Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2866 and Dsui_2867 are separated by 271 nucleotides Dsui_2867 and Dsui_2868 are separated by 46 nucleotides
Dsui_2866: Dsui_2866 - outer membrane receptor for ferrienterochelin and colicins, at 3,058,922 to 3,060,874
_2866
Dsui_2867: Dsui_2867 - surface antigen family protein, at 3,061,146 to 3,061,844
_2867
Dsui_2868: Dsui_2868 - tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain protein, at 3,061,891 to 3,063,858
_2868
Position (kb)
3061
3062 Strain fitness (log2 ratio)
-1
0
1
2 at 3060.302 kb on - strand, within Dsui_2866 at 3060.468 kb on - strand at 3060.482 kb on - strand, within Dsui_2866 at 3060.544 kb on - strand, within Dsui_2866 at 3060.605 kb on - strand, within Dsui_2866 at 3060.605 kb on - strand, within Dsui_2866 at 3060.716 kb on - strand at 3060.797 kb on + strand at 3060.891 kb on - strand at 3061.082 kb on + strand at 3061.136 kb on + strand at 3061.424 kb on + strand, within Dsui_2867 at 3061.424 kb on + strand, within Dsui_2867 at 3061.472 kb on + strand, within Dsui_2867 at 3061.555 kb on - strand at 3061.750 kb on + strand, within Dsui_2867 at 3061.755 kb on + strand at 3061.805 kb on + strand at 3061.888 kb on + strand at 3061.938 kb on + strand at 3062.018 kb on + strand at 3062.256 kb on + strand, within Dsui_2868 at 3062.366 kb on + strand, within Dsui_2868 at 3062.389 kb on + strand, within Dsui_2868 at 3062.730 kb on - strand, within Dsui_2868 at 3062.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 3,060,302 - Dsui_2866 0.71 -0.3 3,060,468 - +1.3 3,060,482 - Dsui_2866 0.80 -0.2 3,060,544 - Dsui_2866 0.83 -0.3 3,060,605 - Dsui_2866 0.86 +0.4 3,060,605 - Dsui_2866 0.86 -0.5 3,060,716 - +0.3 3,060,797 + +2.2 3,060,891 - -0.8 3,061,082 + +0.5 3,061,136 + +0.1 3,061,424 + Dsui_2867 0.40 -0.2 3,061,424 + Dsui_2867 0.40 -0.4 3,061,472 + Dsui_2867 0.47 -1.5 3,061,555 - -1.5 3,061,750 + Dsui_2867 0.86 -0.4 3,061,755 + -1.4 3,061,805 + +0.6 3,061,888 + +0.2 3,061,938 + -0.4 3,062,018 + -0.6 3,062,256 + Dsui_2868 0.19 +0.3 3,062,366 + Dsui_2868 0.24 +0.5 3,062,389 + Dsui_2868 0.25 +0.4 3,062,730 - Dsui_2868 0.43 -0.6 3,062,839 - -0.2
Or see this region's nucleotide sequence