Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2592 and Dsui_2593 are separated by 27 nucleotides Dsui_2593 and Dsui_2594 are separated by 12 nucleotides Dsui_2594 and Dsui_2595 are separated by 16 nucleotides
Dsui_2592: Dsui_2592 - type II secretory pathway, component PulF, at 2,747,608 to 2,748,720
_2592
Dsui_2593: Dsui_2593 - PilS N terminal protein, at 2,748,748 to 2,749,287
_2593
Dsui_2594: Dsui_2594 - type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 2,749,300 to 2,750,256
_2594
Dsui_2595: Dsui_2595 - type II secretory pathway, pseudopilin PulG, at 2,750,273 to 2,751,646
_2595
Position (kb)
2748
2749
2750 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2747.771 kb on - strand, within Dsui_2592 at 2747.801 kb on - strand, within Dsui_2592 at 2747.989 kb on + strand, within Dsui_2592 at 2748.044 kb on - strand, within Dsui_2592 at 2748.044 kb on - strand, within Dsui_2592 at 2748.046 kb on + strand, within Dsui_2592 at 2748.065 kb on - strand, within Dsui_2592 at 2748.084 kb on + strand, within Dsui_2592 at 2748.261 kb on - strand, within Dsui_2592 at 2748.314 kb on - strand, within Dsui_2592 at 2748.314 kb on - strand, within Dsui_2592 at 2748.324 kb on - strand, within Dsui_2592 at 2748.334 kb on + strand, within Dsui_2592 at 2748.357 kb on + strand, within Dsui_2592 at 2748.373 kb on - strand, within Dsui_2592 at 2748.487 kb on - strand, within Dsui_2592 at 2748.638 kb on - strand at 2748.658 kb on - strand at 2748.669 kb on - strand at 2748.678 kb on + strand at 2748.683 kb on + strand at 2748.720 kb on + strand at 2748.818 kb on - strand, within Dsui_2593 at 2748.820 kb on - strand, within Dsui_2593 at 2748.834 kb on + strand, within Dsui_2593 at 2748.842 kb on - strand, within Dsui_2593 at 2748.882 kb on + strand, within Dsui_2593 at 2748.890 kb on - strand, within Dsui_2593 at 2748.890 kb on - strand, within Dsui_2593 at 2748.890 kb on - strand, within Dsui_2593 at 2748.895 kb on - strand, within Dsui_2593 at 2749.105 kb on + strand, within Dsui_2593 at 2749.126 kb on + strand, within Dsui_2593 at 2749.199 kb on - strand at 2749.210 kb on + strand, within Dsui_2593 at 2749.210 kb on + strand, within Dsui_2593 at 2749.218 kb on - strand, within Dsui_2593 at 2749.235 kb on - strand at 2749.309 kb on + strand at 2749.392 kb on + strand at 2749.400 kb on - strand, within Dsui_2594 at 2749.580 kb on + strand, within Dsui_2594 at 2749.704 kb on + strand, within Dsui_2594 at 2749.732 kb on - strand, within Dsui_2594 at 2749.742 kb on + strand, within Dsui_2594 at 2749.742 kb on + strand, within Dsui_2594 at 2749.742 kb on + strand, within Dsui_2594 at 2749.747 kb on + strand, within Dsui_2594 at 2749.747 kb on + strand, within Dsui_2594 at 2749.748 kb on + strand, within Dsui_2594 at 2749.750 kb on - strand, within Dsui_2594 at 2749.755 kb on - strand, within Dsui_2594 at 2749.800 kb on - strand, within Dsui_2594 at 2749.938 kb on + strand, within Dsui_2594
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 2,747,771 - Dsui_2592 0.15 +0.1 2,747,801 - Dsui_2592 0.17 +0.6 2,747,989 + Dsui_2592 0.34 -0.8 2,748,044 - Dsui_2592 0.39 +0.5 2,748,044 - Dsui_2592 0.39 -0.5 2,748,046 + Dsui_2592 0.39 +0.6 2,748,065 - Dsui_2592 0.41 -0.2 2,748,084 + Dsui_2592 0.43 -0.8 2,748,261 - Dsui_2592 0.59 +1.0 2,748,314 - Dsui_2592 0.63 -0.6 2,748,314 - Dsui_2592 0.63 +0.5 2,748,324 - Dsui_2592 0.64 +0.6 2,748,334 + Dsui_2592 0.65 -1.1 2,748,357 + Dsui_2592 0.67 +1.8 2,748,373 - Dsui_2592 0.69 -0.3 2,748,487 - Dsui_2592 0.79 +0.5 2,748,638 - -2.1 2,748,658 - -0.2 2,748,669 - +0.7 2,748,678 + -0.5 2,748,683 + -0.3 2,748,720 + +0.0 2,748,818 - Dsui_2593 0.13 -0.0 2,748,820 - Dsui_2593 0.13 -1.1 2,748,834 + Dsui_2593 0.16 -0.9 2,748,842 - Dsui_2593 0.17 -0.6 2,748,882 + Dsui_2593 0.25 -1.1 2,748,890 - Dsui_2593 0.26 +0.0 2,748,890 - Dsui_2593 0.26 -0.7 2,748,890 - Dsui_2593 0.26 -2.1 2,748,895 - Dsui_2593 0.27 -0.8 2,749,105 + Dsui_2593 0.66 -1.7 2,749,126 + Dsui_2593 0.70 +1.0 2,749,199 - +1.5 2,749,210 + Dsui_2593 0.86 -1.9 2,749,210 + Dsui_2593 0.86 +0.2 2,749,218 - Dsui_2593 0.87 -0.8 2,749,235 - +1.3 2,749,309 + -1.0 2,749,392 + -0.2 2,749,400 - Dsui_2594 0.10 +1.3 2,749,580 + Dsui_2594 0.29 +0.8 2,749,704 + Dsui_2594 0.42 -0.1 2,749,732 - Dsui_2594 0.45 -0.5 2,749,742 + Dsui_2594 0.46 +0.5 2,749,742 + Dsui_2594 0.46 -0.5 2,749,742 + Dsui_2594 0.46 +1.4 2,749,747 + Dsui_2594 0.47 +1.0 2,749,747 + Dsui_2594 0.47 +0.0 2,749,748 + Dsui_2594 0.47 +0.0 2,749,750 - Dsui_2594 0.47 -0.5 2,749,755 - Dsui_2594 0.48 +1.0 2,749,800 - Dsui_2594 0.52 -0.8 2,749,938 + Dsui_2594 0.67 -2.3
Or see this region's nucleotide sequence