Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2868 and Dsui_2869 overlap by 17 nucleotides Dsui_2869 and Dsui_2870 are separated by 99 nucleotides
Dsui_2868: Dsui_2868 - tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain protein, at 3,061,891 to 3,063,858
_2868
Dsui_2869: Dsui_2869 - transcriptional regulator, at 3,063,842 to 3,064,813
_2869
Dsui_2870: Dsui_2870 - putative permease, at 3,064,913 to 3,065,839
_2870
Position (kb)
3063
3064
3065 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3062.893 kb on + strand at 3062.956 kb on + strand, within Dsui_2868 at 3063.078 kb on - strand, within Dsui_2868 at 3063.130 kb on - strand at 3063.243 kb on + strand, within Dsui_2868 at 3063.313 kb on + strand at 3063.545 kb on + strand, within Dsui_2868 at 3063.569 kb on + strand, within Dsui_2868 at 3063.668 kb on + strand at 3063.668 kb on + strand at 3063.736 kb on + strand at 3063.794 kb on + strand at 3063.794 kb on + strand at 3063.825 kb on + strand at 3063.833 kb on - strand at 3063.946 kb on - strand, within Dsui_2869 at 3063.947 kb on - strand, within Dsui_2869 at 3064.002 kb on + strand, within Dsui_2869 at 3064.194 kb on - strand, within Dsui_2869 at 3064.356 kb on + strand, within Dsui_2869 at 3064.465 kb on + strand, within Dsui_2869 at 3064.545 kb on - strand, within Dsui_2869 at 3064.632 kb on - strand, within Dsui_2869 at 3064.633 kb on + strand at 3064.881 kb on - strand at 3065.006 kb on + strand, within Dsui_2870 at 3065.133 kb on + strand, within Dsui_2870 at 3065.154 kb on + strand, within Dsui_2870 at 3065.267 kb on + strand, within Dsui_2870 at 3065.326 kb on + strand, within Dsui_2870 at 3065.467 kb on + strand, within Dsui_2870 at 3065.482 kb on + strand, within Dsui_2870 at 3065.594 kb on + strand, within Dsui_2870 at 3065.757 kb on + strand at 3065.805 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 3,062,893 + +0.6 3,062,956 + Dsui_2868 0.54 -0.2 3,063,078 - Dsui_2868 0.60 +0.2 3,063,130 - -1.7 3,063,243 + Dsui_2868 0.69 -0.7 3,063,313 + +0.7 3,063,545 + Dsui_2868 0.84 -0.1 3,063,569 + Dsui_2868 0.85 +0.3 3,063,668 + -0.4 3,063,668 + +0.4 3,063,736 + -1.3 3,063,794 + -1.5 3,063,794 + -1.0 3,063,825 + -0.2 3,063,833 - -1.4 3,063,946 - Dsui_2869 0.11 -0.7 3,063,947 - Dsui_2869 0.11 -3.0 3,064,002 + Dsui_2869 0.16 -0.0 3,064,194 - Dsui_2869 0.36 -0.5 3,064,356 + Dsui_2869 0.53 -1.3 3,064,465 + Dsui_2869 0.64 +0.3 3,064,545 - Dsui_2869 0.72 -1.1 3,064,632 - Dsui_2869 0.81 -0.2 3,064,633 + -1.8 3,064,881 - +0.6 3,065,006 + Dsui_2870 0.10 +0.4 3,065,133 + Dsui_2870 0.24 -0.7 3,065,154 + Dsui_2870 0.26 -0.6 3,065,267 + Dsui_2870 0.38 -0.3 3,065,326 + Dsui_2870 0.45 -0.2 3,065,467 + Dsui_2870 0.60 -0.5 3,065,482 + Dsui_2870 0.61 +0.1 3,065,594 + Dsui_2870 0.73 +0.3 3,065,757 + -0.0 3,065,805 + +0.7
Or see this region's nucleotide sequence