276 Strain fitness (log2 ratio)
-2
-1
0
1 at 3273.174 kb on - strand at 3273.174 kb on - strand at 3273.174 kb on - strand at 3273.989 kb on - strand at 3274.022 kb on - strand at 3274.022 kb on - strand at 3275.971 kb on - strand at 3276.035 kb on + strand at 3276.040 kb on + strand at 3276.055 kb on - strand at 3276.114 kb on - strand, within Dsui_3060 at 3276.138 kb on - strand, within Dsui_3060 at 3276.138 kb on - strand, within Dsui_3060 at 3276.138 kb on - strand, within Dsui_3060 at 3276.171 kb on + strand, within Dsui_3060 at 3276.253 kb on + strand, within Dsui_3060 at 3276.253 kb on + strand at 3276.253 kb on + strand, within Dsui_3060 at 3276.261 kb on - strand, within Dsui_3060 at 3276.261 kb on - strand, within Dsui_3060 at 3276.261 kb on - strand, within Dsui_3060 at 3276.261 kb on - strand, within Dsui_3060 at 3276.265 kb on - strand, within Dsui_3060 at 3276.416 kb on - strand, within Dsui_3060 at 3276.467 kb on - strand, within Dsui_3060 at 3276.528 kb on + strand at 3276.563 kb on - strand, within Dsui_3060 at 3276.563 kb on - strand, within Dsui_3060 at 3276.565 kb on + strand at 3276.573 kb on - strand, within Dsui_3060 at 3276.573 kb on - strand, within Dsui_3060 at 3276.639 kb on - strand at 3276.795 kb on + strand, within Dsui_3061 at 3276.803 kb on - strand, within Dsui_3061 at 3276.852 kb on + strand, within Dsui_3061 at 3276.860 kb on - strand, within Dsui_3061
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 3,273,174 - +0.1 3,273,174 - +0.3 3,273,174 - +0.2 3,273,989 - +0.7 3,274,022 - +0.1 3,274,022 - -0.3 3,275,971 - -0.2 3,276,035 + +0.7 3,276,040 + -0.8 3,276,055 - +0.2 3,276,114 - Dsui_3060 0.12 +0.4 3,276,138 - Dsui_3060 0.16 -0.3 3,276,138 - Dsui_3060 0.16 +0.4 3,276,138 - Dsui_3060 0.16 +0.6 3,276,171 + Dsui_3060 0.21 -0.4 3,276,253 + Dsui_3060 0.34 -0.4 3,276,253 + -0.1 3,276,253 + Dsui_3060 0.34 +1.4 3,276,261 - Dsui_3060 0.35 -0.1 3,276,261 - Dsui_3060 0.35 -0.0 3,276,261 - Dsui_3060 0.35 +0.1 3,276,261 - Dsui_3060 0.35 +0.2 3,276,265 - Dsui_3060 0.36 +0.4 3,276,416 - Dsui_3060 0.60 -0.2 3,276,467 - Dsui_3060 0.68 -0.3 3,276,528 + -2.3 3,276,563 - Dsui_3060 0.83 -1.3 3,276,563 - Dsui_3060 0.83 -0.0 3,276,565 + +1.3 3,276,573 - Dsui_3060 0.85 +0.2 3,276,573 - Dsui_3060 0.85 +0.2 3,276,639 - -0.2 3,276,795 + Dsui_3061 0.25 +0.1 3,276,803 - Dsui_3061 0.27 +0.2 3,276,852 + Dsui_3061 0.41 +0.3 3,276,860 - Dsui_3061 0.43 +1.0
Or see this region's nucleotide sequence